新阿洛酮糖-6-磷酸去磷酸化酶，包含其制备阿洛酮糖的组合物，和用其制备阿洛酮糖的方法与流程

本发明涉及一种新型的酶，其将阿洛酮糖-6-磷酸去磷酸化，以制备阿洛酮糖，一种组合物，其包含所述酶，用于制备阿洛酮糖，一种使用所述酶制备阿洛酮糖的方法。
背景技术：
已知D-阿洛酮糖-3-差向异构酶(D-Psicose-3-epimerase,EC5.1.3.30)和D-塔格糖-3-差向异构酶(D-tagatose-3-epimerase,EC5.1.3.31)是催化D-果糖的3-差向异构化以制备D-阿洛酮糖的酶。当使用该酶并通过单一酶促反应制备D-阿洛酮糖时，底物(即D-果糖)与产物(即D-阿洛酮糖)之间的反应平衡(reactionequilibrium)以恒定水平(产物/底物＝约20％-35％)存在。因此，制备高纯度的D-阿洛酮糖需要额外的处理，以从酶反应产物中分离和去除相对高浓度的D-果糖。另外，与淀粉或者葡萄糖相比，果糖是一种相对昂贵的原料，当果糖用作原料时，阿洛酮糖和塔格糖的成本率增加。因此，已报道了许多关于使用淀粉或葡萄糖(即相对经济的原料)进行反应，制备阿洛酮糖和塔格糖的研究(KR10-2018-0004023,WO2018-112139,WO2017-059278,WO2018-129275)。同时，Chan等人(2008.Biochemistry.47:9608-9617)报道了衍生自酿脓链球菌(Streptococcuspyogenes)的D-核酮糖-5-磷酸-3-差向异构酶(D-ribulose-5-phosphate-3-epimerase,EC5.1.3.1)和衍生自大肠杆菌(E.coli)的D-阿洛酮糖-6-磷酸-3-差向异构酶(D-psicose-6-phosphate-3-epimerase,EC5.1.3.-)，其能够催化D-果糖-6-磷酸(D-fructose-6-phosphate)和D-阿洛酮糖-6-磷酸(D-psicose-6-phosphate)的3-差向异构化。然而，由于耐热性较差，这些酶在工业上不适用。技术实现要素：在这样的情况下，本申请的发明人认真地进行了研究，以开发一种以经济的方式在工业规模上提高阿洛酮糖的转化率的方法。结果，本申请的发明人发现，将廉价的原料蔗糖或淀粉(例如麦芽糖糊精)转化为阿洛酮糖-6-磷酸之后，本发明使用阿洛酮糖-6-磷酸磷酸酶(psicose-6-phosphatephosphatase)，其对阿洛酮糖-6-磷酸具有特异性，并参与不可逆的反应途径，可以通过一锅法酶转化(one-potenzymaticconversions)产生阿洛酮糖，并显著提高阿洛酮糖的转化率，所述酶转化使用多种参与阿洛酮糖生产途径的酶。因此，基于这一发现，完成了本发明。与常用的已知的阿洛酮糖-6-磷酸磷酸酶相比(Panoramicviewofasuperfamilyofphosphatasesthroughsubstrateprofiling,PNAS,06.04.2015,Huang,etc.)，本申请中的酶在制备阿洛酮糖方面更有优势。技术方案本发明的一个目的是提供一种阿洛酮糖-6-磷酸磷酸酶。本发明的另一个目的是提供一种编码所述阿洛酮糖-6-磷酸磷酸酶的核酸。本发明的另一个目的是提供一种转化子，其包含编码阿洛酮糖-6-磷酸磷酸酶的核酸。本发明的又一个目的是提供一种用于制备阿洛酮糖的组合物，其包含阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物。本发明的又一个目的是提供一种制备阿洛酮糖的方法，其包括使阿洛酮糖-6-磷酸与阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物接触，以将阿洛酮糖-6-磷酸转化为阿洛酮糖。有益效果本发明的新型的酶及其组合是耐热的，因此，本发明的酶可以以工业方式参与将阿洛酮糖-6-磷酸转化为阿洛酮糖的途径，使得以葡萄糖或淀粉(例如麦芽糖糊精)为廉价原料合成阿洛酮糖的途径得以进行，并通过不可逆的反应途径，即阿洛酮糖-6-磷酸的去磷酸化，制备阿洛酮糖，从而显著提高阿洛酮糖的转化率。耐热酶可以经济地防止微生物，并且容易与高浓度底物发生反应，因此其效果显着。另外，通过提高阿洛酮糖的转化率，本发明的制备方法可以简化或消除分离及纯化的步骤(其中包含高浓度的反应产物(即阿洛酮糖))，因而本发明的制备方法具有经济优势，同时制备方法简单。特别地，可以减少或消除使用SMB进行的分离，从而最大限度地提高分离的效率和产量。具体实施方式在下文中将详细描述本发明。同时，对于常规元素，本发明公开的一方面和实施方案的描述都可以应用于其他方面和实施方案。此外，本发明公开的各种元素的所有组合都落入本发明的范围内。另外，本发明的范围不受限于以下的具体描述。为了实现上述的目的，本发明的一方面提供一种阿洛酮糖-6-磷酸磷酸酶。具体地，本发明的阿洛酮糖-6-磷酸磷酸酶可以为衍生自以下属的阿洛酮糖-6-磷酸磷酸酶：脂环酸芽孢杆菌属(Alicyclobacillus)、拟无枝酸菌属(Amycolatopsis)、厌氧绳菌属(Anaerolinea)、古球菌属(Archaeoglobus)、芽孢杆菌属(Bacillus)、热解纤维素果汁杆菌属(Caldicellulosiruptor)、暖绳菌属(Caldilinea)、暖发菌属(Caldithrix)、一氧化碳胞菌属(Carboxydocella)、一氧化碳嗜热菌属(Carboxydothermus)、绿弯菌门(Chloroflexi)、废水袍菌(Defluviitoga)、异常球菌属(Deinococcus)、除硫球菌属(Desulfurococcus)、网球菌属(Dictyoglomus)、Effusibacillus、闪烁杆菌属(Fervidobacterium)、地芽孢杆菌属(Geobacillus)、盐球菌属(Halococcus)、Hydrogenivirga、氢杆菌属(Hydrogenobacter)、超热菌属(Hyperthermus)、Kosmotoga、Marinitoga、亚栖热菌属(Meiothermus)、Mesotoga、生金球菌属(Metallosphaera)、甲烷胞菌属(Methanocella)、拟甲烷球菌属(Methanococcoides)、甲烷盐菌属(Methanohalobium)、甲烷叶菌属(Methanolobus)、甲烷八叠球菌属(Methanosarcina)、甲烷热菌属(Methanothermus)、石袍菌属(Petrotoga)、嗜酸菌属(Picrophilus)、假诺卡氏菌属(Pseudonocardia)、火球菌属(Pyrococcus)、热网菌属(Pyrodictium)、红嗜热盐菌属(Rhodothermus)、史雷克氏菌属(Slackia)、葡萄嗜热菌属(Staphylothermus)、硫化叶菌属(Sulfolobus)、Thermanaerothrix、Thermoanaerobacter、Thermoanaerobacterium、嗜热裂孢菌属(Thermobifida)、热球菌属(Thermococcus)、Thermocrinis,Thermoflexus、栖热袍菌属(Thermotoga)、栖热菌属(Thermus)以及特吕珀菌属(Truepera)，并且，更具体地，衍生自酸热脂环芽孢杆菌(Alicyclobacillusacidocaldarius)、腾冲脂环酸芽孢杆菌(Alicyclobacillustengchongensis)、热黄拟无枝酸菌(Amycolatopsisthermoflava)、Anaerolineathermolimosa、Anaerolineathermophila、Archaeoglobusfugidus、Archaeoglobusprofundus、嗜热硫酸盐还原菌(Archaeoglobusveneficus)、地衣芽孢杆菌(Bacilluslicheniformis)、热解纤维素菌(Caldicellulosiruptorbescii)、Caldilineaaerophila、Caldithrixabyssi、Carboxydocellasp.ULO1、Carboxydothermusferrireducens、绿弯菌门细菌54-19(Chloroflexibacterium54-19)、突尼斯废水袍菌(Defluviitogatunisiensis)、Deinococcusaerius、Deinococcusapachensis、Deinococcusaquatilis、Deinococcusgeothermalis、Deinococcushopiensis、Deinococcusmaricopensis、Deinococcusmurrayi、Deinococcusreticulitermitis、Deinococcuswulumuqiensis、Deinococcussp.Leaf326、Deinococcusphoenicis、Deinococcusproteolyticus,Deinococcussp.17bor-2、Deinococcussp.NW-56,Deinococcussp.RL、Deinococcussp.YIM77859、粘硫还原球菌(Desulfurococcusmucosus)、嗜热网球菌(Dictyoglomusturgidum)、Effusibacilluspohliae、Fervidobacteriumgondwanense、Fervidobacteriumislandicum、Fervidobacteriumnodosum、Fervidobacteriumpennivorans、Geobacillussp、嗜热脂肪地芽孢杆菌(Geobacillusstearothermophilus)、Halococcussalifodinae、Hydrogenivirgasp.128-5-R1-1、嗜氢产氢杆菌(Hydrogenobacterhydrogenophilus)、嗜氢氢杆菌(Hydrogenobacterthermophilus)、丁酸栖高温菌(Hyperthermusbutylicus)、Kosmotogaarenicorallina、Kosmotogaolearia、Marinitogapiezophila、Meiothermuscerbereus、Meiothermuschliarophilus、Meiothermusruber、MeiothermusSilvanus、Meiothermustaiwanensis、Meiothermustimidus、Meiothermusrufus、Mesotogainfera、Metallosphaerasedula、Methanocellaconradii、Methanococcoidesmethylutens、Methanohalobiumevestigatum、Methanolobustindarius、Methanosarcinasicilia、Methanothermusfervidus、Petrotogamobilis、Picrophilustorridus、Pseudonocardiathermophila、Pyrococcusfuriosus、Pyrodictiumoccultum、海洋红嗜热盐菌(Rhodothermusmarinus)、Slackiaheliotrinireducens、海葡萄嗜热菌(Staphylothermusmarinus)、嗜酸热硫化叶菌(Sulfolobusacidocaldarius)、Thermanaerothrixdaxensis、嗜热厌氧菌(Thermoanaerobactersp.)、热硫化氢嗜热厌氧杆菌(Thermoanaerobacterthermohydrosulfuricus)、威吉利热厌氧杆菌(Thermoanaerobacterwiegelii)、木聚糖热厌氧杆菌(Thermoanaerobacteriumxylanolyticum),耐盐嗜热双岐菌(Thermobifidahalotolerans)、速生热球菌(Thermococcusceler)、Thermococcuslitoralis、深嗜热球菌(Thermococcusprofundus)、密涅瓦热发状菌(Thermocrinisminervae)、Thermocrinisruber、Thermoflexushugenholtzii、莱廷格热袍菌(Thermotogalettingae)、那不勒斯栖热袍菌(Thermotoganeapolitana)、嗜石油热袍菌(Thermotogapetrophilia)、Thermusamyloliquefaciens、丝状栖热菌(Thermusfiliformis)、嗜热栖热菌(Thermusthermophilus)或Trueperaradiovictrix，但不限于此。如本文所用，已知术语“阿洛酮糖-6-磷酸(psicose-6-phosphate)”为阿洛酮糖-6-磷酸(allulose-6-phosphate)，并且所述“阿洛酮糖-6-磷酸磷酸酶”是指这样的酶，其通过将阿洛酮糖-6-磷酸的磷酸基团去磷酸化，催化阿洛酮糖的产生。在分解高浓度淀粉制备阿洛酮糖的过程中，可以使用本发明的阿洛酮糖-6-磷酸磷酸酶，与淀粉加工酶和磷酸糖-转化酶结合，以高效地制备阿洛酮糖。本发明的阿洛酮糖-6-磷酸磷酸酶可以具有SEQIDNO：1至222中的任一氨基酸序列，或包含与所述氨基酸序列具有至少70％的同源性的氨基酸序列。另外，其可以具有SEQIDNO：1、6、9、12、26、29、38至43、45至53、56、57、59、60、64至66、69、70、72、76、80、81、91至93、95、99至103、113、114、116、117、131、134、136、142、145、146、148、164、167、169、172、177、184至187、189、191、192、211、217和221中的任一氨基酸序列，或包含与所述氨基酸序列具有至少70％的同源性的氨基酸序列。更特别地，其可以具有SEQIDNO：26、29、53、56、60、70、76、80、81、116、117、131、134、145、167、185、186和191中的任一氨基酸序列，或包含与所述氨基酸序列具有至少70％的同源性的氨基酸序列，但不限于此。此外，可以包括但不限于具有与上述氨基酸序列相同活性的任何序列。另外，其可以包含SEQIDNO：1至222中的任一氨基酸序列，或包含与上述氨基酸序列具有至少80％的同源性(homology)或相同性(identity)的氨基酸序列，但不限于此。特别地，所述氨基酸可以包括与上述氨基酸序列具有至少70％、75％、80％、85％、90％、95％、96％、97％、98％或99％同源性或相同性的氨基酸。此外，具有如下的氨基酸序列的任何蛋白显然也可以落入本发明的范围内，只要其包含具有上述同源性或相同性的氨基酸序列，并表现出与上述蛋白相应的功能，其中所述氨基酸序列的部分缺失、修饰、取代或添加。即，在本发明中，尽管描述为“具有特定SEQIDNO的氨基酸序列的蛋白”，但是，如下的任何蛋白显然也可以用于本发明，只要所述蛋白具有与相应的SEQIDNO的氨基酸序列组成的蛋白相同或相应的活性，其中蛋白的氨基酸序列的部分缺失、修饰、取代或添加。例如，只要所述蛋白具有与所述酶相同或相应的活性，就不排除在其氨基酸序列的上游或下游的序列的添加(这不会改变蛋白的功能)、其自然发生的突变、沉默突变或保守取代。即使所述蛋白存在这样的序列添加或突变，其也显然落入本发明的范围内。如本文所用，术语“同源性”或“相同性”是指两个给定的氨基酸序列或核苷酸序列之间的相关程度，并且可以表示为百分比。术语“同源性”和“相同性”通常可以互换使用。保守的多核苷酸或多肽的序列同源性或相同性可以通过标准比对算法来确定，并且可以与所使用的程序所建立的默认空位罚分一起使用。基本上，在中等或高度严格条件下，同源或相同的序列可以杂交，使得序列的全长或全长的至少约50％、60％、70％、80％或90％或更多可以杂交。在杂交的多核苷酸中，还考虑包含简并密码子而非密码子的多核苷酸。任何两个多核苷酸序列是否具有同源性、相似性或相同性，例如可以通过已知的计算机算法例如使用默认参数的“FASTA”程序(Pearsonetal.,(1988)[Proc.Natl.Acad.Sci.USA85]:2444)来确定。或者，也可以通过Needleman-Wunsch算法(NeedlemanandWunsch,1970,J.MolBiol.48:443-453)来确定，其通过EMBOSS软件包的Needle程序运行(EMBOSS:欧洲分子生物学开放软件套件，Riceetal.,2000,TrendsGenet.16:276-277)(优选5.0.0或之后的版本)(GCG程序包)(Devereux,J.etal.,NucleicAcidsResearch12:387(1984)),BLASTP,BLASTN,FASTA(Atschul,S.F.etal.,JMOLECBIOL215:403(1990)；GuidetoHugeComputers,MartinJ.Bishop,ed.,AcademicPress,SanDiego,1994；andCARILLOetal.(1988)SIAMJAppliedMath48:1073)(1988)SIAMJAppliedMath48:1073)。例如，同源性、相似性或相同性可以使用美国国家生物技术信息中心(NCBI)的BLAST和ClustalW确定。多核苷酸或多肽的同源性、相似性或相同性可以通过使用例如GAP计算机程序比对序列信息来确定，例如在SmithandWaterman,Adv.Appl.Math(1981)2:482中公开的Needlemanetal.(1970),JMolBiol.48:443。总之，GAP程序将同源性、相似性或相同性定义为相似比对的符号(即核苷酸或氨基酸)的数目除以两个序列中较短者的符号的总数而获得的值。用于GAP程序的默认参数可以包括(1)一元比较矩阵(其包含的数值对于相同为1，对于不同为0)以及在SchwartzandDayhoff，eds.,AtlasOfProteinSequenceAndStructure,NationalBiomedicalResearchFoundation,pp.353-358(1979)(或EDNAFULL(NCBINUC4.4的EMBOSS版本)替换矩阵)中描述的Gribskovetal.(Nucl.AcidsRes.14:6745(1986))的加权比较矩阵；(2)每个空位罚分是3.0，每个空位的每个符号附加0.10罚分(或空位开位罚分10和空位扩展罚分0.5)；(3)末端空位不罚分。因此，如本文所用，术语“同源性”或“相同性”是指序列之间的相关性(relevance)。本发明的阿洛酮糖-6-磷酸磷酸酶可以是选择性催化阿洛酮糖-6-磷酸的去磷酸化的酶。具体地，与葡萄糖-1-磷酸(glucose-1-phosphate)、葡萄糖-6-磷酸(glucose-6-phosphate)、或果糖-6-磷酸(fructose-6-phosphate)混合在一起时，阿洛酮糖-6-磷酸磷酸酶可以是催化阿洛酮糖-6-磷酸的去磷酸化的酶。例如，当阿洛酮糖-6-磷酸磷酸酶与等量的阿洛酮糖-6-磷酸、葡萄糖-1-磷酸、葡萄糖-6-磷酸以及果糖-6-磷酸混合时，阿洛酮糖-6-磷酸的去磷酸化效率为1％或更高，10％或跟高，或30％或更高。由于本发明的阿洛酮糖-6-磷酸磷酸酶的选择性活性，在一锅法酶转化中，同时使用多种酶和底物表现出高效率的阿洛酮糖转化。本发明的阿洛酮糖-6-磷酸磷酸酶可以通过以下制备，用酶本身或表达所述酶的DNA来转化菌株，培养所转化的菌株以获得培养物，并破坏培养物，随后通过柱层析纯化。用于转化的菌株可以是大肠杆菌(Escherichiacoli)、谷氨酸棒杆菌(Corynebacterumglutamicum)、米曲霉(Aspergillusoryzae)、酿酒酵母(Saccharomycescerevisiae)、Yarrowialipolytica、毕赤酵母(Pichiapastoris)或枯草芽孢杆菌(Bacillussubtilis)，但不限于此，并且其可能有潜力随后被转化为GRAS菌株(通常被认为是安全的)。本发明的另一个方面提供一种编码阿洛酮糖-6-磷酸磷酸酶的核酸，或包含所述核酸的载体。如本文所用，术语“核酸”具有共同包括DNA或RNA分子的含义。作为核酸基本结构单元的核苷酸不仅包括天然核苷酸，还包括其中糖或碱基位点被修饰的类似物(参见Scheit,NucleotideAnalogs,JohnWiley,NewYork(1980)；UhlmanandPeyman,ChemicalReviews,90:543-584(1990))。本发明的编码所述酶的核酸可以是这样的DNA或RNA序列，其中作为单元的核苷酸共价连接，并且特别地，其可以是SEQIDNO：1至222(将氨基酸修饰为61个密码子)的氨基酸序列在DNA转化时所有可能的数字中的任一核苷酸序列，更特别地，其可以包括一种核酸，所述核酸能够被翻译以表现出所需的酶活性，同时与每个核苷酸具有至少90％或更高、95％或更高、97％或更高、99％或更高或100％同源性、相似性或相同性，所述核苷酸可以被翻译成本发明的SEQIDNO：1至222中的任一氨基酸序列。显然，由于密码子的简并性，具有相同活性的蛋白、转录后具有相同氨基酸序列的蛋白，特别地，由SEQIDNO：1至222中的任一氨基酸序列组成的蛋白，或可以翻译成与其具有同源性、相似性或相同性的蛋白的多核苷酸也落入本发明的范围内。更特别地，本发明所述的核酸序列并没有单独提出，其可以包含所有数字的DNA密码子，所述密码子可以被翻译成SEQIDNO：1至222的氨基酸序列，但不限于此。另外，可以根据可已知的基因序列制备的探针，例如在严格条件下可以与编码本发明所述的酶的核苷酸序列的全部或部分互补的序列杂交的任何序列，亦可无限制地包括在内。“严格条件(stringentcondition)”是指在该条件下多核苷酸之间可以特异性杂交的条件。这样的条件在文献(J.Sambrooketal.,supra9.50–9.51,11.7–11.8)中有具体的描述。例如，严格条件可以包括，在该条件下，具有高同源性或相同性的基因，即具有80％或更高，85％或更高，特别地，90％或更高，更特别地，95％或更高，更具体的97％或更高，更特别地99％或更高的同源性或相同性的基因彼此杂交，而同源性或相同性低于上述同源性或相同性的基因彼此不杂交；或者可以包括Southern杂交的清洗条件，即在60℃、1×SSC和0.1％SDS，特别地，60℃、0.1×SSC和0.1％SDS，并且特别地，68℃、0.1×SSC和0.1％SDS的盐浓度和温度下，清洗一次，特别地，清洗两次或三次的条件。杂交需要两个核酸具有互补序列，即使碱基之间可能错配(mismatch)，这取决于杂交的严格性。术语“互补的”用于描述能够彼此杂交的核苷酸碱基之间的关系。例如，对于DNA，腺嘌呤与胸腺嘧啶互补，胞嘧啶与鸟嘌呤互补。因此，本发明可以包括与整个序列互补的分离的核苷酸片段，以及与其基本相似的核酸序列。特别地，具有同源性或相同性的多核苷酸可以使用包括在上述条件下的Tm值为55℃的杂交步骤的杂交条件下来检测。另外，Tm值可以是60℃、63℃或65℃，但不限于此，并且本领域技术人员可以根据目的适当地调整。用于杂交多核苷酸的合适的严格性取决于多核苷酸的长度和互补程度，并且这些变量是本领域众所周知的(参见Sambrook等，同上文，9.50-9.51，11.7-11.8)。如本文所用，术语“载体”可以指DNA构建体，其包含编码本发明的酶的核酸的核苷酸序列，所述核苷酸序列被可操作性地连接到一个合适的调节序列上，以使经修饰的目标蛋白可以在合适的宿主中表达。所述调节序列可以包含能够启动转录的启动子，任何调节转录的操纵子序列，编码合适的mRNA核糖体结合结构域的序列，以及调节转录和翻译终止的序列。转化至合适的宿主细胞后，所述载体可以复制或者独立于宿主的基因组而发挥作用，并可以整合到宿主的基因组中。本发明使用的载体没有特别的限制，只要其能够在宿主细胞中复制，并且可以使用本领域已知的任何载体。常用的载体的实例可以包括天然或重组质粒、粘粒、病毒和噬菌体。例如，pWE15、M13、MBL3、MBL4、IXII、ASHII、APII、t10、t11、Charon4A和Charon21A等可用作噬菌体载体或粘粒载体，基于pBR、pUC、pBluescriptII、pGEM、pTZ、pCL和pET等的载体可用作质粒载体。特别地，可以使用pDZ、pACYC177、pACYC184、pCL、pECCG117、pUC19、pBR322、pMW118和pCC1BAC载体。本发明的又一方面提供一种转化子，其包含具有编码本发明的酶的核酸的载体。如本文所用，“包含编码所述酶的核酸的转化子”或者“包含具有编码所述酶的核酸的载体的转化子”可以指微生物，其经过重组以表达本发明的阿洛酮糖-6-磷酸磷酸酶。例如，其可以是宿主细胞或微生物，其含有编码阿洛酮糖-6-磷酸磷酸酶的核酸，或其通过含有编码阿洛酮糖-6-磷酸磷酸酶的核酸的载体进行转化，以表达阿洛酮糖-6-磷酸磷酸酶。为了本发明的目的，通过所述转化子表达的阿洛酮糖-6-磷酸磷酸酶可以包含SEQIDNO:1至222中的任一氨基酸序列，但不限于此。如本文所用，术语“转化”是指将包含编码阿洛酮糖-6-磷酸磷酸酶的核酸的载体引入到宿主细胞中，以使所述核酸编码的蛋白得以在宿主细胞中表达。转化的核酸是插入并位于宿主细胞的染色体中，或者是位于染色体外，或者是包括上述两种情况，这并不重要，只要其可以在宿主细胞中表达。此外，所述核酸可以包含编码核酸的DNA和RNA，所述DNA和RNA编码本发明的阿洛酮糖-6-磷酸磷酸酶。核酸可以任意形式引入，只要其可以被转入到宿主细胞，并在其中表达。例如，可以以表达盒(expressioncassette)的形式将核酸引入宿主细胞，所述表达盒为包含自主表达所需的所有元件的基因构建体。通常，表达盒可包含与核酸可操作地连接的启动子(promoter)、转录终止子、核糖体结合结构域或翻译终止子。表达盒可以是自复制的表达载体的形式。另外，可以将核酸本身引入宿主细胞，并与在宿主细胞中表达所需的序列可操作地连接，但不限于此。另外，如本文所用，术语“可操作地连接”是指基因序列功能性地连接至启动子序列，所述启动子序列启动和调节核酸的转录，所述核酸编码本发明的阿洛酮糖-6-磷酸磷酸酶。可以通过本领域已知的任何方法将核酸或载体插入到染色体中，例如，通过同源重组，但所述方法不限于此。另外，载体还可以包含选择标记以确认染色体的插入。选择标记用于选择通过载体转化的细胞，即用于确认目标核酸分子是否已插入；可以使用如下能够提供可选择的表型的标记，例如耐药性、营养缺陷型、对细胞毒剂的抗性，或表面修饰的蛋白的表达。当使用选择性试剂进行治疗时，仅表达选择标记的细胞可以存活或者表达其他表型性状，因此可以选择转化的细胞。本发明的转化载体的方法可以包括任何能将核酸引入细胞的方法，并且根据宿主细胞，可以选择本领域已知的合适标准技术实施。例如，所述方法可以包括电穿孔、磷酸钙(CaPO4)、氯化钙(CaCl2)沉淀、逆转录病毒感染、显微注射、聚乙二醇(PEG)法、DEAE-葡聚糖法、阳离子脂质体法和醋酸锂-DMSO法，但不限于此。对于宿主细胞，优选使用DNA引入效率高，并且高效率地表达引入的DNA的宿主细胞。例如，可以是Corynebacterium属微生物、Escherichia属微生物、Serratia属微生物、Bacillus属微生物、Saccharomyces属微生物或Pichia属微生物，并且特别地，可能是E.coli，但不限于此，并且可以应用于所有GRAS菌株。更特别地，本发明的转化子总共可以有222种，即E.coliBL21(DE3)/pET-CJ-ap1至E.coliBL21(DE3)/pET-CJ-ap222。本发明的另一方面提供一种用于制备阿洛酮糖的组合物，其包含阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物。特别地，所述组合物还可以包含磷酸糖。更特别地，所述组合物还可以包含阿洛酮糖-6-磷酸作为底物，但不限于此。本发明的用于制备阿洛酮糖的组合物可以通过包含使阿洛酮糖-6-磷酸去磷酸化的阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物，使阿洛酮糖-6-磷酸去磷酸化以制备阿洛酮糖。另外，本发明的用于制备阿洛酮糖的组合物还可以包含酶和/或底物[(i)淀粉、麦芽糖糊精、蔗糖或其组合；(ii)磷酸盐(phosphate)或聚磷酸盐(polyphosphate)或其他磷酸化化合物；(iii)果糖-6-磷酸-3-差向异构酶；(iv)葡萄糖-6-磷酸异构酶；(v)磷酸葡萄糖变位酶或葡萄糖激酶；和/或(vi)α-葡聚糖磷酸化酶、淀粉磷酸化酶、麦芽糖糊精磷酸化酶、蔗糖磷酸化酶、α-淀粉酶、支链淀粉酶、异淀粉酶、葡萄糖淀粉酶、α-葡聚糖转移酶、聚磷酸葡萄糖激酶或蔗糖酶]，其参与本发明的阿洛酮糖制备途径葡萄糖淀粉酶聚磷酸葡萄糖激酶；表达参与到阿洛酮糖制备途径的酶的微生物；或微生物的培养物，所述微生物表达参与到阿洛酮糖制备途径的酶。然而，本发明的用于制备阿洛酮糖的组合物中包含的酶，以及用于制备阿洛酮糖的底物仅为说明性，并不限于此，只要可以使用本发明的阿洛酮糖-6-磷酸磷酸酶制备阿洛酮糖。特别地，所述果糖-6-磷酸-3-差向异构酶可以包含任何在果糖-6-磷酸转化为阿洛酮糖-6-磷酸的过程中具有活性的蛋白。葡萄糖-6-磷酸异构酶可以包含任何在葡萄糖-6-磷酸转化为果糖-6-磷酸的过程中具有活性的蛋白。磷酸葡萄糖变位酶(phosphoglucomutase,EC5.4.2.2)可以包含任何在葡萄糖-1-磷酸转化为葡萄糖-6-磷酸的过程中具有活性的蛋白。淀粉/麦芽糖糊精磷酸化酶(starch/maltodextrinphosphorylase,EC2.4.1.1)和α-葡聚糖磷酸化酶可以包含任何在磷酰基转移到葡萄糖，以由淀粉或麦芽糖糊精制备葡萄糖-1-磷酸的过程中具有活性的蛋白。蔗糖磷酸化酶(sucrosephosphorylase,EC2.4.1.7)可以包含任何在磷酰基转移到葡萄糖，以从蔗糖制备葡萄糖-1-磷酸的过程中具有活性的蛋白。α-淀粉酶(α-amylase,EC3.2.1.1)、支链淀粉酶(pullulanase,EC3.2.1.41)、异淀粉酶(isoamylase,EC3.2.1.68)、4-α-葡聚糖转移酶(4-α-glucanotransferase,EC2.4.1.25)和葡萄糖淀粉酶(glucoamylase,EC3.2.1.3)是淀粉液化酶，其可以包含任何在淀粉或麦芽糊精转化为脱支麦芽低聚糖或葡萄糖的过程中具有活性的蛋白。蔗糖酶(sucrase,EC3.2.1.26)可以包含任何在蔗糖转化为葡萄糖的过程中具有活性的蛋白。聚磷酸葡萄糖激酶(polyphosphateglucokinase,EC2.7.1.63)可以包含任何在磷酰基转移至葡萄糖以将葡萄糖转化为葡萄糖-6-磷酸的过程中具有活性的蛋白。本发明的用于制备阿洛酮糖的组合物包括阿洛酮糖-6-磷酸磷酸酶、α-葡聚糖磷酸化酶、磷酸葡萄糖变位酶(或磷酸甘露糖变位酶)、葡萄糖-6-磷酸异构酶、阿洛酮糖-6-磷酸-3-差向异构酶(或核酮糖-5-磷酸-3-差向异构酶)、支链淀粉酶(或异淀粉酶)、4-α-葡聚糖转移酶和聚磷酸葡萄糖激酶，其与最终产物阿洛酮糖几乎没有或没有副反应。本发明的用于制备阿洛酮糖的组合物不仅可以适当地含有阿洛酮糖-6-磷酸磷酸酶，还可以适当地含有用于制备阿洛酮糖的多种酶及其底物。阿洛酮糖-6-磷酸磷酸酶具有如下的作用，即使处于存在多种酶的环境中，也能够由阿洛酮糖-6-磷酸选择性地且不可逆地制备阿洛酮糖。本发明的用于制备阿洛酮糖的组合物还可以包含任何合适的常规使用的赋形剂。这样的赋形剂的实例包括，例如，防腐剂、润湿剂、分散剂、悬浮剂、缓冲剂、稳定剂和等渗剂，但不限于此。本发明的用于制备阿洛酮糖的组合物可以进一步包含金属离子或金属盐。在一实施方案中，金属离子可以是二价金属阳离子。特别地，金属离子可以为选自以下的至少一种：Ni、Mg、Co、Mn、Fe和Zn离子。更特别地，本发明用于制备塔格糖的组合物可以进一步包含金属盐。更特别地，金属盐可以为选自以下的至少一种：NiSO4、MgSO4、MgCl2、NiCl2、CoSO4、CoCl2、MnCl2、MnSO4、FeSO4和ZnSO4。本发明的另一个方面提供了一种制备阿洛酮糖的方法，其包括以下步骤，通过使阿洛酮糖-6-磷酸与阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物接触，以将阿洛酮糖-6-磷酸转化为阿洛酮糖。特别地，在制备阿洛酮糖的方法中，可以通过以下方法制备阿洛酮糖，使阿洛酮糖-6-磷酸与阿洛酮糖-6-磷酸磷酸酶、表达所述酶的微生物或所述微生物的培养物接触，但不限于此。本发明的方法还可以包括在将阿洛酮糖-6-磷酸转化为阿洛酮糖之前，通过使果糖-6-磷酸与果糖-6-磷酸-3-差向异构酶、表达果糖-6-磷酸-3-差向异构酶的微生物或表达果糖-6-磷酸-3-差向异构酶的微生物的培养物接触，以将果糖-6-磷酸转化为阿洛酮糖-6-磷酸。本发明的方法还可以包括在将果糖-6-磷酸转化为阿洛酮糖-6-磷酸之前，通过使葡萄糖-6-磷酸与葡萄糖-6-磷酸异构酶、表达葡萄糖-6-磷酸异构酶的微生物或表达葡萄糖-6-磷酸异构酶的微生物的培养物接触，以将葡萄糖-6-磷酸转化为果糖-6-磷酸。本发明的方法还可以包括在将葡萄糖-6-磷酸转化为果糖-6-磷酸之前，通过使葡萄糖-1-磷酸与磷酸葡萄糖变位酶、表达磷酸葡萄糖变位酶的微生物或表达磷酸葡萄糖变位酶的微生物的培养物接触，以将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸。本发明的方法还可以包括在将葡萄糖-6-磷酸转化为果糖-6-磷酸之前，通过使葡萄糖与聚磷酸葡萄糖激酶、表达聚磷酸葡萄糖激酶的微生物或表达聚磷酸葡萄糖激酶的微生物的培养物以及聚磷酸盐接触，以将葡萄糖转化为葡萄糖-6-磷酸。本发明的方法还可以包括在将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸之前，通过使淀粉、麦芽糊精、蔗糖或其组合与α-葡聚糖磷酸化酶、淀粉磷酸化酶、麦芽糊精磷酸化酶或蔗糖磷酸化酶；表达所述磷酸化酶的微生物或表达所述磷酸化酶的微生物的培养物以及磷酸盐接触，以将淀粉、麦芽糊精、蔗糖或其组合转化为葡萄糖-1-磷酸。本发明的方法还可以包括在将淀粉、麦芽糖糊精、蔗糖或其组合转化为葡萄糖-1-磷酸之前，通过使淀粉、麦芽糊精、蔗糖或其组合与α-淀粉酶、支链淀粉酶、异淀粉酶、葡萄糖淀粉酶或蔗糖酶；表达所述α-淀粉酶、支链淀粉酶、异淀粉酶、葡萄糖淀粉酶或蔗糖酶的微生物或表达所述α-淀粉酶、支链淀粉酶、葡萄糖淀粉酶、蔗糖酶或异淀粉酶的微生物的培养物接触，以将淀粉、麦芽糊精、蔗糖或其组合转化为葡萄糖。本发明的制备阿洛酮糖的方法中使用的阿洛酮糖-6-磷酸磷酸酶、α-葡聚糖磷酸化酶、磷酸葡萄糖变位酶(或磷酸甘露糖变位酶)、葡萄糖-6-磷酸异构酶、阿洛酮糖-6-磷酸-3-差向异构酶(或核酮糖-5-磷酸-3-差向异构酶)、支链淀粉酶(或异淀粉酶)、4-α-葡聚糖转移酶以及聚磷酸葡萄糖激酶，与最终产物阿洛酮糖几乎没有或没有副反应。在发明的制备阿洛酮糖的方法中，可以通过在磷酸糖-转化酶的复杂组合物中分解高浓度的淀粉，制备最优/最大量的阿洛酮糖，并且可以最多使用8种酶，以确保阿洛酮糖的最大产量。首先，降解淀粉并产生葡萄糖-1-磷酸的酶，即葡聚糖磷酸化酶(糖原磷酸化酶)(glucanphosphorylase,glycogenphosphorylase,EC2.4.1.1)，利用α-1,4-淀粉特异性地制备葡萄糖-1-磷酸。其次，在中间复合酶反应中，使用磷酸葡萄糖变位酶(phosphoglucomutase,EC2.7.5.1)或磷酸甘露糖变位酶(phosphomannomutase,EC5.4.2.8)，将由此产生的葡萄糖-1-磷酸转化为葡萄糖-6-磷酸。使用的第三种酶，即葡萄糖-6-磷酸异构酶(glucose-6-phosphateisomerase,EC5.3.1.9)，将葡萄糖-6-磷酸转化为果糖-6-磷酸。第四，使用核酮糖-5-磷酸-3-差向异构酶或阿洛酮糖-6-磷酸-3-差向异构酶，即如上所述将果糖-6-磷酸转化为阿洛酮糖-6-磷酸的酶，在可逆反应中制备阿洛酮糖-6-磷酸。在转化淀粉至阿洛酮糖-6-磷酸的反应中，虽然在可逆反应中的制备不能够超过一定的量，但使用本发明的选择性阿洛酮糖-6-磷酸磷酸酶能够实现阿洛酮糖的高产量。为进一步提高淀粉利用率，一起使用支链淀粉酶(EC3.2.1.41)或异淀粉酶(EC3.2.1.68)，除了支链淀粉的α-1,4键，还降解支链淀粉的支化的α-1,6键。此外，为了提高葡聚糖磷酸化酶的淀粉利用率，使用4-α-葡聚糖转移酶(EC2.4.1.25)。通过将α-1,4键形式的低聚糖与麦芽糖或其他低聚糖(活性相对较低的底物)结合，可提升分段淀粉底物的利用率。此外，通过复杂酶促反应，聚磷酸-葡萄糖磷酸转移酶(EC2.7.1.63)可用于由淀粉使用后降解的葡萄糖制备额外的阿洛酮糖，从而确保最大的阿洛酮糖转化率。此外，在发明的方法中，所述接触可以在5.0至9.0的pH下进行，特别地，在6.0至8.0的pH下进行。在本发明的方法中，所述接触可以在40℃至80℃的温度下进行，特别地，在40℃至60℃或50℃至60℃的温度下进行。在本发明的方法中，所述接触可以进行2小时至24小时，特别地，6至24小时或120小时。在本发明的方法中，所述接触可以在5.0至9.0的pH，40℃至80℃的温度下，和/或进行2小时至24小时。特别地，所述接触可以在6.0至8.0的pH，40℃至60℃或50℃至60℃的温度下，进行6小时至24小时或120小时。本发明的方法还可以包括纯化阿洛酮糖。对于纯化阿洛酮糖的方法没有特别的限制，可以使用本领域已知的任何合适的方法。所述纯化方法的非限制性实例包括色谱法、分步结晶法和离子纯化法，可以单独实施或以其两种或更多种的组合实施。例如，可以通过色谱法纯化反应产物阿洛酮糖。在这种情况下，通过色谱法纯化糖类可以基于需要纯化的糖类与附着在离子树脂上的金属离子之间的结合力的微小差异来进行。本发明所述方法还可包括在纯化步骤之前或之后的漂白、去矿化，或两者皆有。所述漂白和/或去矿化可以制备更纯的阿洛酮糖，并且不含杂质。实施例以下将通过实施例更详细地描述本发明。然而，仅出于示例性目的以帮助理解本发明而提供这些实施例，并且本发明的范围不意图受这些实施例所限制。在本发明中，氨基酸可以表示为以下缩写或氨基酸名称。表1氨基酸类型缩写编码对应氨基酸的DNA密码子编码对应氨基酸的RNA密码子丙氨酸AGCT,GCC,GCA,GCGGCU,GCC,GCA,GCG精氨酸RAGA,AGGAGA,AGG天冬酰胺NAAT,AACAAU,AAC天冬氨酸DGAT,GACGAU,GAC半胱氨酸CTGT,TGCUGU,UGC谷氨酸EGAA,GAGGAA,GAG谷氨酰胺QCAA,CAGCAA,CAG甘氨酸GGGT,GGC,GGA,GGGGGU,GGC,GGA,GGG组氨酸HCAC,CATCAC,CAU异亮氨酸IATT,ATC,ATAAUU,AUC,AUA亮氨酸LTTA,TTG,CTT,CTC,CTA,CTGUUA,UUG,CUU,CUC,CUA,CUG赖氨酸KAAA,AAGAAA,AAG蛋氨酸MATGAUG苯丙氨酸FTTT,TTCUUU,UUC脯氨酸PCCT,CCC,CCA,CCGCCU,CCC,CCA,CCG丝氨酸STCT,TCC,TCA,TCGUCU,UCC,UCA,UCG苏氨酸TACT,ACC,ACA,ACGACU,ACC,ACA,ACG色氨酸WTGGUGG酪氨酸YTAT,TACUAU,UAC缬氨酸VGTT,GTC,GTA,GTGGUU,GUC,GUA,GUG实施例1：每种酶和转化的微生物的重组表达载体的制备为了提供用于制备阿洛酮糖的途径的必需的酶，选择耐热性基因，如下表2所示。表2使用每个培养菌株的基因合成或染色体DNA(基因组DNA)，通过聚合酶链反应(PCR)，对所选择氨基酸的基因进行扩增，并使用限制酶NdeI以及XhoI或SalI，将扩增的DNA插入质粒载体pET2la(Novagen)，以构建重组表达载体，所述质粒载体pET2la用于大肠杆菌表达。使用通用转化技术(参见Sambrooketal.1989)将表达载体转化到大肠杆菌BL21(DE3)菌株中，以制备的转化的微生物。特别地，所述SEQIDNO:26、29、53、56、60、70、76、80、81、116、117、131、134、145、167、185、186和191的阿洛酮糖-6-磷酸磷酸酶被转化到大肠杆菌BL21(DE3)菌株中，以制备转化的微生物，其被命名为E.coliBL21(DE3)/pET-CJ-ap26、E.coliBL21(DE3)/pET-CJ-ap29、E.coliBL21(DE3)/pET-CJ-ap53、E.coliBL21(DE3)/pET-CJ-ap56、E.coliBL21(DE3)/pET-CJ-ap60、E.coliBL21(DE3)/pET-CJ-ap70、E.coliBL21(DE3)/pET-CJ-ap76、E.coliBL21(DE3)/pET-CJ-ap80、E.coliBL21(DE3)/pET-CJ-ap81、E.coliBL21(DE3)/pET-CJ-ap116、E.coliBL21(DE3)/pET-CJ-ap117、E.coliBL21(DE3)/pET-CJ-ap131、E.coliBL21(DE3)/pET-CJ-ap134、E.coliBL21(DE3)/pET-CJ-ap145、E.coliBL21(DE3)/pET-CJ-ap167、E.coliBL21(DE3)/pET-CJ-ap185、E.coliBL21(DE3)/pET-CJ-ap186以及E.coliBL21(DE3)/pET-CJ-ap191。如此制备的转化的微生物于2018年11月14日被保藏于国际保藏机构韩国微生物保藏中心(KCCM)，保藏号为KCCM12390P(E.coliBL21(DE3)/pET-CJ-ap26)、KCCM12391P(E.coliBL21(DE3)/pET-CJ-ap29)、KCCM12392P(E.coliBL21(DE3)/pET-CJ-ap53)、KCCM12393P(E.coliBL21(DE3)/pET-CJ-ap56)、KCCM12394P(E.coliBL21(DE3)/pET-CJ-ap60)、KCCM12395P(E.coliBL21(DE3)/pET-CJ-ap70)、KCCM12396P(E.coliBL21(DE3)/pET-CJ-ap76)、KCCM12397P(E.coliBL21(DE3)/pET-CJ-ap80)、KCCM12398P(E.coliBL21(DE3)/pET-CJ-ap81)、KCCM12399P(E.coliBL21(DE3)/pET-CJ-ap116)、KCCM12400P(E.coliBL21(DE3)/pET-CJ-ap117)、KCCM12401P(E.coliBL21(DE3)/pET-CJ-ap131)、KCCM12402P(E.coliBL21(DE3)/pET-CJ-ap134)、KCCM12403P(E.coliBL21(DE3)/pET-CJ-ap145)、KCCM12404P(E.coliBL21(DE3)/pET-CJ-ap167)、KCCM12405P(E.coliBL21(DE3)/pET-CJ-ap185)、KCCM12406P(E.coliBL21(DE3)/pET-CJ-ap186)以及KCCM12407P(E.coliBL21(DE3)/pET-CJ-ap191)。实施例2：重组酶的制备为了制备重组酶，将实施例1中制备的每种转化微生物接种到含有5mLLB液体培养基的培养管中。在37℃的振荡培养箱中进行种培养，直至在600nm的吸光度达到2.0。将通过种培养获得的培养液接种在含有LB液体培养基的培养瓶中，然后进行主培养。当培养物在600nm的吸光度达到2.0时，加入1mMIPTG以诱导重组酶的表达和制备。在180rpm的搅拌速度和37℃的条件下进行培养。所述培养液在8000xg以及4℃下离心20分钟，以回收细胞。回收的细胞用50mMTris-HCl(pH8.0)缓冲液洗涤两次，并在同样的缓冲液中重悬，使用超声均质器破碎所述细胞。所述细胞裂解液在13000xg以及4℃下离心20分钟，以收取上清液。通过His-tag亲和层析，从上清液中纯化所述重组酶，用50mMTris-HCl缓冲液(pH8.0)透析所述纯化后的重组酶，并用于接下来的反应。实施例3：磷酸糖和阿洛酮糖-6-磷酸的脱磷酸活性分析以果糖-6-磷酸制备阿洛酮糖-6-磷酸后，确定通过阿洛酮糖-6-磷酸磷酸酶制备阿洛酮糖的活性，或者通过进一步加入三种酶，使用葡萄糖-1-磷酸为第一底物，通过检测阿洛酮糖来确定所述活性。特别地，对葡萄糖、果糖和阿洛酮糖进行定性和定量评估，其为通过将本发明的222种磷酸酶中的每一种与三种酶(即磷酸葡萄糖变位酶(EC2.7.5.1)或磷酸甘露糖变位酶(EC5.4.2.8)，其将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸；葡萄糖-6-磷酸异构酶(EC5.3.1.9)，其将葡萄糖-6-磷酸转化为果糖-6-磷酸；以及核酮糖-5-磷酸-3-差向异构酶或阿洛酮糖-6-磷酸-3-差向异构酶，其将果糖-6-磷酸转化为阿洛酮糖-6-磷酸)混合制备而成的常见的糖类。特别地，将50mM果糖-6-磷酸或20mM葡萄糖-1-磷酸悬浮于50mMTris-HCl(pH7.0)、50mM磷酸钠(pH6至7)或50mM磷酸钾(pH6至7)中，然后向其中加入0.1单位/mL的磷酸葡萄糖变位酶；磷酸甘露糖变位酶、葡萄糖-6-磷酸异构酶和核酮糖-5-磷酸-3-差向异构酶；或向其中加入阿洛酮糖-6-磷酸-3-差向异构酶以及实施例2中制备的222种重组阿洛酮糖-6-磷酸磷酸酶的每一种，混合物在45℃至70℃下反应1至24小时。使用SP0810色谱柱(Shodex)以及AminexHPX-87C色谱柱(Bio-RAD)，在80℃以及0.6mL/min的流动相流速下，使用折光率检测器(RID)进行检测，通过HPLC确认葡萄糖、果糖和阿洛酮糖的产生。之后，在制备的葡萄糖-1-磷酸、葡萄糖-6-磷酸、果糖-6-磷酸和阿洛酮糖-6-磷酸的混合溶液中，测定本发明的阿洛酮糖-6-磷酸磷酸酶的去磷酸化活性以及阿洛酮糖-6-磷酸的特异性(选择性)去磷酸化率。特别地，将0.1单位/mL的每种阿洛酮糖-6-磷酸磷酸酶以及5mMMgCl2(或MgSO4)加入到1％(w/v)葡萄糖-6-磷酸、葡萄糖-1-磷酸、果糖-6-磷酸和阿洛酮糖-6-磷酸的混合溶液中，混合物在50℃下反应12小时。使用AminexHPX-87C柱(Bio-RAD)，在80℃下以0.6mL/min的流动相流速，通过HPLC分析反应产物。用折光率检测器检测阿洛酮糖和其他糖类(果糖和葡萄糖)的产生。结果，在所有222种酶中，69种酶对阿洛酮糖-6-磷酸显示出高选择性的去磷酸化活性，45种酶对阿洛酮糖-6-磷酸显示出弱选择性的去磷酸化活性。此外，在108种酶中，无法确认选择性阿洛酮糖-6-磷酸的去磷酸化效价。然而，除去222种酶中的一种，证实了221种酶的去磷酸化活性，其中113种酶表现出高去磷酸化活性，108种酶表现出低去磷酸化活性。同时，具有SEQIDNO：54序列的酶被证实没有去磷酸化效价，其为已知的去磷酸化酶，但却被证实没有实质性的去磷酸化活性。结果如下表3总结。A＝低活性(由磷酸糖到单糖的去磷酸转化率为1-30％)B＝高活性(由磷酸糖到单糖的去磷酸转化率为30％或更高)C＝无活性(由磷酸糖到单糖的去磷酸转化率小于1％)表3实施例4：通过多重酶促反应(多重酶)分析产生阿洛酮糖的活性为了由麦芽糖糊精制备阿洛酮糖，选择了七种酶并同时反应(一锅法)。七种酶如下，葡聚糖磷酸化酶，其降解淀粉并将其转化为葡萄糖-1-磷酸，支链淀粉酶，其使淀粉脱支，4-α-葡聚糖转移酶，其增加淀粉底物的利用率，磷酸葡萄糖变位酶，其将葡萄糖-1-磷酸转化为葡萄糖-6-磷酸，葡萄糖-6-磷酸异构酶，其将葡萄糖-6-磷酸转化为果糖-6-磷酸，阿洛酮糖-6-磷酸-3-差向异构酶，其将果糖-6-磷酸转化为阿洛酮糖-6-磷酸，以及SEQIDNO：1至222的酶，其能够由阿洛酮糖-6-磷酸制备阿洛酮糖，制备上述七种酶，并以0.1单位/mL的量使用。将5％(w/v)麦芽糖糊精加入1mM至5mMMgCl2以及10mM至50mM磷酸钠(pH7.0)中，并使混合物在50℃的温度下反应12小时。使用AminexHPX-87C柱(Bio-RAD)，在80℃下以0.6mL/min的流动相流速，通过HPLC分析反应产物，并使用折光率检测器检测。结果，通过多重酶促反应证实了有麦芽糖糊精制备了阿洛酮糖。虽然已经参考特定的说明性实施例描述了本申请，但是本申请所属领域的技术人员将理解，在不脱离本发明技术精神或本质的情况下，本发明可以以其他特定形式实施。因此，应当认为所描述的实施方案在所有方面都仅仅是示例性的而非限制性的。此外，本发明的范围由所附的权利要求而非前文的具体描述指明。落入权利要求的等同物的含义和范围内的所有修改或变化都将包含在本发明的范围内。[保藏号]保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12390P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12391P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12392P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12393P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12394P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12395P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12396P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12397P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12398P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12399P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12400P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12401P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12402P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12403P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12404P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12405P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12406P保藏日期：2018年11月14日保藏单位：KCCM韩国微生物保藏中心登记号：KCCM12407P保藏日期：2018年11月14日<110>CJ第一制糖株式会社<120>新阿洛酮糖-6-磷酸酶，包含其制备阿洛酮糖的组合物，和用其制备阿洛酮糖的方法<130>OPA19273<150>KR10-2018-0159351<151>2018-12-11<160>222<170>KoPatentIn3.0<210>1<211>265<212>PRT<213>酸热脂环芽孢杆菌(Alicyclobacillusacidocaldarius)<400>1MetAspLysAlaTrpLeuAspGlyLeuAlaGluValValArgGluAla151015GlyArgLeuValGluGluIleAlaArgGlnGlyPheAspThrGlnPhe202530LysHisProGluGluArgArgAspProValThrThrAlaAspLeuAla354045CysAspAlaPheLeuLysGluArgLeuLeuThrLeuLeuProGluAla505560GlyTrpLeuSerGluGluThrLysAspArgProAspArgLeuGluLys65707580ArgTrpValTrpIleValAspProIleAspGlyThrArgGluPheVal859095ArgArgIleProGluTyrAlaIleSerValAlaLeuAlaArgAspGly100105110GluProValAlaGlyAlaValValAsnProAlaThrGlyAspLeuPhe115120125LeuGlyAlaValGlyValGlyAlaTrpArgAsnGlyThrProMetVal130135140CysSerArgIleArgGlyGluArgLeuThrIleLeuGlySerArgSer145150155160GluMetAsnArgGlyGluPheGluProPheAlaGlyIleLeuGluVal165170175ArgAlaValGlySerIleAlaTyrLysLeuAlaLeuValAlaAlaGly180185190GluAlaAspGlyThrPheSerLeuGlyProLysHisGluTrpAspIle195200205AlaAlaGlyValAlaLeuValLeuAlaAlaGlyGlyArgValHisAsp210215220GlyAlaGlyArgProPheArgPheAsnGlnProHisThrLeuThrArg225230235240GlyIleValAlaAlaThrArgGluAlaTyrGlyAspLeuAlaLeuLeu245250255IleGluArgHisAlaProArgArgAla260265<210>2<211>262<212>PRT<213>腾冲脂环酸芽孢杆菌(Alicyclobacillustengchongensis)<400>2MetAsnGlnAspValTyrThrArgLeuAlaAsnCysAsnLeuPheIle151015PheAspLeuAspGlyThrValTyrAlaGluThrAspHisPheGluHis202530TyrLeuGlnGlnLeuAlaArgPheValGlyAspGluAlaArgGluAla354045PheLeuArgGluAlaGlnGluSerLeuAlaGluGlnGlyGlyArgPhe505560TyrGlyGlnAlaPheThrArgLeuThrGlyGluProValAlaValGlu65707580AspGluProSerGluGlnHisLeuHisValAspAspProTrpGlyVal859095LeuGlnAlaValAlaAlaArgHisGlyValSerValThrAspArgAsp100105110LysAlaPheLeuArgThrArgAspPheMetAlaGlyProPheProMet115120125ThrProLeuAlaGlyLeuArgAspAlaMetLeuAlaLeuArgSerGlu130135140GlyArgHisValValLeuAlaThrAsnSerProGluProAspSerArg145150155160AlaIleLeuArgLysLeuGluLeuGluAspValLeuGluAspTyrVal165170175PheGlnAlaArgLysProTyrArgThrAlaGluHisPheThrArgTrp180185190LeuAlaHisTyrAspIleProProGluGlnAlaValSerValGlyAsp195200205HisTyrArgAsnGluMetArgProAlaIleArgLeuGlyMetAsnThr210215220IleTyrIleAspArgTyrIleGlyGlnProArgAlaAspValThrVal225230235240GlnLeuArgHisProGlyGluLeuAlaAspValLeuArgArgSerLeu245250255ArgSerAlaAlaAlaGlu260<210>3<211>218<212>PRT<213>腾冲脂环酸芽孢杆菌(Alicyclobacillustengchongensis)<400>3MetArgGlyIleValPheAspPheAspGlyThrLeuIleAspThrGlu151015SerAlaTrpTyrThrAlaPheCysAspLeuTyrGlnGlnHisArgVal202530GluLeuProLeuSerLeuTyrAlaGlnCysIleGlyThrThrHisAsp354045AlaPheAspProValAlaHisLeuIleAlaSerAlaGluMetProIle505560ThrGluSerGluIleGluGlnPheIleGlnArgArgHisHisGluLeu65707580MetArgGluLysSerLeuArgProGlyValLysLysThrIleGluThr859095AlaArgThrLeuGlyLeuArgIleGlyLeuAlaThrSerSerHisArg100105110AlaTrpIleAspProPheLeuGluArgLeuGlyIleAlaAspAlaPhe115120125HisCysIleArgThrAlaAspAspValLysArgValLysProAspPro130135140AlaLeuTyrGluLeuThrLeuAlaCysLeuGlyLeuLysProAspGlu145150155160AlaValAlaIleGluAspSerProAsnGlyAlaLeuAlaAlaValArg165170175AlaGlyMetHisValLeuCysThrProAsnAspValThrArgGlnLeu180185190ProPheProGluAsnCysAsnHisValSerSerLeuAspGluIleAsp195200205TrpProThrTrpLeuGlnGluArgAsnArg210215<210>4<211>271<212>PRT<213>热黄拟无枝酸菌(Amycolatopsisthermoflava)<400>4MetGluLysProLeuLeuIleAlaSerAspValAspGlyThrLeuLeu151015AspProMetGluArgMetSerProArgThrAlaAlaValLeuArgArg202530AlaThrAspAlaGlyIleProValValLeuAlaThrGlyArgProPro354045ArgTrpIleProSerValAlaGlnAlaGlyGlyLeuThrGlyTyrAla505560ValCysAlaAsnGlyAlaValLeuTyrAspIleGlyAlaAspGluVal65707580ValGlyValHisGlyAlaLeuGluProMetMetLeuAsnAspAlaVal859095HisGluLeuThrLysAlaLeuProGlyAlaGlyPheAlaAlaGluArg100105110ValGlyArgArgAlaLeuAspProAspValGluHisLeuValValGlu115120125HisGlyTyrHisAsnProTrpGlyAspGlyGluGlyValProValSer130135140ArgAlaGluIleValGlyArgProAlaValLysLeuLeuValSerHis145150155160ProAspMetThrSerAspGluMetAlaArgAlaAlaArgAlaValLeu165170175AspGluSerValAspIleThrPheSerAlaSerGlyGlyLeuIleGlu180185190IleAlaAlaHisGlyIleThrLysAlaThrGlyLeuAlaGluValAla195200205GluArgPheGlyValProValGluArgIleIleAlaPheGlyAspMet210215220ProAsnAspLeuGluMetLeuLysTrpAlaGlyHisGlyValAlaMet225230235240AlaAsnAlaHisProAspValLeuAlaIleAlaAspGluValThrSer245250255ProAsnSerGluAspGlyValAlaGlnValLeuGluArgTrpPhe260265270<210>5<211>220<212>PRT<213>热黄拟无枝酸菌(Amycolatopsisthermoflava)<400>5MetAlaLysValLeuValLeuAspValAspGlyThrLeuValAspThr151015AsnTyrHisHisAlaLeuAlaTrpPheArgAlaPheArgGlyHisGly202530ValThrValProValTrpArgLeuHisArgAlaIleGlyMetGlyGly354045AspGlnLeuValProGluValAlaGlyGlnGluValGluAspSerCys505560GlyAspAspValArgAlaLysTrpLysGluLeuValAspGlyMetLeu65707580ProGluValCysAlaLeuAspGlyAlaHisGluLeuLeuGlnAlaAla859095AspAspAlaGlyTyrArgValValLeuAlaSerSerGlyLysProAsp100105110HisValAspHisTyrLeuAspLeuIleAspGlyArgGluLeuAlaAla115120125AspTrpThrSerSerLysAspValGluAlaThrLysProGluProAsp130135140LeuLeuGluValAlaLeuGluLysValArgGlyGluGluAlaValVal145150155160IleGlyAspSerValTrpAspCysValAlaAlaArgArgIleGlyLeu165170175ProCysValGlyLeuLeuThrGlyGlyPheSerAlaAlaGluLeuThr180185190AspAlaGlyAlaAlaArgThrPheThrAspLeuArgGluLeuArgAla195200205SerLeuAspGluLeuProPheGlyAlaSerProArg210215220<210>6<211>406<212>PRT<213>Anaerolineathermolimosa<400>6MetValLysArgGluValLeuAlaIleIleProAlaArgGlyGlySer151015LysGlyIleProArgLysAsnValArgLeuPheAlaGlyHisProLeu202530IleAlaTyrSerIleAlaAlaAlaArgGlnSerGluLeuValThrArg354045ValIleValSerThrAspAspGluGluIleAlaArgValAlaArgAsp505560TyrGlyAlaGluThrProPheLeuArgProAlaGluLeuAlaGlnAsp65707580GlnThrLeuAspLeuProValPheGlnHisAlaLeuArgTrpLeuGlu859095GluGlnGluGlyTyrGlnProGluLeuValValGlnLeuArgProThr100105110SerProValArgProProAlaLeuValAspGluAlaValGlnIleLeu115120125LeuAlaHisProGluAlaHisSerValArgGlyValValProAlaGly130135140GlnAsnProHisLysMetTrpArgIleAspProGlnAsnGlyGlnMet145150155160IleProLeuLeuGlnValProGlyLeuGluGluProTyrAsnAlaPro165170175ArgGlnValLeuProProValTyrTrpGlnThrGlyHisIleAspVal180185190IleArgProGluValIleArgLysGlySerMetSerGlyGlnValIle195200205LeuProValMetValAspProAlaTyrThrValAspIleAspThrPro210215220ArgAspTrpAlaArgSerGluTrpLeuValTrpTyrSerGluLeuGlu225230235240IleValTyrProGlyHisArgArgArgProLeuProGluLysValAsp245250255LeuValValPheAspPheAspGlyValLeuThrAspAsnArgValTrp260265270ValAspGluGluGlyHisGluMetValAlaAlaAsnArgSerAspSer275280285LeuGlyValAlaTyrLeuArgArgSerGlyLeuLysMetValValLeu290295300SerThrGluThrAsnProValValAlaAlaArgCysArgLysMetGln305310315320IleProValValGlnGlyValTrpAspLysAlaGlyGluLeuProArg325330335IleMetAlaGluPheAsnAlaAlaProGluArgThrIleTyrValGly340345350AsnAspValAsnAspValProCysPheSerLeuValGlyCysAlaVal355360365AlaValAlaAspAlaGlnProAlaAlaArgArgAlaAlaAspIleVal370375380LeuSerArgAsnGlyGlyHisGlyAlaValArgGluLeuLeuAspIle385390395400LeuMetGlnArgMetLys405<210>7<211>226<212>PRT<213>Anaerolineathermolimosa<400>7MetGlyIleAspArgGlnArgValCysGlyIleCysPheAspValAsp151015GlyThrLeuSerAspThrAspAspLeuTrpValAlaArgLeuSerGly202530TrpLeuLysProValSerTrpIlePheProGlnArgAsnProSerArg354045PheAlaArgTrpAlaIleMetAlaAlaGluSerProGlyAsnLeuVal505560TyrSerLeuPheAspArgPheGlyLeuAspAspGluIleGlyArgLeu65707580PheHisTrpLeuAsnArgGlnArgGlyValLeuArgSerGlnArgPhe859095LeuLeuIleProGlyValGlnGluAlaLeuHisArgLeuHisLysHis100105110PheProLeuAlaValValSerAlaArgAspHisAlaGlyThrLeuAla115120125PheLeuGlnHisPheGluLeuGluProPhePheArgCysValAlaThr130135140SerGlnThrCysGluPheThrLysProPheProHisProIleArgTrp145150155160AlaAlaAlaGlnMetGlyValLysProGluGluLeuLeuMetValGly165170175AspThrValValAspIleArgAlaGlyLysAlaAlaGlyAlaGlnThr180185190ValGlyValLeuCysGlyPheGlyGlnGluGluGluLeuArgArgAla195200205GlyAlaAspLeuIleLeuProHisThrAlaGlnLeuAlaAspValLeu210215220LeuAsp225<210>8<211>222<212>PRT<213>Anaerolineathermolimosa<400>8MetHisPheSerAlaTyrIlePheAspMetAspGlyThrLeuIleAsp151015AsnMetGlyPheHisGlyAlaIleTrpSerGluPheLeuAlaSerLeu202530GlyAlaProValAspArgGluThrPhePheArgArgThrValGlyLys354045ValAsnAlaGluIleLeuArgAspLeuTyrArgAlaAspLeuSerAsp505560AlaGluIleGluGluLeuSerArgArgLysGluGlnLeuTyrArgGln65707580ArgPheAlaProLeuIleThrGlnLysAlaValProGlyValArgGlu859095PheLeuGlnLysAlaHisGlnArgGlyIleSerMetAlaValAlaThr100105110SerAlaGlyLeuGluAsnThrArgPheIleLeuAspGlyLeuGlyIle115120125ThrAlaTyrPheSerAlaLeuValThrSerGluAspValHisArgGly130135140LysProAspProGluIlePheLeuIleAlaAlaGlnLysLeuGlyVal145150155160ProProGluAlaCysLeuValPheGluAspSerProAlaGlyLeuGlu165170175AlaAlaHisArgAlaGlyMetValSerValAlaLeuAlaThrThrPhe180185190ProAlaGluGlnLeuAlaGlyArgProGlyValLeuLeuValThrGly195200205AspTyrSerAlaIleAspLeuThrArgLeuIleValGlyThr210215220<210>9<211>259<212>PRT<213>Anaerolineathermophila<400>9MetThrThrSerLeuArgAspLeuLeuAspPheAlaValGluThrAla151015TyrLeuAlaGlyArgThrThrLeuAlaTyrPheGlnThrGlyValGln202530AlaGluPheLysAlaAspSerSerProValThrLeuAlaAspArgAla354045AlaGluGlnLeuIleArgGlnArgIleGluLysArgPheProHisHis505560AlaIleValGlyGluGluPheGlyValGlnGlySerAlaAspAlaThr65707580HisArgTrpPheIleAspProIleAspGlyThrLysSerPheLeuArg859095GlyIleProLeuTyrAlaValLeuLeuGlyLeuGluIleGluGlyArg100105110ValGlnValGlyValAlaTyrTyrProAlaMetAspGluMetLeuSer115120125AlaAlaAspGlyGluGlyCysTrpTrpAsnGlyArgArgAlaArgVal130135140SerThrAlaSerArgLeuAlaGluAlaTrpValThrSerThrAspPro145150155160TyrAsnPheGlnLysThrGlyLysAspAlaAlaTrpGlnArgIleGln165170175AlaValSerTyrHisArgGlyGlyTrpGlyAspAlaTyrGlyTyrLeu180185190LeuValAlaThrGlyArgAlaGluValMetLeuAspProIleMetAsn195200205GluTrpAspCysAlaProPheProProIlePheArgGluAlaGlyGly210215220PhePheGlyAspTrpGlnGlyAsnGluThrIleTyrGlyGlyGluAla225230235240LeuAlaThrThrGlnValLeuLeuProGluValLeuGluCysLeuHis245250255SerSerLeu<210>10<211>223<212>PRT<213>Anaerolineathermophila<400>10MetThrLysThrAsnIleLysGlyIleIlePheAspPheAspGlyLeu151015IleLeuAspThrGluThrProGluMetLeuAlaTrpGluLysAlaPhe202530ThrGluHisSerLeuSerPheProIleGluArgTyrLeuAsnSerIle354045GlyThrValSerAspAsnHisPheValGlnGlyPheMetGlnGluMet505560GlyLeuSerGluGlyGluIleGlnGlnThrValLeuAlaTyrGlnSer65707580HisIleAlaThrPheGluHisMetAsnHisProArgGluGlyValLeu859095AspLeuIleHisAspAlaGluLysIleGlyLeuArgLeuAlaValAla100105110SerAsnSerTyrArgThrTrpValValAspAsnLeuGlnArgLeuAla115120125LeuAspSerHisPheAspProIleCysThrArgAspAspValThrAsn130135140SerLysProAspProGluLeuTyrAsnLeuValLeuAlaLysTrpGly145150155160PheThrSerAsnGluValLeuAlaLeuGluAspSerProAsnGlyIle165170175LysAlaAlaLysAsnAlaGlyIlePheCysValAlaValProAsnPro180185190IleThrIleArgMetAspValThrLeuAlaAspLeuIlePheSerSer195200205PheSerAspPheSerLeuGlnGluIleLeuPheGlnLeuGluLys210215220<210>11<211>232<212>PRT<213>Anaerolineathermophila<400>11MetProLeuAspValSerArgIleGlnAlaIleCysPheAspIleAsp151015GlyThrLeuSerAspThrAspAspGluTrpAlaGluArgValLeuLys202530GlyIleSerPheLeuLysGlyTrpValGlyGluGlnArgLeuArgAsn354045LeuAlaArgTrpValValMetGluIleGluSerProGlyAsnLeuLeu505560TyrAsnLeuLeuAspArgLeuHisLeuAspAspGluAlaSerArgLeu65707580PheAsnPhePheValArgLysPheHisProArgProSerArgPheArg859095LeuIleSerGlyMetAspLysThrLeuGlnThrLeuAlaGlnTyrTyr100105110ProLeuAlaIleValSerAlaArgAspGluGluAlaThrLeuAlaPhe115120125LeuGlnGlnPheAspLeuThrSerLeuPheGlnValIleValSerAla130135140HisThrCysLysPheThrLysProPheProAspProIleLeuTrpAla145150155160AlaGluLysLeuAsnValProProSerAlaCysLeuMetValGlyAsp165170175ThrThrValAspIleHisAlaGlyLysLysAlaGlyAlaGlnThrVal180185190GlyValLeuCysGlyPheGlyThrHisLysGluLeuValCysAlaGly195200205AlaAspLeuIleLeuGluThrThrSerGluLeuAlaGluIleLeuLeu210215220LysGluLysAsnSerMetProAsn225230<210>12<211>252<212>PRT<213>Archaeoglobusfugidus<400>12MetAspGluArgAspAlaLeuArgIleSerArgGluIleAlaGlyGlu151015ValArgLysAlaIleAlaSerMetProLeuArgGluArgValLysAsp202530ValGlyMetGlyLysAspGlyThrProThrLysAlaAlaAspArgVal354045AlaGluAspAlaAlaLeuGluIleLeuArgLysGluArgValThrVal505560ValThrGluGluSerGlyValLeuGlyGluGlyAspValPheValAla65707580LeuAspProLeuAspGlyThrPheAsnAlaThrArgGlyIleProVal859095TyrSerValSerLeuCysPheSerTyrSerAspLysLeuLysAspAla100105110PhePheGlyTyrValTyrAsnLeuAlaThrGlyAspGluTyrTyrAla115120125AspSerSerGlyAlaTyrArgAsnGlyGluArgIleGluValSerAsp130135140AlaGluGluLeuTyrCysAsnAlaIleIleTyrTyrProAspArgLys145150155160PheProPheLysArgMetArgIlePheGlySerAlaAlaThrGluLeu165170175CysPhePheAlaAspGlySerPheAspCysPheLeuAspIleArgPro180185190GlyLysMetLeuArgIleTyrAspAlaAlaAlaGlyValPheIleAla195200205GluLysAlaGlyGlyLysValThrGluLeuAspGlyGluSerLeuGly210215220AsnLysLysPheAspMetGlnGluArgLeuAsnIleValAlaAlaAsn225230235240GluLysLeuHisProLysLeuLeuGluLeuIleLys245250<210>13<211>344<212>PRT<213>Archaeoglobusfugidus<400>13MetPheValMetPheIleValAlaGluValAspAspGluValCysVal151015LysArgGluValGluArgAlaAlaGlyGluValGlyValHisValSer202530LeuThrProPheGlnArgArgGluLysAlaGluLysAsnLeuTyrVal354045ValThrIleLeuGlyLysAspArgValGlyIleValArgAspIleThr505560ArgAlaPheLeuAspPheGlyIleAsnIleGluArgThrSerLeuThr65707580AlaArgGluGluLeuIleSerIleGluPheLeuValAspLeuGlyGln859095ArgAspAlaAlaGluValArgLysArgLeuArgArgGluAlaGluArg100105110LeuGlyLeuAspIleValMetGlnProTyrSerThrPheAsnArgGlu115120125LysArgLeuIleValPheAspMetAspSerThrLeuValGluAlaGlu130135140IleIleAspGluLeuAlaLysGluAlaGlyValGlyAspGluValSer145150155160LysLeuThrGluArgAlaMetArgGlyGluIleGlyPheLysGluAla165170175LeuGluGluArgValArgLeuLeuLysGlyLeuProValGluValLeu180185190GluArgIleTyrSerArgIleLysLeuThrGluGlyAlaLysGluLeu195200205ValArgSerLeuLysGluAlaGlyTyrLysValAlaValValSerGly210215220GlyPheSerTyrPheThrAspArgLeuLysGluGluLeuGlyLeuAsp225230235240TyrAlaPheGlyAsnGluLeuGluIleGluAsnGlyArgLeuThrGly245250255ArgIleLysGlyArgIleIleAspAlaSerGluLysAlaArgIleVal260265270GluGluIleAlaArgLysGluGlyIleSerProGluAsnValValAla275280285ValGlyAspGlyAlaAsnAspArgLeuMetIleGluArgAlaGlyLeu290295300GlyIleAlaPheAsnAlaLysGluValLeuLysAspValAlaAspGly305310315320SerIleSerLysGluAsnLeuValGlyLeuAlaSerValLeuLysLeu325330335ProAlaGluPheArgLysLysVal340<210>14<211>277<212>PRT<213>Archaeoglobusfugidus<400>14MetLeuLysThrProSerAspAlaAlaValAlaGlyCysLeuSerLeu151015LeuLeuGluValSerGlyAsnProLysAlaGlyAsnValAspArgGlu202530HisAspPheAspAspLeuLysPheGluHisPheLeuAlaSerAlaAla354045GlyAlaPheProAlaPheLeuGluValAlaGluLysArgIleIleGly505560GluGlyValLeuArgAlaValLysGluSerMetArgTrpHisArgAla65707580GluAsnValHisPheGlyAlaPheLeuLeuLeuValProLeuIleSer859095SerTrpAspAlaGlyGlyMetValAspIleAlaGluAlaAlaArgAsn100105110ArgLeuArgArgThrAspPheArgAspSerLeuSerValLeuGluAla115120125PheArgLeuSerAsnAlaArgValValGluAlaGlyGluLeuAsnLeu130135140LysAspArgLysThrGluGluGluIleAlaGlnLysLysIleAsnLeu145150155160TyrGluTrpMetLysMetAlaProGluGluAsnLeuIleAlaArgGlu165170175LeuValAspGlyPheLysIleSerIleGluGlyAlaLysPheLeuLeu180185190SerPheGlyAsnSerGlyLysAlaValValGluLeuTyrTyrHisLeu195200205LeuSerLysPheProAspProLeuValIleAlaLysMetGlyArgGlu210215220TyrAlaGluLysIleThrGluTrpAlaGluLysAlaArgThrGluGlu225230235240GluArgLysGluLeuAspGluLysLeuLeuLysAspGlyAlaAsnPro245250255GlyThrIleAlaAspLeuThrAlaSerSerIlePheLeuAlaLeuAla260265270GluGlyTrpArgIle275<210>15<211>219<212>PRT<213>Archaeoglobusprofundus<400>15MetPheLysAlaLeuValValAspIleAspGlyThrLeuThrAspLys151015LysArgAlaIleAsnCysArgAlaValGluAlaLeuArgLysLeuLys202530IleProValValLeuAlaThrGlyAsnIleSerCysPheAlaArgAla354045ValAlaLysIleIleGlyValSerAspIleValIleAlaGluAsnGly505560GlyValValArgPheSerTyrAspGlyGluAspIleValLeuGlyAsp65707580ArgSerLysCysLeuArgAlaLeuGluThrLeuArgLysArgPheLys859095ValGluLeuLeuAspAsnGluTyrArgLysSerGluValCysMetArg100105110ArgAsnPheProIleGluGluAlaArgLysIleLeuProLysAspVal115120125ArgIleValAspThrGlyPheAlaTyrHisIleIleAspAlaAsnVal130135140SerLysGlyLysAlaLeuMetPheIleAlaAspLysLeuGlyLeuAsp145150155160ValLysAspPheIleAlaIleGlyAspSerGluAsnAspIleGluMet165170175LeuGluValAlaGlyPheGlyValAlaValAlaAsnAlaAspGluLys180185190LeuLysGluValAlaAspLeuValThrSerLysProAsnGlyAspGly195200205ValValGluAlaLeuGluPheLeuGlyLeuIle210215<210>16<211>224<212>PRT<213>嗜热硫酸盐还原菌(Archaeoglobusveneficus)<400>16MetLeuArgProLysGlyLeuAlaIleAspIleAspGlyThrIleThr151015TyrArgAsnArgSerLeuAsnCysLysAlaValGluAlaLeuArgLys202530ValLysIleProValValLeuAlaThrGlyAsnIleSerCysPheAla354045ArgThrAlaAlaLysLeuIleGlyValSerAspIleValIleCysGlu505560AsnGlyGlyIleValArgPheSerTyrAspGlyAspAspIleValLeu65707580GlyAspIleSerLysCysLeuLysAlaAlaGluIleLeuLysGluTyr859095PheGluIleGluPheLeuAspAlaGluTyrArgLysSerGluValCys100105110LeuArgArgAsnPheProIleGluGluAlaArgLysIleLeuHisAsp115120125AlaLysLeuAspValLysIleValAspSerGlyPheAlaTyrHisIle130135140MetAspAlaLysValSerLysGlyArgAlaLeuGluTyrIleAlaAsp145150155160GluLeuGlyIleSerProLysGluPheAlaAlaIleGlyAspSerGlu165170175AsnAspIleAspLeuIleLysAlaAlaGlyLeuGlyIleAlaValGly180185190AspAlaAspLeuLysLeuLysMetGluAlaAspValValValSerLys195200205LysAsnGlyAspGlyValValGluAlaLeuGluLeuLeuGlyLeuIle210215220<210>17<211>194<212>PRT<213>地衣芽孢杆菌(Bacilluslicheniformis)<400>17MetLeuHisLeuGlyIleAspIleAspGlyThrIleThrAlaGlnAsp151015ThrPheValProTyrLeuAsnGluSerPheSerLeuSerIleThrLeu202530GluAspIleLysGluTyrAspLeuThrLysLeuLeuAsnIleSerGln354045GluAspPheTrpLysTrpMetAsnGluAsnGluProArgIleTyrGln505560GluAlaLeuLeuAlaGlnTyrAlaLysGlnThrLeuAspGluLeuLys65707580LysGlnHisLysLeuIleTyrIleThrAlaArgArgHisHisLeuGlu859095AspIleThrTyrAspTrpPheGluLysArgAspIleHisTyrAspHis100105110IleGluLeuValGlyGlyHisAspLysLeuGluAlaValPheArgHis115120125GluIleAspValPhePheGluAspHisHisGlyAsnAlaThrMetIle130135140AlaLysGluAlaGlyIleProValLeuLeuPheAsnThrProTyrAsn145150155160GlnLeuProThrAspSerAsnIleValArgValAspSerTrpLeuGlu165170175AlaAlaGluTrpLeuLysLysAsnGluLysThrLeuLysAlaAlaLys180185190SerMet<210>18<211>226<212>PRT<213>地衣芽孢杆菌(Bacilluslicheniformis)<400>18MetLysAlaValIlePheAspLeuAspGlyValIleThrAspThrAla151015GluTyrHisTyrLeuAlaTrpLysHisThrAlaGluGlnIleGlyIle202530GluIleAspArgSerPheAsnGluArgLeuLysGlyIleAsnArgGlu354045GlnSerLeuAspLysIleLeuIleHisGlyGlyAlaAlaGlyLysPhe505560GlnGluAlaGluLysGlnGluIleMetArgArgLysAsnGlnTyrTyr65707580GlnGlnLeuIleGlnAsnLeuThrProHisAspLeuLeuProGlyIle859095SerValLeuPheAlaGluLeuLysArgGluHisIleSerIleAlaLeu100105110AlaSerSerSerArgAsnAlaProAlaIleLeuGlnArgLeuGlyVal115120125MetAspGluPheGlnGlyValValAspProAlaAlaLeuAlaHisGly130135140LysProAspProGluIlePheLeuThrAlaAlaAlaLeuLeuGlyVal145150155160ProProSerGluCysAlaAlaIleGluAspAlaGluAlaGlyIleAla165170175AlaIleLysSerAlaGlyMetPheAlaValGlyValGlyAspGluThr180185190SerLeuArgGlyAlaAspLeuIleValHisAsnThrAsnGluLeuThr195200205PheGluLeuLeuAsnGluGlyTrpGlnArgTyrCysCysIleArgGlu210215220GlyLys225<210>19<211>216<212>PRT<213>地衣芽孢杆菌(Bacilluslicheniformis)<400>19MetLysLysTrpAlaPheValSerAspPheAspGlyThrIleSerLys151015GlnAspPheTyrTrpMetValIleAspLysTyrPheProGluGlyArg202530GluLeuPheLysLysTrpLysSerGlyGluLeuLysAspIleGluPhe354045LeuGlyThrValPheAlaSerIleAsnGlnSerGluGlnLysIleIle505560AspAspIleHisSerIleProIleAspGluTyrValProAspPheIle65707580GlnHisValGlnLysSerGlyGlyAspPheTyrIleLeuSerAlaGly859095ThrAspTyrTyrIleHisTyrIleLeuLysLysTyrGlyIleThrAsp100105110ValGluValTyrSerAsnLysGlyPhePheLysGluAspAsnValHis115120125MetAspIleAspGluAsnHisTrpHisTyrSerGluArgTyrGlyIle130135140AspLysSerLysValIleGlnLysLeuLysGluGluTyrGluThrVal145150155160TyrPheAlaGlyAspSerGluProAspSerHisProAlaLysPheAla165170175AspValThrPheAlaLysAspAlaLeuGlnAspLeuLeuArgGlnGln180185190GlyValProPheValAlaValGluThrPheGluAspIleGluGlnTyr195200205LeuLysGluLysGlyArgIleVal210215<210>20<211>223<212>PRT<213>热解纤维素菌(Caldicellulosiruptorbescii)<400>20MetGlyLysIleLysAlaAlaIlePheAspMetAspGlyValLeuThr151015AspThrValLysLeuHisPheLysAlaTrpLysLysMetPheGluSer202530HisGlyTyrLysPheGluTyrGluAspTyrLysTrpLysValAspGly354045LysProArgIleAspGlyIleArgSerIleAlaTyrAspMetProGlu505560AspLysLeuIleGluMetAlaGluGluLysGlnLysIlePheLeuGlu65707580PheValGluGlnGluAsnLeuGluAlaPheGluAspSerIleTrpLeu859095LeuAsnHisLeuLysGlnAsnAspIleLysLeuAlaValAlaSerSer100105110SerLysAsnThrThrLysIleLeuThrLysIleGlyIleTyrAsnMet115120125PheAspThrIleValThrGlyTyrAspPheLysLysGlyLysProAsp130135140ProGluIlePheLeuThrAlaAlaGlnArgLeuAsnValAsnProLys145150155160GluCysValValPheGluAspAlaIleAspGlyValLysAlaGlyIle165170175ArgAlaGlyMetLeuThrIleGlyValCysArgAspGlyGlnPheAsp180185190ArgLeuLysGluAlaHisTyrValValAspArgLeuAspLysIleSer195200205LeuGluLeuLeuGluAsnLeuHisGluLysLeuPheLysLysVal210215220<210>21<211>275<212>PRT<213>热解纤维素菌(Caldicellulosiruptorbescii)<400>21MetLysAsnSerSerIleLeuLysAsnIleAspLeuPheLeuLeuAsp151015LeuAspGlyThrValTyrLeuGlyGluLysValPheGluGlyAlaArg202530GluPheIleLysLeuLeuAsnLysAsnGlnLysGluPheLeuPheLeu354045ThrAsnAsnSerSerLysSerSerGluGluTyrTyrSerLysLeuLeu505560AsnMetGlyPheGluIleThrLysGluAsnValPheThrSerGlyGln65707580AlaMetGlyIleTyrIleLysThrIleHisLysLysGluLysProPro859095ArgValTyrValValGlyThrThrSerLeuLysArgGluLeuLysSer100105110MetGlyIleValValValAspSerProAsnTyrAsnIleAspTyrLeu115120125ValIleGlyPheAspThrThrLeuThrTyrLysLysLeuLeuAspAla130135140CysGluLeuIleArgArgGlyValProPheLeuAlaThrAsnProAsp145150155160LeuValCysProLeuAspGlyGlyArgTyrIleProAspCysGlySer165170175IleCysIleMetLeuGluAsnAlaThrLysLysLysProValPheVal180185190GlyLysProSerSerIleMetValAspIleIleSerAsnLeuLysLys195200205ValGluLysSerArgIleAlaMetIleGlyAspArgLeuTyrThrAsp210215220MetLysMetAlaLysAspSerGlyMetValAlaAlaLeuValLeuSer225230235240GlyGluThrLysMetLysAspValGluAlaSerThrLeuLysProAsp245250255LeuIleTyrGlySerIleLysAspMetTyrGluGluLeuLysLeuVal260265270PheGlyGly275<210>22<211>266<212>PRT<213>热解纤维素菌(Caldicellulosiruptorbescii)<400>22MetIleLysLeuValAlaThrAspLeuAspAspThrLeuLeuSerLys151015AspLeuThrIleThrGluLysAsnLeuAsnAlaIleGluPheLeuLys202530LysAsnAsnIleIlePheIleLeuAlaSerGlyArgProTyrProSer354045IleLysAsnValAlaTyrAspLeuGlnAsnPheTyrProMetIleThr505560TyrGlnGlyAlaLeuValTyrAspProLysAsnAspLysLysLeuTyr65707580GlyCysGluIleLysProGluAspAlaLysGluLeuValArgLeuAla859095LysAspGluGlyIleHisValHisIleTyrIleAspAsnValTrpTyr100105110ValGluAlaMetAsnGluLysThrGluTyrTyrArgAsnLeuThrLys115120125LeuGluProHisIleValLysAsnLeuLeuGluPheIleAspArgPro130135140ValThrLysValLeuPhePheAspGluHisGluArgLeuLysAspLeu145150155160LysGluSerLeuProAspAspPheSerLysLysPheAsnIleMetPhe165170175SerLysProPhePheLeuGluPheThrAspIleAsnValSerLysGly180185190AsnAlaLeuLysPheLeuThrGluTyrTyrGlyLeuLysArgGluGlu195200205ValMetAlaIleGlyAspGlyAspAsnAspIleSerMetIleGluTyr210215220AlaGlyIleGlyValAlaValGluAsnAlaValGluLysLeuLysGlu225230235240AlaAlaAspPheValValAlaLysSerAspAspSerGlyPheAlaGln245250255AlaIleGluLysValPheAsnValHisPhe260265<210>23<211>279<212>PRT<213>热解纤维素菌(Caldicellulosiruptorbescii)<400>23MetTyrLysLeuIleAlaIleAspLeuAspMetThrLeuLeuAspLys151015AsnLysAsnIleSerSerArgAsnLysArgAlaIleGluLeuValLys202530GlnLysGlyValGlnIleValLeuCysSerGlyArgIleLeuLysGly354045ValMetTyrPheAlaLysValLeuGlyLeuTyrAspGlnValIleVal505560AlaCysAsnGlyAlaIleValArgAspLeuLysLysAsnAsnAspIle65707580TyrTyrIleGlyLeuGluAsnSerLysSerLeuGluIleAlaArgIle859095CysLysGluAsnAspIleTyrTyrHisTyrTyrPheGlnAspThrMet100105110IleAlaArgArgLeuAspTyrSerSerLysPheTyrTyrGluLysAsn115120125LysGluLeuProGluGluGluArgIleAsnIleIleIleAspAspSer130135140GluAsnThrIleAsnAlaCysGlyAspLeuIleThrLysPheValIle145150155160IleAspLysAspLeuGluLysValAsnTyrValArgLysIleIleGlu165170175ArgGluIleProGlyValGluThrThrLysSerAspIleAsnIleLeu180185190GluValMetLysGluGlyValAsnLysLysArgAlaLeuGluPheVal195200205IleSerTyrLeuGlyIleAlaProGluGluValMetAlaIleGlyAsp210215220AsnGluAsnAspLeuGluMetIleGluPheAlaGlyLeuGlyValAla225230235240MetGlyAsnAlaIleGluGluLeuLysLysIleAlaAspTyrValThr245250255SerSerTyrGluAsnAspGlyValAlaArgAlaIleGluLysPheVal260265270LeuGlyGluAlaValAsnVal275<210>24<211>284<212>PRT<213>热解纤维素菌(Caldicellulosiruptorbescii)<400>24MetIleLysLeuIleAlaLeuAspIleAspGlyThrLeuLeuAspAsp151015ArgGlyTyrIleProGlnIleAsnArgGluPheLeuLysIleAlaVal202530GluLysTyrLysIleValIleValLeuCysThrGlyArgGlyAlaSer354045AlaPheLysIleAlaLysAspLeuGlnLeuProCysSerLeuIleSer505560AlaAsnGlyValTyrValPheGluAsnProAsnPheProProIleIle65707580LysAsnTyrLeuThrGluArgGlnLysLysValLeuIleGluPheLeu859095AspAsnAsnHisPheGluIleAspTyrTyrIleValLeuGlyTyrGlu100105110GlnAspPheHisMetIleTyrLysGluArgThrAsnTyrAspSerTyr115120125PheLeuSerPheValAsnGlyArgLysGlnPheLysSerThrTyrPro130135140AlaGluLysLeuLeuLysPheLeuGluTyrProIleSerHisThrGly145150155160IleValGlyLysTyrGluLysLeuLysGluIleLysGluIleLeuLys165170175ThrLeuGluLeuAspCysAsnIleIleLeuTyrTyrAlaSerAspAsn180185190LysGluTyrGlyPheLeuGluValLeuSerAsnAsnAlaSerLysGlu195200205LysAlaLeuLeuGlnPheMetAsnPheLysAsnIleSerSerGluGlu210215220LeuIleSerIleGlyAspAsnPheAsnAspValGlyMetPheLysIle225230235240SerGlyIleSerValAlaValAlaAsnAlaProGluGluValLysLys245250255AlaAlaLysPheValThrSerArgThrAsnAsnGluGlyAlaValAla260265270GluAlaIleGluMetPheIleIleLysArgAspGly275280<210>25<211>286<212>PRT<213>Caldilineaaerophila<400>25MetArgProPheAspLeuValValLeuAspLeuAspGlyThrIleLeu151015AsnAlaTyrGlnArgThrArgIleSerLysAlaValHisAspAlaIle202530GluAlaValGlnAlaAlaGlyValProValThrIleGlyThrGlyArg354045ThrLeuAspTyrIleArgTyrHisLeuProGlyAspLeuHisLeuThr505560TyrProValIleAlaThrGlnGlyAlaValIleGlyAspProValSer65707580GlyArgValLeuValGluIleProLeuProLeuLysGluAlaArgAla859095IleAlaAlaPheValAspAspHisArgTyrThrThrAlaPheTyrPhe100105110AsnAsnAlaAspGlyHisThrValIleTyrGlnAsnAlaAlaGlyAla115120125ThrAlaGluGluGluAlaLeuLeuHisHisLeuLeuGlyValProAsn130135140AlaLeuValGluGlnPheSerProLeuLeuAlaAsnGluAspAlaHis145150155160ProProIleLysValIleSerPheAsnHisGlyLeuProAspAlaVal165170175AspLeuLeuHisGluTyrGlnArgArgPheSerProProLeuThrVal180185190ThrArgThrHisGluTrpLeuValGluAlaThrAlaProGlyValAsp195200205LysGlySerGlyLeuLeuArgLeuCysLysLeuLeuGlyValAspPro210215220GlnArgValLeuAlaIleGlyAspSerAspAsnAspIleProMetLeu225230235240GluValAlaGlyPheAlaIleAlaMetGlyAsnAlaAsnGluArgVal245250255LysAlaValAlaAspTrpValAlaProSerIleAspGluGluGlyAla260265270AlaValAlaLeuArgArgTrpValLeuGluProMetSerAla275280285<210>26<211>256<212>PRT<213>Caldilineaaerophila<400>26MetLysAspLysThrLysValLeuAlaLeuAspLeuAspAspThrLeu151015LeuArgSerAspLysSerIleGlyAlaAlaThrLeuAlaAlaLeuAsp202530ArgTrpLeuAlaAlaGlyHisGluIleValValAlaThrGlyArgPro354045ProArgAsnValAlaProValLeuProProSerLeuLeuHisValPro505560ArgIleValTyrAsnGlyAlaGlnAlaIleLeuGlyAspGluIleIle65707580PheAspArgProIleProAlaGlnAspValArgHisIleLeuGluTrp859095SerLysArgSerGlyGluAlaTrpTyrIleGlyLeuGluIleAsnAsp100105110MetLeuTyrValAsnArgHisPheGluLysProGlyThrPheGlnVal115120125ValAspLeuMetGlnLeuCysAspGlnProValTyrLysIleIlePhe130135140PhePheProAspGlyArgArgAsnIleGluProLeuLeuAlaAlaMet145150155160ProProThrThrArgAlaLeuValThrProLysPheSerLeuValGln165170175LeuCysAlaArgAspAlaAsnLysPheThrAlaLeuLysGlnLeuLeu180185190AlaArgArgProLeuAlaProAlaAlaValIleAlaValGlyAspAsp195200205IleAsnAspIleGluMetValArgCysSerAspIleGlyValAlaVal210215220AlaAsnAlaLeuProGluValLysAlaValAlaAspTrpIleAlaPro225230235240GlyAlaAspAspAspGlyValAlaTyrValIleAspArgLeuLeuAsn245250255<210>27<211>223<212>PRT<213>Caldilineaaerophila<400>27MetThrIleArgAlaIleLeuTrpAspLeuAspGlyThrLeuAlaAsp151015ThrThrAlaLeuHisTyrGlnAlaTrpArgLysThrMetGlnArgTyr202530GlyValAspLeuThrTyrGluSerPheIleLysAspTyrGlyArgAsn354045AsnAlaGluIleLeuAlaGluHisPheGlyIleHisAlaValAlaThr505560IleGlnGlnValAlaAspGluLysGluSerAlaPheArgSerLeuIle65707580ThrProGlyValLeuGlnProLeuProGlyAlaLeuAlaTrpLeuHis859095CysPheArgSerLeuGlyMetIleGlnValIleGlySerSerGlyPro100105110MetAlaAsnIleAlaAlaValValHisAlaLeuGlyValGlyAspPhe115120125PhePheGlyLeuValSerGlyValHisLeuProGluGlyLysProGlu130135140ProThrIlePheLeuArgCysAlaAlaLeuAlaGlyAlaAlaProAsp145150155160GluCysLeuValIleGluAspSerLeuHisGlyIleGluAlaAlaMet165170175ArgAlaGlyMetThrSerValAlaValGlyArgIleAlaAlaGlnPro180185190AlaLeuGlnAspLeuIleGlnLysAsnProGlyLeuArgCysIleVal195200205ThrProSerLeuAlaSerIleAspAspProLysSerIleLeuThr210215220<210>28<211>275<212>PRT<213>Caldithrixabyssi<400>28MetSerGluValMetLysLysMetLeuValLeuAspPheAspGlyThr151015LeuPheGlnProGlyLysPheIleSerAspGlnAspLeuLysLeuLeu202530LysAlaLeuAlaAsnMetAsnValValArgValIleAlaThrGlyArg354045SerLeuPheSerLeuLysLysValIleAspMetSerPheProIleAsp505560TyrLeuIleLeuSerThrGlyValGlyIleLeuAspTrpArgSerGln65707580LysLeuIleArgSerTyrAspMetGluGlyGlyLeuValAspGluIle859095IleAlaIleLeuLysArgArgArgHisSerPhePheValHisGlnPro100105110AlaProGlnAsnHisLysPhePheTyrTyrAspGlyIleAlaProArg115120125GlnAspPheThrArgArgPheGluLeuTyrArgAspPheAlaArgPro130135140LeuLysGlnArgLysArgGlyMetAlaAlaGlyGlnIleLeuValIle145150155160SerGluGluLeuGlnGlnIleLysGluGluLeuGlnProLeuMetHis165170175ArgIleAsnIleIleArgThrThrSerProLeuAspGlyArgSerMet180185190TrpValGluIlePheAspLysArgValAsnLysAlaLeuAlaAlaGln195200205PheLeuAlaGluArgLeuAsnIleSerValArgAsnIleValAlaLeu210215220GlyAsnAspTyrAsnAspLeuAspLeuLeuAsnTrpAlaGlySerAla225230235240PheValValHisThrAlaProGluGluLeuLysGluLysPheGlnIle245250255IleLysAsnAlaAspAspGlyLeuLeuIleHisIleMetProLeuLeu260265270GlnArgGly275<210>29<211>171<212>PRT<213>Caldithrixabyssi<400>29MetArgLysLysLeuSerGlnIleGlnLeuIleLeuMetAspValAsp151015GlyValLeuThrAlaGlyGluIleIleTyrSerAlaAlaGlyAspGlu202530LeuLysMetPheAsnValGlnAspGlyMetGlyIleThrLeuAlaArg354045MetAlaGlyLeuLysThrGlyIleLeuThrGlyArgLysSerGluLeu505560ValArgArgArgAlaGluGluLeuLysIleAspIleValSerGlnGly65707580SerPheGluLysLeuProGluTyrGluLysIleLysGlnGlnMetGln859095LeuSerAspGluGlnIleCysTyrIleGlyAspAspValLeuAspIle100105110ProValLeuLysArgValGlyPheSerValAlaValAlaAsnAlaArg115120125AspGluValLysAlaIleAlaAspTyrValThrValAlaGluGlyGly130135140LysGlyAlaValArgGluValIleAspLysIleLeuLysTrpGlnAsn145150155160LysLeuHisAlaLeuIleAspGlnLeuSerArg165170<210>30<211>354<212>PRT<213>Caldithrixabyssi<400>30MetLysArgGlyHisArgTyrIleValPheThrMetAlaIlePheSer151015LeuIleLeuIleAlaCysThrLysLysGluValLysProAspLeuAla202530PheAlaGlyGluLysHisLeuLysAsnIleArgMetLeuThrAsnGly354045GlyGluAsnAlaGluAlaTyrPheSerPheAspGluSerGlnLeuIle505560TyrGlnSerLysHisAspSerIleLysCysAspGlnIlePheIleMet65707580AsnLeuAspGlySerAspLysArgMetValSerThrGlyLysGlyArg859095ThrThrCysSerTyrPheLeuProGlyAspGlnGlnIleIleTyrAla100105110SerThrHisGlyValAspGluAsnCysProProProProAspPheSer115120125ArgGlyTyrValTrpLysValTyrSerSerTyrAspLeuTyrIleAla130135140AsnAlaAspGlySerAspProGlnProPheLeuProSerProGlyTyr145150155160AspAlaGluAlaThrValSerProArgGlyAspLysIleValPheThr165170175SerGlnArgAsnGlyAspLeuAspIleTyrThrValAsnIleAspGly180185190SerGlyLeuLysGlnLeuThrHisGluIleGlyTyrAspGlyGlyAla195200205PhePheSerTrpAspGlySerLysIleValTyrArgAlaTyrHisPro210215220ArgThrGluGluGluLeuLysArgTyrArgLysLeuLeuAlaGluGlu225230235240LeuIleGluProAsnAsnPheGlnLeuPheValMetAspAlaAspGly245250255SerAsnLysArgGlnIleThrHisAsnAspPheAlaAsnPheAlaPro260265270PhePheHisProAspAsnLysArgIleIlePheCysSerAsnMetGly275280285ThrThrGluSerThrGlnArgAspPheAsnLeuTrpMetIleAsnGlu290295300AspGlyThrGlyLeuLysGlnIleThrPhePheLysGlyPheAspGly305310315320PheProMetPheThrHisAspGlyLysLysLeuValPheAlaSerAsn325330335ArgPheAsnLysLysLysGlyGluThrAsnValPheIleAlaAspTrp340345350ValGlu<210>31<211>222<212>PRT<213>Caldithrixabyssi<400>31MetArgAsnLysAlaValLeuPheAspPheAspGlyValValValLys151015SerMetGluGlnHisPheAsnAlaTrpArgGlnAlaPheLeuGluLys202530GlyValGluIleLysGluAspGluPhePheValLeuGluGlyGlnGly354045IleAsnThrIleAlaHisHisLeuGlyLysIleTyrGlyLeuAsnArg505560GlnGlnValGluGluValMetGluArgLysValAsnTyrTyrAsnGln65707580PheMetThrLeuGluPheTyrAspHisPheHisGluLeuValGluHis859095LeuHisArgArgGlnValProMetGlyValValThrGlyGlyAsnArg100105110SerArgValGluLysIleIleAsnGluHisPheAsnHisTyrPheArg115120125AlaLeuValThrValAspAspValGluArgGlyLysProPheProAsp130135140ProPheLeuLysAlaAlaGlnMetLeuAsnMetAlaProGlnAsnCys145150155160IleValValGluAsnAlaProMetGlyIleLysGlyAlaLysArgAla165170175GlyMetThrValValAlaIleThrThrThrLeuLysProAspTyrLeu180185190LysGlnAlaAspTyrIleAlaHisAsnPheLeuGluValGluGluIle195200205LeuAsnThrLeuLeuGlyIleGluArgThrGluThrIleLys210215220<210>32<211>268<212>PRT<213>Carboxydocellasp.ULO1<400>32MetValLeuValLysLeuIleAlaIleAspLeuAspAspThrLeuLeu151015ArgThrAspCysThrIleSerProArgAlaGlnAlaAlaIleLysAla202530AlaSerAlaArgGlyValAlaValThrLeuAlaThrGlyArgMetTyr354045ArgSerAlaArgProTyrAlaLeuGluLeuGlyLeuAspLeuProLeu505560IleThrTyrGlnGlyAlaLeuIleLysSerGlyLeuSerGlyGluGlu65707580LeuTrpHisCysProLeuProProGluMetAlaAlaGluIleIleThr859095LeuAlaArgGluGluGlyIleHisAlaAsnIleTyrLeuAspAspHis100105110LeuTyrValGluLysAlaThrAlaGluAlaGluAlaTyrSerGlnLeu115120125AlaArgValProTyrThrValGluGluAspLeuArgGlyArgIleLeu130135140AlaSerGlyGlnGlyProSerLysIleLeuLeuIleAlaGluProGlu145150155160ArgLeuAspGlnLeuAlaAlaAlaLeuArgProArgTrpGlnGlyLys165170175ValGlnLeuAlaLysSerLysAspHisTyrLeuGluPheThrHisPro180185190GluAlaSerLysGlyGlnAlaLeuIleAlaLeuCysArgTyrLeuAsp195200205LeuGluLeuLysAspAlaMetAlaIleGlyAspSerPheAsnAspLeu210215220AspMetIleGluLeuAlaGlyIleGlyValAlaMetGlyAsnAlaLys225230235240ProGluValLysAlaIleAlaAspTyrValThrLeuAlaAsnAspAsp245250255AspGlyValAlaGluAlaIleHisArgPheValLeu260265<210>33<211>266<212>PRT<213>Carboxydothermusferrireducens<400>33MetArgTyrArgLeuLeuAlaValAspLeuAspAspThrPheLeuAsn151015LysGluLeuGlnValSerProArgValGlnGlnAlaValIleGluAla202530LeuLysLysGlyIleIleValThrLeuAlaThrGlyArgMetTyrArg354045SerAlaLysLysTyrAlaPheSerPheLeuGlyAspIleProLeuIle505560ThrTyrAsnGlyAlaLeuIleLysTyrSerArgSerGluArgGluIle65707580TyrHisGlnProValProGlyGluLeuAlaLeuThrIleTyrArgArg859095ValLysGlyHisPheHisLeuAsnValTyrGlnAspAspGluLeuPhe100105110ValGluGluAspAsnGlnTyrIleArgAspTyrSerLysIleAlaGly115120125ValProPheArgValValAsnAsnIleGluGluLeuLeuGlyLysLys130135140AlaProThrLysLeuLeuAlaIleGlyAspProGluGluLeuAspGln145150155160LeuTrpGluGluThrAsnGlyGluPheArgGlyValLeuHisIleThr165170175LysSerLysProHisTyrLeuGluPheLeuAlaAlaGlyValAsnLys180185190GlyGluAlaLeuLysIleLeuAlaGlnHisLeuArgIleSerLeuLys195200205GluThrValAlaValGlyAspSerTyrAsnAspLeuGluMetLeuGlu210215220AlaAlaGlyLeuGlyValAlaMetGlyAsnAlaLeuProGluValLys225230235240ArgArgAlaAspLeuValValProAlaAsnAspGluAspGlyIleAla245250255TyrLeuIleAsnGluIleIleLeuLysAsn260265<210>34<211>231<212>PRT<213>Carboxydothermusferrireducens<400>34MetAlaTyrIleLeuPheAspLeuAspGlyThrLeuLeuProLeuAsn151015LeuAspTyrPheLeuLysLeuTyrIleGlnGluValLysSerTyrVal202530LysGlyLysPheAspProAspLeuIleValLysAlaIleLeuLysAla354045ThrGluGluMetValLysAsnThrGlyGluLysLeuAsnSerGluVal505560PheTrpGlnAlaPheAspArgValTyrProGlyThrAlaAsnLeuHis65707580AsnValPheGluAspPheTyrHisThrSerPheLysAsnIleGlyAsn859095TyrPheLysProHisProLeuAlaArgProMetIleArgTyrLeuHis100105110LysLysGlyHisThrLeuIleLeuAlaThrAsnAlaLeuPheProArg115120125SerAlaIleIleGluArgLeuTyrTrpAlaAsnLeuSerProLysTyr130135140PheArgPheIleThrHisTyrAspAsnMetHisTyrCysLysProAsn145150155160ProAsnTyrTyrLysGluIleLeuGluLysIleLysAlaArgProSer165170175AspCysLeuMetIleGlyAsnAspProAlaGluAspLeuValAlaLys180185190GluLeuGlyIleLysThrPheLeuValLysAspTyrGluValLysArg195200205GluAsnProArgThrProAspTyrAlaGlyAspPheTyrGluLeuTyr210215220ArgPheIleArgGluAsnPhe225230<210>35<211>212<212>PRT<213>Carboxydothermusferrireducens<400>35MetIleLysAlaValPhePheAspLeuAspGlyThrLeuLeuAspThr151015PheAspLeuIleTyrGluSerPheLysHisValTyrLysAsnPheLeu202530AsnLysAspIleThrArgGluGluIleTyrProTyrPheGlyLysPro354045LeuIleTyrSerPheGluAsnLeuAspProGluThrIleAspGlnVal505560IleAlaAlaTyrArgGluPheAsnLeuGlnHisHisAspGlnMetVal65707580LysProPheProGlyAlaLysGluThrLeuGluLysLeuLysGlnArg859095GlyLysIleLeuAlaValIleThrSerLysValLysSerThrAlaIle100105110ArgGlyLeuLysLeuPheAsnLeuAspArgTyrPheAspLeuValVal115120125AlaLeuGluAspThrGluLysHisLysProAspProAlaProValLeu130135140TyrAlaLeuLysPhePheGlnValLysProGluGlnCysLeuMetVal145150155160GlyAspSerProHisAspMetValSerAlaGlnArgAlaGlyValLys165170175ThrAlaAlaValLysTrpSerValLeuProTrpGluAspLeuValLys180185190ThrLysProAsnTyrIleLeuAsnSerPheAspAspLeuLeuLysIle195200205ThrGlyValGlu210<210>36<211>218<212>PRT<213>绿弯菌门细菌54-19(Chloroflexibacterium54-19)<400>36MetAsnLysPheGlnGlyValIleLeuAspIleAspGlyThrLeuIle151015AspSerAsnAspAlaHisAlaHisSerTrpValAspThrPheAlaGlu202530AlaGlyIleProValProPheGluArgValArgProLeuIleGlyGlu354045GlyGlyAspLysLeuLeuProGluValSerGlyPheGluAlaGlySer505560AlaGluGlyLysArgLeuThrLysArgArgThrGluIlePheLysGlu65707580LysTyrLeuProHisIleGlnProPheProLysThrArgGluLeuIle859095ThrAlaPheGluLysArgGlyLeuLysLeuGlnIleAlaThrSerAla100105110GlnGluAlaGluValLysAspLeuLeuLysValAlaGlyThrProGlu115120125IleIleGluGlnLysThrThrSerAspAspAlaGluLysSerLysPro130135140AspProAspIleIleHisAlaAlaLeuGlnLysIleGlyLeuGluAla145150155160GlyGlnValValMetLeuGlyAspThrProTyrAspValAlaAlaAla165170175LysLysAlaGlyValLysSerTyrAlaPheArgCysGlyGlyTrpTrp180185190GlnAspArgAspPheGluAspAlaAlaAlaIlePheAspGlyProAla195200205AspLeuLeuGluLysLeuAspAspTyrLeu210215<210>37<211>284<212>PRT<213>突尼斯废水袍菌(Defluviitogatunisiensis)<400>37MetAsnTyrHisLeuAsnSerSerThrLysValPheValPheAspPhe151015AspGlyThrLeuLeuAsnSerGluValArgIleSerProArgThrPhe202530GluAlaLeuLysLysLeuLysGluHisGlyHisThrIleIleLeuCys354045SerGlyArgMetTyrAlaSerMetLeuPheIleValGluAsnPheLeu505560ProPheLeuLysGlyTyrAlaHisIleValSerTyrAsnGlyGlyTyr65707580IleValAsnAsnLysGlyGluLeuLeuPheGluGluGlyLeuAspLys859095AspValAlaIleAspCysIleGluPheLeuArgGluLeuAsnValHis100105110ArgHisValTyrIleAsnAspGluLeuIleSerGluGlnAspAspGln115120125GluIleLysAspTyrSerLysHisSerPheValAlaTyrLysLeuVal130135140AspAspLeuValAspThrIleLysAsnSerGluHisProThrLeuLys145150155160IleLeuGlyIleCysGluGlnGluLysLeuAsnMetValGlnSerLeu165170175AlaGluLysLysPheGluGlyLysIleAsnIleMetArgSerPheSer180185190ThrTyrLeuAspPheMetProPheGlyValSerLysGlyGluAlaLeu195200205LysIleLeuSerLysIleTyrAsnPheAsnValGluGluValTyrVal210215220PheGlyAspSerGlnAsnAspIleAspMetLeuArgIleSerArgAsn225230235240SerPheAlaMetGlyAsnAlaGlnLysAspValLysGluValAlaArg245250255TyrValIleProSerAsnAspGluAspGlyValAlaPheAlaIleGlu260265270LysIleLeuSerAspAspLeuAspAsnValAsnIle275280<210>38<211>231<212>PRT<213>Deinococcusaerius<400>38MetThrValProGlnProThrAspProAlaThrValLeuAsnProGlu151015ArgLeuArgGlyValLeuLeuAspValAspGlyThrLeuIleAspSer202530AsnAspAlaHisAlaArgAlaTrpValGluAlaLeuArgGluGluGly354045PheGluArgSerPheGlyGluValArgProLeuIleGlyMetGlyGly505560AspGlnLeuValProArgLeuThrGlyGluAspGlyGluSerGluVal65707580GlyLysArgLeuThrGlnGlyTrpLeuLysHisPheLysProLeuVal859095ProGlyLeuArgProThrArgGlyAsnArgAlaLeuIleGluGlyLeu100105110ArgSerArgGlyLeuLysValValLeuAlaThrSerGlyGluAlaGlu115120125IleValAspSerLeuLeuLysGlnAlaAsnLeuGlyAspLeuAsnLeu130135140AspArgValSerSerSerGluValGlySerSerLysProAlaProAsp145150155160LeuValGlnValGlyLeuAspLysLeuGlyLeuProAlaGlyAlaAla165170175LeuMetValGlyAspThrProPheAspAlaGluAlaAlaHisGlyAla180185190GlyValProCysAlaLeuLeuArgCysGlyGlyAspSerGluGluGlu195200205LeuGlyArgThrGlyAlaLeuValLeuAsnAspProGlnAlaLeuLeu210215220GluAlaLeuGluGlyAlaSer225230<210>39<211>231<212>PRT<213>Deinococcusapachensis<400>39MetThrAlaLeuGlnProAlaAspProAlaAlaAlaLeuAsnProAsp151015ArgLeuArgGlyValLeuLeuAspValAspGlyThrLeuIleAspSer202530AsnAspAlaHisAlaArgAlaTrpValGluAlaLeuArgGluGluGly354045PheGluArgSerPheGlyGluValArgProLeuIleGlyMetGlyGly505560AspGlnLeuIleProArgLeuThrGlyGluAspGlyGluSerGluLeu65707580GlyLysArgLeuThrGlnGlyTrpLeuLysHisPheLysProLeuIle859095ProGluLeuGlnProThrArgGlyAsnArgAlaLeuIleGluGlyLeu100105110ArgAlaArgGlyLeuArgValAlaLeuAlaThrSerGlyGluAlaGlu115120125IleValAspGlyLeuLeuLysGlnAlaGlyLeuAspAspLeuAsnLeu130135140AspArgValSerSerSerGluValGluSerSerLysProGluProAsp145150155160LeuValGluAlaGlyLeuAsnLysLeuGlyLeuProAlaAspAlaAla165170175LeuMetValGlyAspThrProPheAspAlaGluAlaAlaHisLysAla180185190GlyValProCysValLeuLeuArgCysGlyGlyAspSerAspGluGlu195200205LeuGlyArgAlaProAlaLeuValLeuAspAspProGlnAlaLeuLeu210215220GluAlaLeuGluGlyThrSer225230<210>40<211>228<212>PRT<213>Deinococcusaquatilis<400>40MetArgArgAlaGluAspValTyrLeuAspArgMetTyrLysGlyVal151015IlePheAspIleAspGlyThrLeuValAspSerAsnAspAlaHisAla202530ArgAlaTrpValLysAlaPheAlaAspGluHisIleThrValProPhe354045AspGlnValArgProLeuIleGlyMetGlySerAspGlnMetValPro505560ArgLeuThrAspValGlnLysAspSerProThrPheLysArgLeuGly65707580GluAlaTrpLysGlnHisPheGlnAlaGluGluMetProHisLeuLys859095AlaGlnProGlyValArgAlaLeuIleGluArgLeuGlnGluArgGly100105110LeuArgLeuIleValGlyThrSerAlaAspGluAlaLeuValAlaAsp115120125LeuLeuArgValAlaGlyValAspAspLeuLeuThrGluTyrThrThr130135140AlaSerAspValGluSerSerLysProGluProAspIleValGlnAla145150155160AlaValThrLysLeuGlyLeuAlaProAlaGluValLeuMetValGly165170175AspThrProPheAspIleGluSerAlaHisLysAlaGlyValAlaThr180185190ValAlaLeuArgCysGlyGlyAspThrArgPheGluGlyAlaAlaAla195200205ValTyrAlaAspProGlnGluTrpLeuGluGlnLeuAspGlnSerPro210215220LeuGlyLysGly225<210>41<211>229<212>PRT<213>Deinococcusgeothermalis<400>41MetSerHisProGluProThrAlaProAlaAlaAlaLeuAspProLys151015ArgLeuArgGlyValLeuLeuAspValAspGlyThrLeuIleAspSer202530AsnAspAlaHisAlaArgAlaTrpValGluAlaLeuArgGluAlaGly354045PheGluArgThrPheGluGluValArgProLeuIleGlyMetGlyGly505560AspGlnLeuIleProArgLeuThrGlyLeuAspSerGlnSerGluGlu65707580GlyGlnArgLeuThrGlnGlyTrpLeuArgHisPheLysProLeuIle859095ProThrLeuHisAlaThrArgGlyAlaArgAlaLeuValGlnGlyLeu100105110HisAlaArgGlyLeuGlnValLeuLeuAlaThrSerGlyGluAlaGlu115120125IleValAspGluLeuLeuLysGlnAlaHisLeuAspAspLeuGlnLeu130135140GluArgValSerSerSerGluValGluSerSerLysProAlaProAsp145150155160LeuValGlnAlaGlyLeuGluLysLeuGlyLeuProAlaGlyGluAla165170175LeuLeuValGlyAspThrProTyrAspAlaGlnAlaAlaHisLysAla180185190GlyValProCysValLeuLeuArgCysGlyGlyAsnThrGlyLeuGlu195200205GluHisAlaProThrLeuAspAspProGlnAlaLeuLeuGluValLeu210215220GluGlyThrLysGly225<210>42<211>225<212>PRT<213>Deinococcushopiensis<400>42MetSerLeuLysAspLeuLeuSerProSerHisLeuArgGlyValLeu151015LeuAspValAspGlyThrLeuIleAspSerAsnGlyAlaHisAlaArg202530AlaTrpValGlnAlaLeuArgAspGluGlyPheAlaArgThrPheGlu354045AspValArgProLeuIleGlyMetGlyGlyAspLysLeuValProGlu505560LeuThrGlyGluAspProGluGlyGluArgAlaLysArgMetLysAsp65707580AlaTrpLeuLysHisPheGlnProMetIleProLysLeuGlnProThr859095ArgGlyAlaArgGluMetIleGluGlyLeuLeuAlaArgAspLeuArg100105110ValAlaIleAlaThrSerGlyGluAlaGluIleValGluGlyLeuLeu115120125AlaArgValGlyValAlaHisLeuLysLeuAspArgValSerSerSer130135140GluValAspHisSerLysProAspProAspLeuIleGlnValGlyLeu145150155160AsnLysLeuGlyValSerAlaGluGlnAlaLeuMetValGlyAspThr165170175ProPheAspAlaGluAlaAlaArgLysAlaGlyValProSerValLeu180185190LeuArgCysGlyGlyAspAlaArgValGluGlnHisAlaTyrValLeu195200205AspAspProArgAlaLeuLeuGluAlaLeuProAspGlySerGlnThr210215220Val225<210>43<211>214<212>PRT<213>Deinococcusmaricopensis<400>43MetSerIleArgAlaValIleMetAspLeuAspGlyThrLeuValAsp151015SerAsnAspAlaHisAlaArgAlaTrpValAlaAlaLeuArgAspPhe202530GlyIleGluArgThrPheGluAspValArgProLeuIleGlyMetGly354045GlyAspGlnLeuLeuProArgValAlaAspValAspProAsnSerGlu505560ArgGlyGluGlyLeuThrGlnAlaTrpAlaGluHisPheLysProMet65707580IleProGluLeuArgAlaThrArgGlyAlaArgGluLeuLeuGluAla859095LeuHisGlyArgGlyLeuArgValIleLeuGlyThrSerGlyAspSer100105110AspValIleAspProLeuLeuGluHisIleGlyValArgAspLeuVal115120125ProAspArgValThrAlaAspAspValGluAlaSerLysProGluPro130135140AspIleLeuHisAlaAlaLeuGlnLysLeuGlyValGlyAlaHisGlu145150155160AlaLeuMetValGlyAspThrProPheAspAlaGluAlaAlaLysArg165170175AlaAsnValArgValAlaLeuLeuArgAlaGlyGlyAspGluArgVal180185190AsnAlaGluAlaPheThrPheAspAspProAlaAspLeuAlaAlaHis195200205LeuAspAspValLeuArg210<210>44<211>261<212>PRT<213>Deinococcusmurrayi<400>44MetLeuLeuAlaPheAspLeuAspGlyThrLeuIleProAspLeuGly151015ArgGluValProAlaAlaThrValArgAlaLeuAlaArgLeuArgGly202530LeuGlyAlaArgValAlaValIleThrGlyArgAspAlaAlaProPro354045GlnValLeuGluAlaAlaGlnProAspAlaValAlaThrAsnAsnGly505560GlyArgValGluLeuGlnGlyGluLeuHisArgGluAlaArgPheSer65707580GluAspGluLeuAlaAlaValLeuAlaHisGluLeuGluGluAlaArg859095ValValValPheArgProSerGlyLeuTyrAlaGluLeuProProGly100105110HisAlaProGluAlaTrpMetValGluArgGlyValArgProLeuAla115120125GlnAlaProAlaGlyGluProThrLeuLysValGlyPheTyrHisPro130135140GlyValAlaAspPheAlaAlaArgLeuArgValSerHisProHisLeu145150155160ValLeuThrGlyAlaGlnProProTyrSerGluPheLeuThrValThr165170175ProAlaGlyAlaHisLysGlyAlaAlaLeuThrLeuIleAlaGlnGly180185190LeuGlyValGluLeuGluArgThrValValPheGlyAspSerAspAsn195200205AspValAlaMetLeuGluIleAlaGlyTyrAlaValGlnValGlyHis210215220LeuProLeuLeuAlaProHisAlaAspAspArgValSerGlyProGln225230235240AlaLeuGlyProTyrLeuGluGluLeuAlaAspArgLeuGluAlaAla245250255GluAlaProAlaThr260<210>45<211>225<212>PRT<213>Deinococcusmurrayi<400>45MetThrHisHisAspLeuLeuSerProGluArgLeuArgGlyValLeu151015LeuAspValAspGlyThrLeuAlaAspSerAsnHisAlaHisAlaArg202530AlaTrpAlaAlaAlaLeuArgAspGluGlyPheGluLysSerProGlu354045AlaIlePheProLeuIleGlyMetGlyGlyAspLysLeuValProGlu505560LeuThrGlyLeuAspAlaGluSerGluArgGlyGlnArgMetThrGlu65707580GlyTrpValArgHisPheGlnGluLeuIleProAspLeuArgProThr859095ProGlyAlaArgAlaLeuValGluGlyLeuArgAlaArgGlyLeuArg100105110ValValLeuAlaThrSerGlyGluAlaGluValValAspAlaLeuLeu115120125ArgGlnIleGlyLeuAspAspLeuGluLeuGluArgValSerSerThr130135140AspValGluAsnSerLysProAspProAspLeuValGlnAlaGlyLeu145150155160ArgThrLeuGlyLeuProAlaGluAlaThrLeuMetValGlyAspThr165170175ProTyrAspAlaGluAlaAlaArgLysAlaGlyValProCysValLeu180185190LeuArgCysGlyGlyHisProGlyValGluGluHisAspProValTyr195200205AspHisProGlnAlaLeuLeuGluAlaLeuAspGlnAlaPheProVal210215220Glu225<210>46<211>229<212>PRT<213>Deinococcusreticulitermitis<400>46MetAlaAspLeuGlnLeuSerSerProAlaLeuProAlaValSerArg151015LeuLysGlyLeuLeuLeuAspLeuAspGlyThrLeuValAspSerAsn202530GlyAlaHisThrGluAlaTrpValGlnAlaLeuAlaAspGlnGlyPhe354045ThrArgProProAlaGluValArgProLeuIleGlyMetGlyGlyAsp505560GlnLeuValProArgLeuThrGlyLeuAspGluGlySerGluThrGly65707580LysAlaLeuValGluGlyTrpGlnThrHisPheLysSerArgMetPro859095SerValArgThrPheProGlyAlaArgGluLeuLeuGluTrpAlaArg100105110GluValGlyLeuProValValLeuAlaSerSerGlyGluAspGluIle115120125ValGluGlyLeuLeuAlaGlnAlaGlyLeuLeuGluLeuValThrAsp130135140ArgValArgSerAspGluValAsnGlnThrLysProArgProAspVal145150155160ValGlnAlaAlaLeuLysLysAlaGlyValAlaProGluGluAlaLeu165170175PheValGlyAspThrValHisAspAlaArgAlaAlaArgAlaAlaGly180185190ValProCysValLeuValArgAlaGlyGlySerProGlyLeuAspAla195200205GluProHisValLeuGlyAspLeuMetGluLeuLeuAlaAlaLeuLys210215220AlaGlySerThrThr225<210>47<211>227<212>PRT<213>Deinococcuswulumuqiensis<400>47MetArgLeuSerProGlnThrProAlaLeuProAlaValProAspLeu151015ArgAlaLeuLeuLeuAspLeuAspGlyThrLeuAlaAspSerAsnAsp202530AlaHisAlaArgAlaTrpValGlnAlaLeuAlaAspGlnAspIleThr354045ArgGluGluSerGluValArgProLeuIleGlyMetGlyGlyAspGln505560LeuValProAlaLeuThrGlyGluSerAspArgSerGluLeuGlyLys65707580AlaLeuValGlnGlyTrpGlnAspHisPheGlyProMetIleProGlu859095IleAlaProLeuGlyGlyAlaArgGluLeuLeuGluTrpAlaArgThr100105110GlnGlyLeuLysValValLeuAlaSerSerGlyGluAspAspIleVal115120125AspAlaLeuLeuGluGlnIleGlyValGlyAspLeuValProLeuArg130135140ValArgSerAspGluValGlnArgThrLysProAlaProAspValLeu145150155160GlnAlaAlaLeuAlaLysAlaGlyValSerAlaAlaGlnAlaLeuPhe165170175ValGlyAspThrThrTyrAspAlaArgAlaAlaArgAlaAlaGlyVal180185190AlaCysValValLeuArgAlaGlyGlySerProGlyLeuAsnThrGlu195200205ProHisValLeuSerAspProAlaGluLeuLeuAlaGluLeuLysLys210215220AlaThrSer225<210>48<211>224<212>PRT<213>Deinococcussp.Leaf326<400>48MetSerSerTyrLeuGlyValIlePheAspLeuAspGlyThrLeuVal151015AspSerAsnAspGlyHisAlaArgAlaTrpValArgAlaPheAlaAsp202530GlnGlyIleGluValThrPheGlyGlnValArgProLeuMetGlyMet354045GlyGlyAspGlnLeuValProHisLeuThrGlyIleGlyLysAspAsp505560ProArgTyrGluAlaLeuSerAspGlyTrpLysArgHisPheGlnAla65707580GluGluLeuProArgLeuArgAlaGlnProGlyValArgProLeuLeu859095ValAlaLeuGlnAlaGlnGlyLeuArgLeuIleValGlyThrSerAla100105110AspGluAlaLeuValArgAspLeuLeuGlyValAlaGlyAlaGlnAsp115120125LeuPheThrGluTyrThrThrAlaSerGluValGluGluSerLysPro130135140GlnProAspIleValGlnAlaAlaValArgLysLeuGlyLeuAspPro145150155160AlaGlnValLeuMetValGlyAspThrSerPheAspIleGluSerAla165170175ArgGlyAlaGlyValAlaThrValAlaLeuArgSerGlyGlyThrAla180185190GlnPheThrGlyAlaAlaAlaValTyrAspSerProGlnAspTrpLeu195200205AspHisLeuAlaAlaSerProLeuGlyProAspGlnProSerAlaSer210215220<210>49<211>225<212>PRT<213>Deinococcusphoenicis<400>49MetThrProSerLeuGluAlaAlaLeuAsnProAspArgLeuArgGly151015ValLeuLeuAspValAspGlyThrLeuIleAspSerAsnAspAlaHis202530AlaArgAlaTrpValGluAlaLeuArgAspGluGlyPheGluLysThr354045ValGlyGluValArgProLeuIleGlyMetGlyGlyAspGlnMetIle505560ProArgLeuThrGlyLysGlySerGluSerGluValGlyGlnArgLeu65707580ThrAspGlyTrpLeuLysHisPheLysProLeuIleProGluLeuHis859095AlaThrArgGlyAlaArgAlaLeuValGluGlyLeuArgAlaArgGly100105110LeuArgValValLeuAlaThrSerGlyGluAlaGluValValAspAla115120125LeuLeuLysGlnAlaGlyLeuAlaAspLeuAsnLeuAspArgValSer130135140SerSerGluValGluSerSerLysProAlaProAspLeuIleGlnVal145150155160GlyLeuLysLysLeuGlyValProAlaGluAlaAlaLeuMetValGly165170175AspThrProTyrAspAlaGluAlaAlaAlaLysAlaAspValProCys180185190IleLeuLeuArgCysGlyGlyAspThrAspLeuGluLysHisAlaPro195200205ValLeuAspAspProGlnAlaLeuLeuGluAlaLeuGluLysAlaThr210215220Val225<210>50<211>228<212>PRT<213>Deinococcusproteolyticus<400>50MetGlnLeuAsnProGlnGlyProSerLeuProThrIleAlaAspLeu151015ArgAlaLeuPheValAspLeuAspGlyThrLeuValAspSerAsnAsp202530AlaHisAlaArgAlaTrpValGlnAlaLeuAlaAspGlnAspIleThr354045ArgAspGlnGlyGluValArgProLeuIleGlyMetGlyGlyAspGln505560LeuValProGluLeuThrGlyLysSerAspSerSerGluLeuGlyGlu65707580AlaLeuValGlnGlyTrpGlnAspHisPheLysProLeuIleAlaGly859095LeuGluValLeuProGlyAlaArgGluLeuLeuGluTrpAlaArgGly100105110GlnGlyLeuThrValValLeuAlaSerSerGlyGluAspAspIleVal115120125GluAlaLeuLeuGluHisAlaGlyLeuThrAspLeuIleAspLeuArg130135140ValArgSerAspGluValGlnGlnThrLysProGluProAspIleLeu145150155160GlnAlaAlaLeuAsnLysAlaGlyValGlnProGlnGlnAlaLeuPhe165170175ValGlyAspThrArgPheAspAlaGluAlaAlaGlnLysAlaGlyVal180185190ProCysValLeuLeuArgAlaGlyGlySerProAspLeuGluGluAla195200205GlnTyrValLeuAlaAsnAlaHisGluLeuLeuSerAlaLeuGlnAla210215220AlaThrGlnAsp225<210>51<211>216<212>PRT<213>Deinococcussp.17bor-2<400>51MetThrThrSerLeuGlnAlaAlaIleLeuAspLeuAspGlyThrLeu151015IleAspSerAsnAspAlaHisAlaArgAlaTrpValThrSerLeuLys202530ArgHisGlyPheAspIleProPheGluArgValArgProLeuIleGly354045MetGlyGlyAspLysLeuIleProGluLeuThrGlyLeuAspProGlu505560SerGluThrGlyLysAlaLeuThrGlnGlyTrpGlyAspAlaPheLys65707580ProMetIleProSerLeuGlnAlaThrProGlyAlaArgGluLeuVal859095AlaGlyLeuArgGluLeuGlyTrpLysLeuMetLeuGlySerSerGly100105110GluAspGluValValGluGlnGluLeuGluHisLeuGlyLeuGluAsp115120125LeuLysAsnSerArgValThrSerSerGluValGluGluSerLysPro130135140AspAlaAspIleLeuAlaValAlaLeuArgLysLeuGlyValProAla145150155160GlyAlaAlaLeuMetValGlyAspThrLysTyrAspAlaGluAlaAla165170175ArgArgAlaGlyValArgCysValLeuLeuArgCysGlyGlyAsnPro180185190AspLeuProGlyGluValTyrAlaSerProAlaAspLeuLeuGlyAla195200205LeuArgSerGlyAspTrpGlyArg210215<210>52<211>225<212>PRT<213>Deinococcussp.NW-56<400>52MetThrGlnHisAspLeuLeuAlaProAspArgLeuArgGlyValLeu151015LeuAspValAspGlyThrLeuAlaAspSerAsnLeuAlaHisAlaArg202530AlaTrpAlaAlaAlaLeuAlaAspGluGlyPheGluLysThrProGlu354045AspIlePheProLeuIleGlyMetGlyGlyAspLysLeuValProGlu505560LeuThrGlyLeuAspAlaGluSerGluArgGlyGlnArgLeuThrAsp65707580GlyTrpValArgHisPheArgGluLeuIleProAspLeuArgProThr859095ProGlyAlaArgAlaLeuIleGluGlyLeuArgAlaArgGlyLeuArg100105110ValAlaLeuAlaThrSerGlyGluAlaGluIleValAspAlaLeuLeu115120125GluGlnIleGlyLeuAspAspLeuLysLeuGluArgValSerSerSer130135140AspValGluAsnSerLysProAspProAspLeuValLysAlaGlyLeu145150155160HisThrLeuGlyLeuProAlaGlyAlaAlaLeuMetValGlyAspThr165170175ProTyrAspAlaGluAlaAlaArgGlyAlaGlyValProCysValLeu180185190LeuArgCysGlyGlyHisProGlyAlaGluGlyHisAspProLeuTyr195200205AspAspProGlnAlaLeuLeuAlaAlaLeuAsnGluAlaPheProVal210215220Glu225<210>53<211>225<212>PRT<213>Deinococcussp.RL<400>53MetThrGlnGlyAspLeuLeuSerProLysArgLeuArgGlyValLeu151015LeuAspValAspGlyThrLeuAlaAspSerAsnMetAlaHisAlaArg202530AlaTrpAlaAlaAlaLeuArgAspGluGlyPheGluGlnSerProGlu354045AlaIleLeuProLeuIleGlyMetGlyGlyAspLysLeuValProGlu505560LeuThrGlyLeuAspAlaGluSerGluArgGlyGlnArgLeuThrAsp65707580GlyTrpAlaArgHisPheArgAlaLeuIleProAspLeuArgProThr859095ProGlyAlaArgAlaLeuLeuGluGlyLeuArgAlaArgGlyLeuArg100105110ValAlaLeuAlaThrSerGlyGluAlaGluValValAspAlaLeuLeu115120125GlnGlnIleGlyLeuAspAspLeuGluLeuGluArgValSerSerSer130135140AspValGluAsnSerLysProAspProAspLeuValGlnAlaGlyLeu145150155160ArgThrLeuGlyLeuProAlaGluAlaThrLeuMetValGlyAspThr165170175ProTyrAspAlaGluAlaAlaArgLysAlaGlyValProCysValLeu180185190LeuArgCysGlyGlyHisProGlyValGluGluHisAspProValTyr195200205AspHisProGlnAlaLeuLeuGluAlaLeuAspGlnAlaPheProVal210215220Glu225<210>54<211>198<212>PRT<213>Deinococcussp.RL<400>54MetAlaHisAlaArgAlaTrpAlaAlaAlaLeuArgAspGluGlyPhe151015GluGlnSerProGluAlaIleLeuProLeuIleGlyMetGlyGlyAsp202530LysLeuValProGluLeuThrGlyLeuAspAlaGluSerGluArgGly354045GlnArgLeuThrAspGlyTrpAlaArgHisPheArgAlaLeuIlePro505560AspLeuArgProThrProGlyAlaArgAlaLeuLeuGluGlyLeuArg65707580AlaArgGlyLeuArgValAlaLeuAlaThrSerGlyGluAlaGluVal859095ValAspAlaLeuLeuGlnGlnIleGlyLeuAspAspLeuGluLeuGlu100105110ArgValSerSerSerAspValGluAsnSerLysProAspProAspLeu115120125ValGlnAlaGlyLeuArgThrLeuGlyLeuProAlaGluAlaThrLeu130135140MetValGlyAspThrProTyrAspAlaGluAlaAlaArgLysAlaGly145150155160ValProCysValLeuLeuArgCysGlyGlyHisProGlyValGluGlu165170175HisAspProValTyrAspHisProGlnAlaLeuLeuGluAlaLeuAsp180185190GlnAlaPheProValGlu195<210>55<211>223<212>PRT<213>Deinococcussp.YIM77859<400>55MetIleAlaGluGluLeuLeuSerProAspArgLeuArgGlyValLeu151015LeuAspValAspGlyThrLeuValAspSerAsnAspAlaHisAlaTyr202530AlaTrpLeuGluAlaLeuArgGluGluGlyPheThrArgThrPheGlu354045GluValArgProLeuIleGlyMetGlyGlyAspGlnLeuValProArg505560LeuThrGlyGluAspGlyGluSerGluArgGlyLysArgLeuThrArg65707580GlyTrpLeuAsnHisPheLysProArgIleProGlnLeuGlnAlaThr859095ArgGlyAlaArgAlaLeuValGluGlyLeuGlnAlaArgGlyLeuArg100105110ValValLeuAlaThrSerGlyGluAlaAspIleValAspGlyLeuLeu115120125LysArgAlaHisLeuGluAspLeuAsnLeuAspArgValSerSerSer130135140GluValGluSerSerLysProAlaProAspLeuIleGlnAlaGlyLeu145150155160LysLysLeuGlyLeuProAlaGluAlaAlaLeuMetValGlyAspThr165170175ProTyrAspAlaGluAlaAlaArgLysAlaGlyValProTyrValLeu180185190LeuArgCysGlyGlyAsnThrGlyLeuGluGluHisGlyProThrLeu195200205AspAspProGlnAlaLeuLeuGluAlaLeuGluLysAlaSerAla210215220<210>56<211>240<212>PRT<213>粘硫还原球菌(Desulfurococcusmucosus)<400>56MetAlaValLysIleValPheThrAspIleAspGlyThrLeuThrVal151015AsnArgGluSerTyrArgLeuAlaValGluAlaValGluAlaLeuArg202530MetLeuValAspLysGlyValIleValSerLeuValSerSerAsnAla354045LeuProValValValAlaLeuSerArgTyrIleGlyLeuAsnGlyPro505560ValValGlyGluSerGlyAlaLeuValTyrHisAspGluTrpGlyLeu65707580ValGluLeuAlaGlyGluSerAlaArgGlnValTyrMetAspLeuLeu859095GlyAsnTyrArgGluTyrValGluAspSerTrpGlnAsnLysPheArg100105110LeuTyrGluTyrAlaLeuLysLeuArgArgGluHisArgGlyArgAla115120125ArgGluValValAspGluLeuArgArgTyrValGluSerLysTyrLys130135140GlyPheThrValAspTyrSerGlyTyrAlaIleHisValHisAlaLys145150155160GlyValGlyLysGlyValAlaValAspTyrIleLeuArgArgLeuGly165170175LeuSerGlyGlyGluAlaLeuGlyIleGlyAspSerTyrMetAspVal180185190AspPheIleAlaArgLeuGlyTyrArgAlaAlaValGlyGlyAlaAsp195200205GluGluLeuValArgValCysAsnIleValAlaGluAlaProSerGly210215220LeuGlyValAlaGluValValHisArgIleLeuGlyGluArgHisGlu225230235240<210>57<211>230<212>PRT<213>粘硫还原球菌(Desulfurococcusmucosus)<400>57MetAsnSerLeuLeuArgGlyIleArgLeuIleLeuPheAspLeuAsp151015GlyThrIleValAspSerGluAspPheIleValTrpSerPheValGlu202530AlaGlyArgLeuThrGlyIleArgValAspProLeuLysValArgGlu354045LeuIleGlyPheProLeuGluSerIleLeuGluAlaValValGlyThr505560GlyLeuSerArgGluAspAlaAlaArgPheIleGluValArgArgLys65707580LeuValGlnGluAsnTrpPheLysHisValArgLeuPheProAspVal859095IleProValLeuArgGlnLeuSerSerThrGlyTyrMetLeuGlyVal100105110AlaSerSerSerIleArgGluArgValGluLeuPheLeuSerHisLeu115120125GlyValSerGlyTyrPheLysValValSerGlyLeuGluProGlyVal130135140LysGlyLysProGluProAspValIleValAsnAlaLeuLysAlaAla145150155160GlyValProArgSerGluAlaLeuTyrValGlyAspArgMetValAsp165170175CysIleAlaAlaArgArgAlaGlyValLysValValIleValAspArg180185190GlySerValAsnArgTrpLysGlnGluGluCysValProAspAlaTrp195200205IleSerSerLeuLeuGluLeuValAspAspAlaSerThrProThrArg210215220GluAlaThrArgHisAsp225230<210>58<211>224<212>PRT<213>粘硫还原球菌(Desulfurococcusmucosus)<400>58MetArgIleArgLeuValValMetAspTyrAspLeuSerMetValTyr151015AsnLeuSerAspPheTyrGluAlaTyrSerGlyAlaLeuLysSerTyr202530GlyGlyGlyPheIleSerPheAspGluPheLeuAsnLeuLeuAsnGlu354045AspArgLeuSerGluArgIleProGlyGlyValArgGluGluGluPhe505560TrpLeuLeuPheArgArgIleTyrValSerArgHisProIleProMet65707580AspGlyValValAspLeuLeuArgLeuLeuLysSerLeuProValLys859095IleAlaValValSerGlyArgGluThrSerGlyGluTyrIleTrpArg100105110AspLeuArgMetLeuGlyLeuAspGluTyrValAspGluValTyrThr115120125ValSerAspLeuGlnArgLeuAsnGlyLeuGluGluTyrLeuPheAsp130135140LysSerTrpIleIleSerTyrIleLeuArgLysHisGlyValAlaPro145150155160CysAsnAlaLeuCysIleGlyAspPheThrThrAspLeuLeuSerCys165170175ArgLysLeuGlyIleProPheIleGlyValAsnLysAspGluTyrArg180185190GlyMetLeuLeuLysLysArgGlyAlaAlaIleValValLysAsnLeu195200205PheGluValValGlnTrpIleProGluLeuGluLysAspSerThrCys210215220<210>59<211>265<212>PRT<213>嗜热网球菌(Dictyoglomusturgidum)<400>59MetLysArgIleLeuGluValAlaIleLysThrIleLysGluSerGly151015AsnIleLeuLeuAsnTyrIleGlyGluGluLysGluIleGluLeuLys202530GlyIleSerAsnLeuValThrGlnValAspLysLeuSerGluArgHis354045IleLeuLysSerIleGluGluAsnPheProAspHisSerIleLeuThr505560GluGluThrGlyPheIleAsnLysAsnSerGluTyrThrTrpIleVal65707580AspProLeuAspGlyThrThrAsnTyrAlaHisAsnPheProPhePhe859095GlyIleSerIleAlaLeuIleLysAsnLysGluIleIleLeuGlyLeu100105110IleTyrAspProIleArgAspGluLeuPheTyrAlaIleLysAsnGlu115120125GlyAlaTyrLeuAsnAspArgArgIleGluValSerLysThrGluSer130135140LeuGluAsnSerLeuIleSerPheAlaPheProTyrGluLeuSerLeu145150155160GluGluLysAsnPheIleProPheIleAsnPheSerSerArgThrHis165170175GlyIleArgArgThrGlySerAlaAlaIleGluIleAlaTyrValGly180185190CysGlyArgLeuAspGlyPheTrpAlaLysLysLeuLysProTrpAsp195200205IleSerAlaGlyIleLeuIleValGluGluAlaLysGlyLysValThr210215220AspPheSerGlyAsnAsnIleAspIleHisThrAspAsnIleLeuPhe225230235240SerAsnGlyLysIleHisGlnGluMetIleLysIleLeuAsnLeuGly245250255LysIlePheIleArgAsnGluLysPhe260265<210>60<211>249<212>PRT<213>嗜热网球菌(Dictyoglomusturgidum)<400>60MetTyrLysLeuPheIleThrAspLeuAspGlySerIleMetAspGly151015SerGluValIleSerThrLysAsnLeuLysAlaIleLysIleLeuArg202530GluAsnAsnIleGluIleThrIleAlaThrGlyArgArgTrpSerSer354045IleSerLysIleValGluProLeuSerLeuThrLeuProValIleLeu505560TyrAsnGlyAlaGlyIleTyrAspProLeuArgLysSerPheLeuTyr65707580LeuGlnHisLeuSerLysGluGluValIleGlyAlaLeuLysValIle859095HisTyrTyrLeuSerPheValLysLeuGlyIleTyrHisAspAspLys100105110LeuTyrGluAspSerGluAlaLeuGluPheLeuLysAspAsnLysAsn115120125GlyIleIleLysIlePheIleGluGlyGluLysGluLeuLeuLysGlu130135140LeuLysAsnArgLeuGluLysIleTyrLeuAsnValValPheSerSer145150155160TyrLysTyrLeuGluIleLeuProLysGlyAlaSerLysGlyLysAla165170175LeuLysLysLeuLeuLysAsnLeuAspIleLysLeuGluGluValIle180185190AlaLeuGlyAspTyrAspAsnAspGluGluMetLeuLysLeuSerGly195200205LeuGlyIleThrLeuLysAsnAlaSerGluArgLeuLysLysIleAla210215220AspTyrValIleAspAlaSerProSerGluSerValHisTyrIleIle225230235240GlnGlnValLeuAsnPheGluArgArg245<210>61<211>213<212>PRT<213>嗜热网球菌(Dictyoglomusturgidum)<400>61MetGluLeuLysGlyIleIlePheAspTrpAspGlyThrLeuValAsp151015SerPheProAlaCysIleLysAlaThrLysGluIlePheLysLysPhe202530GlyPheIleIleSerGluGluGluTyrArgGluLysPheSerProAsn354045TrpTyrAspIleTyrArgLysHisAsnIleProGluLysTyrTrpLys505560GluIleAspLeuLeuTrpLysAspTyrPheAspTyrSerLeuValLys65707580TrpArgGluGlyAlaValGluAsnLeuThrPheLeuLysLysLeuAsn859095LeuLysIleGlyValValThrAlaSerThrLysGluAspIleGluArg100105110GluLysProTyrLeuAsnProGluGluTyrIleAspGlyTrpIleThr115120125TrpGluAspSerGluLysProLysProAspProLeuProLeuLeuLys130135140IleLeuLysLysLeuSerLeuSerProLeuGluValIleTyrValGly145150155160AspThrLysGluAspIleGluMetSerLysLysLeuAsnIleLeuThr165170175IleGlyValLeuSerProPheThrGlnLysGluLysLeuValSerSer180185190AsnProAsnPheLeuPheAsnAsnLeuPheGluLeuLeuLysPheTrp195200205LysAspLeuPheLeu210<210>62<211>213<212>PRT<213>Effusibacilluspohliae<400>62MetLysTyrProTyrIleLeuPheAspLeuAspGlyThrLeuValAsp151015ThrAsnGluLeuIleLeuGlnSerPheGluHisThrLeuGluHisTyr202530PheProGlyArgTyrSerArgGlnAspValLeuProTyrMetGlyGlu354045ProLeuValLysGlnPheAlaArgTrpAlaProProGluGlnValSer505560GluLeuValGlnAlaTyrArgLysTyrAsnValGluAsnHisAspArg65707580LeuAlaThrIlePheProAsnValProGluValLeuAlaGluLeuLys859095AlaGlnGlyCysArgMetAlaValValThrAsnLysMetArgLeuThr100105110AlaGluArgGlyLeuArgLeuPheGlyLeuAspArgTyrLeuGluThr115120125ValValThrValGluAspThrAspGlnHisLysProHisProAlaPro130135140LeuGlnIleAlaIleGluArgLeuGlyGlyGluProAlaGlyThrLeu145150155160MetValGlyAspSerProTyrAspIleLeuAlaGlyGlnAlaAlaGly165170175ThrAlaThrCysAlaValAlaTrpSerLeuArgGlyCysGluGlyLeu180185190AlaSerTyrLysProAspTyrLeuIleAspGluMetArgGluLeuLeu195200205GluIleValArgGly210<210>63<211>215<212>PRT<213>Fervidobacteriumgondwanense<400>63MetArgProLysAlaCysIlePheAspLeuAspGlyValIleValAsp151015ThrAlaLysTyrHisTyrLeuAlaTrpLysArgLeuAlaLysGluLeu202530GlyPheGluPheThrGluLysAspAsnGluArgLeuLysGlyValSer354045ArgMetGluSerLeuGluIleLeuLeuSerValGlyGlyIleLysIle505560GluAspGlnLysLeuLysGluGlnLeuSerAspLysLysAsnAsnTrp65707580TyrValGluTyrIleSerGlnMetThrLysAspGluIleLeuProGly859095ValGluGluPheLeuArgLysLeuLysAsnAlaGlyIleLysIleAla100105110IleGlySerAlaSerLysAsnThrMetThrIleLeuArgArgIleGlu115120125LeuValAspLeuPheAspSerIleIleAspGlyThrLysIleThrLys130135140AlaLysProAspProGluValPheLeuLysAlaAlaGluGluLeuAsn145150155160ValAspProLysAspCysCysValPheGluAspAlaValAlaGlyIle165170175GluAlaAlaLysArgAlaGlyMetLysValIleGlyValGlyAspHis180185190GluIleLeuLysAspAlaAspArgValIleThrSerPheValGlyHis195200205GlyIleGluLeuValGluPhe210215<210>64<211>305<212>PRT<213>Fervidobacteriumislandicum<400>64MetLysGluTrpArgGluGluGluLeuSerAsnLysSerLysCysLys151015ArgThrPheValPheAspLeuAspGlyThrValLeuAsnSerLysAsn202530GluPheProLysGluThrLysThrLeuValGlnAsnIleValGlyAsn354045GlyAspAsnValValPheAlaThrGlyArgMetHisIleSerAlaLys505560LysLeuLeuAspAsnValPheGlyGluAspIlePheProIleIleSer65707580TyrAsnGlyAlaValIleTyrValProGlyGluGlyPheIleTyrGlu859095LysThrLeuAspIleGluThrAlaTyrLysValIleAspPheLeuArg100105110GluLysAsnIleHisIleGlnThrTyrValAspAspAsnLeuTyrSer115120125GluLysAspAspAspGluIleArgLeuTyrAlaLysHisAlaAspVal130135140ProTyrTyrValValGluAspLeuLysAlaLeuGluHisProProIle145150155160LysMetLeuAlaIleAlaGluGluAlaIleLeuAspSerLeuIleGlu165170175ProLeuLysGluIleValAspGlyLysAlaAsnValPheLysSerPhe180185190ProIlePheLeuAspIleValProAlaAspAlaAsnLysGlyIleAla195200205LeuLysPheLeuAlaAspTyrLeuAsnPheAspLeuGlySerThrIle210215220ValPheGlyAspAsnGluAsnAspIleTyrMetPheLysValAlaSer225230235240LysArgIleAlaValSerAsnAlaValTrpArgLeuLysGluValAla245250255AspPheValSerLysSerAsnGluGluAsnGlyValLeuHisAlaPhe260265270ValHisLeuPheProGluTyrValArgGlyIleAspPheSerSerGly275280285HisThrAsnSerAsnIleValAsnThrThrSerAspAsnLysAlaAla290295300Glu305<210>65<211>278<212>PRT<213>Fervidobacteriumislandicum<400>65MetValLysLeuIleValThrAspLeuAspGlyThrLeuLeuAsnAsp151015GluLysHisIleProLysGluAsnIleGluValLeuArgAlaAlaMet202530GluLysGlyIleHisValSerValAlaThrGlyArgAsnPheTyrSer354045AlaLysProTyrValGluGluLeuGlyLeuAspValProValIlePhe505560GlnAsnGlyAlaPheIleTyrGlnTrpMetGluAsnLysIleIleTyr65707580LysSerAlaLeuGlySerGluIleAlaThrLysValIleGluThrAla859095ArgArgHisLysLeuPheTyrIleLeuTyrArgAspPheLeuGluGlu100105110LysAspMetTyrIleAspMetProTyrThrGlySerPheSerLeuTyr115120125LeuGlyGlnAsnGlnTrpArgLeuAsnIleValAspAspValLeuLys130135140TyrIleLysThrLysAspAlaValAlaGluValAlaLeuValGlyGlu145150155160GluLeuValIleArgGlnAlaIleAsnGluAlaLeuAspGlyLeuGlu165170175SerGlnThrSerValValLysAsnThrThrIleGlyAsnGluThrPhe180185190TyrGluIlePheGlyProAsnSerSerLysGluLeuSerIleLysHis195200205LeuPheGluTyrPheLysValAlaProGluGluThrMetTyrLeuGly210215220AspSerPheAsnAspIleGlyLeuLeuLysMetValGlyTyrProIle225230235240ValMetGluAsnGlyHisAspGluValLysGlnTyrAlaArgTyrIle245250255ThrLysSerAsnAsnGluAlaGlyValAlaTyrAlaValLysLysTrp260265270AlaLeuGlyIleAspAsp275<210>66<211>270<212>PRT<213>Fervidobacteriumnodosum<400>66MetValArgLysThrLysThrPheValPheAspLeuAspGlyThrVal151015LeuThrProLysAsnGluPheProLeuGluThrLysGluLeuIleMet202530AsnIleLeuLysLysGlyAspAsnValValPheAlaThrGlyArgMet354045HisIleSerAlaLysLysLeuLeuAspArgValPheGlyGluAspVal505560PheProIleIleSerTyrAsnGlyAlaValValTyrValProAspLys65707580GlyPheIlePheGluLysThrLeuGluLeuSerThrAlaHisLysVal859095IleAspPhePheArgArgAsnAsnIleHisValGlnThrTyrValGlu100105110AspIleLeuTyrThrGluLysAspAsnGluGluIleLysLeuTyrSer115120125LysHisAlaGluValProTyrThrValValAspAspLeuLysAlaIle130135140HisGlnProProIleLysIleLeuGlyIleGlyAspGlnGluLeuLeu145150155160AspArgLeuIleProAspLeuLysAsnValValGlyAsnSerAlaThr165170175ValPheLysSerPheSerIlePheLeuAspValValProAlaAspAla180185190AsnLysGlyIleAlaLeuArgPheLeuAlaAspTyrLeuLysPheAsp195200205LeuSerGluThrIleValPheGlyAspAsnGluAsnAspIlePheMet210215220PheGluValAlaGlyArgLysIleAlaValSerAsnAlaValGluLys225230235240LeuLysGluValAlaAspPheValSerLysSerAsnGluGluAsnGly245250255ValTyrPheAlaPheThrGlnLeuPheProGluTyrIleVal260265270<210>67<211>276<212>PRT<213>Fervidobacteriumnodosum<400>67MetValLysLeuIleValThrAspLeuAspGlyThrLeuLeuAsnAsp151015AspLysHisIleProAspAspAsnIleIleAlaLeuArgGluAlaMet202530GluLysGlyValHisValSerIleAlaThrGlyArgAsnPheGlySer354045AlaLysArgTyrIleLysGluLeuGlyLeuAspValProValIlePhe505560GlnAsnGlyAlaPheIleTyrGlnTrpMetGluAspLysValIleTyr65707580LysSerAspLeuLysSerGluIleAlaLysLeuIleValGluLysAla859095ArgGluLysGlyLeuPheTyrValValTyrIleAspPheLeuGluGlu100105110LysAspMetTyrIleAspAlaAsnTyrSerGlyGluPheLeuSerTyr115120125LeuLysGlnAsnGluTrpArgIleAsnTyrValSerAspValValAsn130135140TyrIleSerAsnArgAspSerIleAlaGluValAlaLeuValGlyAsp145150155160GluGluLysIleLysAsnIleValGluAspAspLeuPheIlePheGly165170175GluSerValSerValValLysAsnAsnArgIleAsnSerGluValPhe180185190TyrGluPhePheGlyProAsnSerSerLysAspIleSerPheAsnTyr195200205LeuLeuLysTyrPheAsnValLysProGluGluThrMetTyrLeuGly210215220AspAsnTyrAsnAspIleGlyMetLeuLysIleValGlyTyrProVal225230235240ValMetGluAsnAlaProAspGluValLysLysTyrAlaLysTyrVal245250255SerLysSerAsnAsnGluAlaGlyValAlaTyrAlaValArgLysLeu260265270ValLeuGlyTyr275<210>68<211>281<212>PRT<213>Fervidobacteriumpennivorans<400>68MetSerAsnIleArgLysLeuLysAsnAlaLysThrPheValPheAsp151015LeuAspGlyThrValLeuAsnSerLysAsnGluPheProGlnGluThr202530LysGlnLeuIleLeuAsnIleLeuGluAsnGlyAspAsnValIlePhe354045AlaThrGlyArgMetHisIleSerAlaLysLysLeuLeuGluAsnVal505560PheGlyGluAspIlePheProIleIleSerTyrAsnGlyAlaValVal65707580TyrValProAsnValGlyPheIleTyrGluLysThrLeuAspMetGlu859095ThrValHisLysValIleGlyPheLeuArgGlnArgAsnIleHisVal100105110GlnThrTyrValAspAspLysLeuTyrSerGluLysAspAsnGluGlu115120125IleLysLeuTyrAlaLysHisAlaAspValProTyrTyrValValAsn130135140AspLeuLysAlaIleGluHisProProIleLysMetLeuAlaIleAla145150155160GluGlnGlyIleLeuAspAsnLeuIleGluProLeuLysGluIleVal165170175AspGlyArgAlaAsnValPheLysSerPheProIlePheLeuAspIle180185190ValProAlaAspAlaAsnLysGlyAsnAlaLeuLysPheLeuAlaAsp195200205TyrLeuGlyPheAspLeuLysAsnThrValValPheGlyAspAsnGlu210215220AsnAspIleTyrMetPheGluValAlaGlyThrLysValAlaValGlu225230235240AsnAlaValAspLysLeuLysGluValAlaAspPheValSerLysSer245250255AsnGluGluAsnGlyValLeuTyrSerPheValArgLeuPheProGlu260265270TyrLeuArgGlyIleGluSerSerAsn275280<210>69<211>278<212>PRT<213>Fervidobacteriumpennivorans<400>69MetValLysLeuIleValThrAspLeuAspGlyThrLeuLeuAsnAsp151015GluLysHisIleProArgGluAsnIleGluAlaLeuLysAlaAlaMet202530AspLysGlyIleHisValSerValAlaThrGlyArgAsnPheTyrSer354045AlaLysProTyrValGluLysLeuGlyLeuAspValProValIlePhe505560GlnAsnGlyAlaPheIleTyrGlnTrpMetGluAspLysValIleTyr65707580LysSerGluLeuLysThrGluIleAlaGluArgValIleGluThrAla859095ArgLysTyrLysIlePheTyrIleLeuTyrArgAspPheLeuGluGlu100105110LysAspMetTyrIleAspGlnProTyrSerGlyAsnPheSerLeuTyr115120125LeuAsnGlnAsnArgTrpArgLeuAsnIleValAspAspValLeuLys130135140TyrLeuLysAsnLysLeuThrValAlaGluValAlaLeuValGlyAsn145150155160GluGluArgIleMetLeuAlaLeuAsnGluSerLeuGlyGluValArg165170175AsnAlaThrSerIleValLysAsnAsnThrIleAsnGluGluThrPhe180185190TyrGluValPheGlyProAsnAlaSerLysGluLeuSerIleLysTyr195200205LeuPheGluTyrPheAsnValAlaProGluGluThrMetTyrLeuGly210215220AspSerPheAsnAspIleGlyLeuLeuLysMetValGlyTyrProVal225230235240ValMetGluAsnGlyHisSerGluValLysGlnTyrAlaArgTyrIle245250255ThrLysSerAsnAsnGluAlaGlyValAlaTyrAlaValArgLysTrp260265270AlaLeuGlyIleAspGlu275<210>70<211>215<212>PRT<213>Fervidobacteriumpennivorans<400>70MetLysProLysAlaCysIlePheAspLeuAspGlyValIleValAsp151015ThrAlaLysTyrHisTyrLeuAlaTrpLysArgLeuAlaLysGluLeu202530GlyPheGluPheThrGluLysAspAsnGluArgLeuLysGlyValSer354045ArgMetGluSerLeuGluIleLeuLeuSerValGlyGlyIleLysIle505560AspAspGluAsnValLysLeuGlnLeuAlaGluLysLysAsnLysTrp65707580TyrValGluTyrIleAsnGlnMetThrLysAspGluIleLeuProGly859095ValIleGluPheLeuGluLeuLeuLysSerAlaGlyIleLysValAla100105110IleGlySerAlaSerLysAsnThrIleThrIleLeuGluArgIleGly115120125LeuLysAspPhePheAspValIleIleAspGlyThrLysIleSerLys130135140AlaLysProAspProGluValPheLeuLysAlaAlaGluGluLeuSer145150155160ValArgProGluGluCysCysValPheGluAspAlaValAlaGlyIle165170175GlnAlaAlaLysSerAlaGlyMetLysValIleGlyValGlyAspPro180185190ThrIleLeuLysAspAlaAspLysValIleGlnSerPheGlnGlyGln195200205ThrLeuGlyLeuIleGluPhe210215<210>71<211>222<212>PRT<213>Geobacillussp.<400>71MetThrIleArgThrIleLeuPheAspLeuAspGlyThrLeuIleAsp151015ThrAsnGluLeuIleIleGlnSerPheLeuHisThrLeuGluLysTyr202530TyrProGlyArgTyrGlyArgGluAspValLeuProPheIleGlyPro354045SerLeuTyrGluThrPheSerSerLeuAspProGluArgAlaGluGlu505560MetValLysThrTyrArgThrPheAsnHisAlaArgHisAspGluLeu65707580IleArgGluPheAspThrValTyrGluThrIleGluThrLeuHisArg859095HisGlyPheArgLeuGlyValValThrThrLysMetHisAspThrAla100105110LeuMetGlyLeuArgLysThrArgLeuGluProPhePheSerCysVal115120125IleGlyLeuAspAspValSerArgProLysProAspProGluProIle130135140HisLysAlaLeuGluAlaLeuGlnSerLysSerAspGluAlaLeuMet145150155160ValGlyAspAsnTyrHisAspIleLeuAlaGlyLysAsnAlaGlyVal165170175LysThrAlaGlyValAlaTrpAlaIleLysGlyArgGlyTyrLeuGlu180185190GlnTyrGluProAspTyrMetLeuGluLysMetSerAspLeuLeuAla195200205IleValGlyIleAsnGluArgLysGluGluThrSerSerGln210215220<210>72<211>264<212>PRT<213>嗜热脂肪地芽孢杆菌(Geobacillusstearothermophilus)<400>72MetAlaGluLysTrpGluGluIleAspArgTyrAlaArgGlnTrpIle151015AspGluAlaGlyLysArgIleArgAlaSerPheAlaLysGlnLeuThr202530ValGluAlaLysGluAsnProAsnAspLeuValThrAsnValAspArg354045AlaIleGluGlnPhePheAlaGluHisIleArgArgGlnPheProSer505560HisArgLeuLeuGlyGluGluGlyPheGlyAspArgIleAspAlaLeu65707580AspGlyValValTrpValIleAspProIleAspGlyThrMetAsnPhe859095ValHisGlnArgArgHisPheAlaValSerIleGlyIlePheGluAsp100105110GlyIleGlyGlnLeuGlyTyrValTyrAspValValPheAspGluLeu115120125TyrAlaAlaGlnLysGlyArgGlyValPheLeuAsnGlyGluProLeu130135140GlyLeuLeuGlnProAlaProValAlaGluSerIleIleAlaIleAsn145150155160GlyThrTrpLeuMetGluAsnLysArgLeuAspHisArgProLeuMet165170175ArgLeuAlaLysGluAlaArgGlyThrArgSerTyrGlySerAlaAla180185190LeuGluLeuAlaTyrValAlaAlaGlyArgLeuAspAlaTyrIleSer195200205ProArgLeuSerProTrpAspPheAlaGlyGlyMetIleLeuIleGlu210215220GluAlaGlyGlyMetValThrThrLeuAspGlyLysProLeuAspLeu225230235240LeuGlyArgAsnSerValLeuAlaAlaLysProGlyValHisGluGlu245250255IleLeuArgArgTyrLeuHisAsp260<210>73<211>283<212>PRT<213>嗜热脂肪地芽孢杆菌(Geobacillusstearothermophilus)<400>73MetLeuIleAlaLeuAspMetAspGlyThrLeuLeuAsnGlyGluGly151015LysIleSerGluArgAsnArgLysAlaMetMetAlaAlaGlnLysGlu202530GlyHisIleValAlaValIleThrGlyArgAlaArgLysAspAlaLeu354045AlaProLeuArgGluAlaGlyLeuValCysProIleAlaSerLeuAsn505560GlyAlaIleValThrLeuGluAspGlyThrValIleGlyGluAlaPro65707580LeuAlaArgThrAlaValArgProThrLeuGluTrpValArgLysGln859095SerAspLeuTyrCysGluThrTyrThrGlyAspAlaValTyrValGly100105110LeuHisAsnArgAlaGlnTrpGluSerLeuAlaSerGluMetAlaAsp115120125AlaAlaProAspValLysTrpLeuValAsnLysGlnPheGlnGlnAla130135140ArgValThrTyrValAspAspIleArgThrValTrpAspAspProGln145150155160LeuThrLeuTyrLysLeuLeuIlePheAlaLeuAspArgGluArgLeu165170175ArgAspAlaAlaSerArgPheAlaAlaLeuProSerValThrValThr180185190SerSerHisProHisAsnIleGluMetAsnAsnGluArgAlaThrLys195200205GlyGluAlaLeuLysGlnLeuAlaAlaHisTyrGlyIleAspLeuCys210215220AspThrValAlaPheGlyAspSerHisAsnAspLeuSerMetPheGlu225230235240ValAlaGlyCysArgValAlaMetAlaAsnAlaAlaProGluLeuLys245250255AlaLysAlaAspIleValThrCysSerHisGluGluAspGlyValAla260265270AlaGlyLeuGluArgValLeuAlaLysArgVal275280<210>74<211>268<212>PRT<213>嗜热脂肪地芽孢杆菌(Geobacillusstearothermophilus)<400>74MetLysProTyrLeuIleAlaLeuAspLeuAspGlyThrLeuLeuLys151015GluAspLysThrIleSerProPheThrLysAspValIleArgArgAla202530IleAspThrGlyHisPheValValIleAlaThrGlyArgProTyrArg354045AlaSerSerMetTyrTyrGluGluLeuGlyLeuAlaThrProIleVal505560AsnPheAsnGlyAlaPheValHisHisProArgGlnProSerTrpGly65707580MetHisHisTyrProLeuProLeuAlaValValLysAspIleValGlu859095IleSerGluSerTyrGlyIleLysAsnMetMetAlaGluValLeuAsp100105110AsnValTyrPheHisGlnHisAspGluValLeuLeuAspIleValArg115120125LeuGlyAsnProThrValGluIleGlyAspLeuArgArgSerLeuGly130135140LysAspProThrSerValLeuValTyrThrAspAspAspHisIleGlu145150155160ArgIleGlnSerHisLeuAlaAsnValTyrAlaAsnValIleHisGln165170175ArgArgTrpSerGluProTrpHisValIleGluIleIleArgHisGly180185190ValHisLysAlaAlaGlyLeuLysGlnValAlaAspTyrPheGlyIle195200205ProArgGluArgValIleAlaPheGlyAspGluAspAsnAspLeuGlu210215220MetIleAspTrpAlaGlyLeuGlyValAlaMetGlyAsnAlaIleGlu225230235240ProLeuLysThrIleAlaAspAspValAlaLysThrAsnGluGluAsp245250255GlyValGlyValTyrLeuGlnAspLeuLeuArgLeu260265<210>75<211>287<212>PRT<213>嗜热脂肪地芽孢杆菌(Geobacillusstearothermophilus)<400>75MetTyrLysLeuLeuAlaLeuAsnIleAspGlyThrIleLeuLysAsn151015AsnGlyArgLeuProArgGluThrLysGluAlaValAspTyrValLys202530LysLysGlyValTyrValThrLeuIleThrSerArgAsnLeuLeuSer354045AlaArgLysValAlaLysAlaLeuArgLeuAspGlyMetLeuIleAla505560PheGlnGlyAlaMetIleAlaArgThrLeuAspAspArgLeuPheAsp65707580AlaValIleProGluGluArgThrPheAsnIleValGlnIleLeuGlu859095AsnPheAsnCysAsnIleArgLeuMetHisGluArgTyrSerLeuGly100105110AsnArgLysLysValLysLysAsnLeuValValGlnThrValLeuSer115120125SerSerAspProPhePheTyrProThrGlnPheValAspSerLeuGly130135140AspValLeuMetAspGluProIleAlaValProLysIleAspValTyr145150155160PheAlaThrAspGluGluArgAspAlaAlaAlaAlaLeuLeuThrLys165170175SerIleProSerIleAspMetIleMetGlnProAsnGlyLysMetGlu180185190IleValProGlnGlyValSerLysLeuAlaGlyLeuArgArgLeuVal195200205GlnHisIleGlyValSerLeuLysGluThrValMetIleGlyAspGly210215220LeuAspAspLeuProAlaIleGluAlaAlaGlyLeuGlyValAlaMet225230235240GlyAsnAlaProLeuGluValLysArgAlaAlaAspTrpValThrArg245250255SerAsnGluGlnLeuGlyValAlaTyrMetValLysGluHisPheArg260265270LysGlnGlnArgIleGluPheLeuGlnLysLeuLysThrLysGln275280285<210>76<211>258<212>PRT<213>嗜热脂肪地芽孢杆菌(Geobacillusstearothermophilus)<400>76MetGlyArgLysIleValPhePheAspIleAspGlyThrLeuLeuAsp151015GluGlnLysGlnLeuProLeuSerThrIleGluAlaValArgArgLeu202530LysGlnSerGlyValTyrValAlaIleAlaThrGlyArgAlaProPhe354045MetPheGluHisValArgLysGlnLeuGlyIleAspSerPheValSer505560PheAsnGlyGlnTyrValValPheGluGlyAsnValLeuTyrLysGln65707580ProLeuArgArgGluLysValArgAlaLeuThrGluGluAlaHisLys859095AsnGlyHisProLeuValPheMetAspAlaGluLysMetArgAlaSer100105110IleSerAspHisProHisIleHisValSerMetAlaSerLeuLysPhe115120125AlaHisProProValAspProLeuTyrTyrGluAsnLysAspIleTyr130135140GlnAlaLeuLeuPheCysArgAlaGluGluGluGluProTyrValArg145150155160AsnTyrProGluPheArgPheValArgTrpHisAspValSerThrAsp165170175ValLeuProAlaGlyGlySerLysAlaGluGlyIleArgMetMetIle180185190GluLysLeuGlyIleAspLysLysAspValTyrAlaPheGlyAspGly195200205LeuAsnAspIleGluMetLeuSerPheValGlyThrGlyValAlaMet210215220GlyAsnAlaHisGluGluValLysArgValAlaAspPheValThrLys225230235240ProValAspLysGluGlyIleTrpTyrGlyLeuLysGlnLeuGlnLeu245250255IleArg<210>77<211>237<212>PRT<213>Halococcussalifodinae<400>77MetAsnArgAlaAspAlaAlaProGluAlaArgProProLeuAlaVal151015AspIleAspGlyThrLeuThrAspArgAsnArgValValAspProArg202530ValPheAspAlaLeuArgGluTrpProAlaProValValLeuAlaThr354045GlyLysAlaLeuProTyrProValAlaLeuCysGluPheAlaGlyLeu505560AlaThrLeuValValAlaGluAsnGlyGlyValValCysLeuAspThr65707580GluThrArgAspAspIleValValAspGlyAspArgThrAlaAlaAsp859095ArgValAlaAlaAlaTyrArgAspAlaGlyHisAspLeuGlyTrpGly100105110SerLeuAspLeuValAsnArgTrpArgGluThrGluIleAlaValSer115120125ArgAspAlaProLeuAspProLeuArgGluIleAlaValGluTyrGly130135140LeuAspValValAspThrGlyPheAlaTyrHisValValSerProAsp145150155160ValAspLysGlyArgGlyLeuSerIleValAlaAspArgLeuGlyArg165170175AspProGluGluPheValAlaIleGlyAspSerAlaAsnAspValAla180185190ThrPheGluValValGlyArgSerPheAlaValAlaAsnAlaAspGlu195200205AlaAlaThrGlnAlaAlaAspLeuValThrAspAlaAlaTyrAlaAsp210215220GlyPheIleGluAlaLeuSerThrValArgSerSerSer225230235<210>78<211>237<212>PRT<213>Halococcussalifodinae<400>78MetAsnArgAlaAspAlaAlaProGluAlaArgProProLeuAlaVal151015AspIleAspGlyThrLeuThrAspArgAsnArgValValAspProArg202530ValPheAspAlaLeuArgGluTrpProAlaProValValLeuAlaThr354045GlyLysAlaLeuProTyrProValAlaLeuCysGluPheAlaGlyLeu505560AlaThrLeuValValAlaGluAsnGlyGlyValValCysLeuAspThr65707580GluThrArgAspAspIleValValAspGlyAspArgThrAlaAlaAsp859095ArgValAlaAlaAlaTyrArgAspAlaGlyHisAspLeuGlyTrpGly100105110SerLeuAspLeuValAsnArgTrpArgGluThrGluIleAlaValSer115120125ArgAspAlaProLeuAspProLeuArgGluIleAlaValGluTyrGly130135140LeuAspValValAspThrGlyPheAlaTyrHisValValSerProAsp145150155160ValAspLysGlyArgGlyLeuSerIleValAlaAspArgLeuGlyArg165170175AspProGluGluPheValAlaIleGlyAspSerAlaAsnAspValAla180185190ThrPheGluValValGlyArgSerPheAlaValAlaAsnAlaAspGlu195200205AlaAlaThrGlnAlaAlaAspLeuValThrAspAlaAlaTyrAlaAsp210215220GlyPheIleGluAlaLeuSerThrValArgSerSerSer225230235<210>79<211>183<212>PRT<213>Halococcussalifodinae<400>79MetThrValThrIleProAspAspThrGluAlaValValTyrAspLeu151015AspGlyThrLeuValArgLeuAlaValAspTrpAspAlaAlaAlaThr202530AspAlaAlaAlaAlaLeuAspGlyCysGlyValAspThrAspGlyMet354045AspLeuTrpArgMetLeuGluArgAlaAspGluLeuGlyHisArgAsp505560AlaValGluGluAlaIleAlaGlyHisGluArgGluGlyAlaArgArg65707580SerGluArgLeuAlaPheAlaAspGluLeuValThrAspArgProVal859095GlyValCysSerLeuAsnCysGluAspAlaCysArgIleAlaLeuAsp100105110ValHisAspLeuAlaGluHisValAlaCysValValGlyArgAspThr115120125ValAlaThrPheLysProAspProGluSerLeuLeuAlaAlaValGlu130135140HisLeuAspAlaThrProGluArgThrLeuPheIleGlyAspSerGlu145150155160GlyAspAlaGluThrAlaAspArgAlaGlyThrArgPheSerTyrVal165170175GlyAspGlyProThrThrTyr180<210>80<211>212<212>PRT<213>Hydrogenivirgasp.128-5-R1-1<400>80MetLysAlaValLeuPheAspLeuAspGlyThrLeuIleAspSerAla151015AspAspIleAlaLeuAlaLeuArgLeuThrLeuGluGluLeuGlyMet202530GluGluLysMetProProSerValArgSerLeuIleGlyGlyGlyVal354045LysAlaLeuLeuGluArgValLeuGlyAspAspPheArgGluGlnHis505560ValLysValPheArgArgHisTyrIleGlyAsnProValValAsnThr65707580ThrProTyrProGlyValMetGluThrLeuArgAlaLeuArgGlyArg859095GlyIleAsnLeuValValValThrAsnLysLeuGluGluLeuSerVal100105110GluIleLeuLysArgLeuGlyMetLeuGluPhePheAspLeuValVal115120125GlyGlyAspThrPheProGluLysLysProSerProLeuProValLeu130135140LysSerLeuGluPheValGlyValGluSerSerGlnAlaLeuMetVal145150155160GlyAspThrSerAlaAspIleGluAlaGlyLysArgAlaGlyValArg165170175ThrAlaLeuAlaAlaTrpGlyTyrValLysLeuAsnSerValLysPro180185190AspTyrIlePheAsnSerProAlaAspIleLeuSerLeuAlaSerGln195200205ValSerGlyAla210<210>81<211>211<212>PRT<213>嗜氢产氢杆菌(Hydrogenobacterhydrogenophilus)<400>81MetArgLeuLysLeuPheIlePheAspLeuAspGlyThrLeuIleAsp151015SerTyrMetAspIleGlyMetCysValAsnMetValLeuGlnGluMet202530GlyArgAsnProIleAspProGluArgValLysAlaTrpIleGlyGly354045GlyAlaArgArgLeuLeuGluLysLeuPheProAspGluGluLeuAsn505560ThrAlaLeuGluLeuPheArgArgPheTyrArgGluAsnProValVal65707580HisThrLysThrTyrGluGlyIleGlnGluValLeuAlaTyrLeuArg859095SerArgGlySerPheLeuAlaValValThrAsnLysMetGluAspLeu100105110SerArgGluIleLeuArgArgLeuGluLeuLeuSerTyrPheAspVal115120125ValValGlyGlyAspThrPheProGluLysLysProSerProLeuPro130135140IleLysGlnValMetGluArgLeuArgValArgProGlnGluSerVal145150155160IleValGlyAspThrSerAlaAspIleCysAlaGlyLysAspAlaGly165170175IleTrpThrAlaLeuAlaLysTrpGlyTyrValArgLeuAspSerVal180185190MetProAspPhePheLeuSerGluProLysAspValIleAspLeuPhe195200205GluLysVal210<210>82<211>262<212>PRT<213>嗜氢产氢杆菌(Hydrogenobacterhydrogenophilus)<400>82MetAspLysValGluSerPheLeuLysValAlaLysGluSerAlaLeu151015IleGlyGlyLeuIleLeuLysGluHisPheGlyLysLeuThrGlnLys202530AspValGlnGluLysSerGluLysAspValValSerPheValAspLys354045SerSerGluGluArgIleArgLysTyrIleLysIleAsnPheProAsp505560HisAspValValGlyGluGluGluGlyGlyThrAspAsnSerAspTyr65707580ValTrpTyrIleAspProLeuAspGlyThrLysAsnTyrIleAlaGly859095PheProIlePheGlyValSerValGlyLeuThrTyrArgLysGluPro100105110IleValGlyAlaValTyrMetProLeuTyrAspThrLeuTyrTrpAla115120125TyrLysGlyGlyGlyAlaTyrLysAsnGlyLysSerIleGluValSer130135140LysArgValGlnValLysSerPhePheIleAlaTyrGlyPheProSer145150155160ArgAlaLysArgAsnLeuAspIleTyrTrpAsnIlePheLysGluLeu165170175PheGluLysValGlyAlaMetArgArgProGlyAlaAlaAlaIleAsp180185190LeuCysPheThrAlaGluGlyIlePheAspGlyLeuIleGluPheGlu195200205LeuAsnProTrpAspValCysAlaGlyIleLeuIleLeuGlnGluAla210215220GlyGlyLysValHisLeuThrLysAspLeuThrLysGlyThrAspVal225230235240IleAlaGlyThrProThrAlaPheProTyrIleGluAsnThrValLys245250255SerAsnLeuGluGlyLeu260<210>83<211>211<212>PRT<213>嗜氢氢杆菌(Hydrogenobacterthermophilus)<400>83MetArgLeuLysLeuPheIlePheAspLeuAspGlyThrLeuIleAsp151015SerTyrMetAspIleGlyMetCysValAsnGlyValLeuArgSerMet202530GlyArgGlnGluIleGluProAspSerValLysAsnTrpIleGlyGly354045GlyAlaArgGlyLeuLeuGluLysLeuPheProSerGluGluIleAsp505560ArgAlaLeuGluLeuPheArgLysTyrTyrArgGluAsnProValVal65707580TyrThrLysAlaTyrLysGlyIleArgGluValLeuThrHisLeuLys859095AlaMetGluLysArgLeuAlaValValThrAsnLysMetGluAspLeu100105110SerValGluIleLeuLysArgLeuAsnLeuTyrHisTyrPheAspMet115120125ValValGlyGlyAspThrPheProGluLysLysProSerProValPro130135140IleLysGluValLeuArgTrpLeuGlyValAspAlaLysGluAlaVal145150155160MetIleGlyAspThrAspAlaAspValArgAlaGlyLysAspAlaGly165170175ValTrpThrAlaLeuAlaAsnTrpGlyTyrValArgMetAspGlyVal180185190LysProAspPheValLeuHisLysProGluAspIleIleAsnLeuPro195200205GlyArgAla210<210>84<211>247<212>PRT<213>丁酸栖高温菌(Hyperthermusbutylicus)<400>84MetAlaGluAlaValArgIleGluLeuProAlaArgValCysGlyLeu151015AlaLeuAspIleAspGlyThrLeuThrGluLysLysArgLeuGlyGlu202530PheAsnIleSerLeuGluAlaValGluAlaValArgArgAlaGluAla354045AlaGlyIleProValMetLeuValThrGlyAsnSerValTyrValVal505560AlaGlyValAlaArgTyrIleGlyAlaSerGlyProHisValAlaGlu65707580AsnGlyCysIleValTyrAspAsnGlyThrIleTyrArgValCysArg859095AspThrAlaArgArgAlaAlaArgLeuIleGluGluGluLeuAlaGly100105110ValLeuLysProSerTrpGlnAsnProCysArgLeuHisAspTyrAla115120125PheIleProLysLeuLeuGluProGlyGluAlaLeuGluAlaValLys130135140ArgValLeuSerSerArgGlyIleAsnValLysValGlyPheSerGly145150155160TyrAlaIleHisLeuArgProValAspAlaSerLysGlyArgGlyLeu165170175LysLeuAlaLeuLysLeuArgGlyLeuGluProAspCysValValAla180185190ValGlyAspSerValIleAspLeuGluMetLysAspAlaGlyValIle195200205LeuAlaAlaValGlyAsnAlaAspGluLysLeuLysLysAsnAlaAsp210215220IleValLeuProGlyGluSerGlyArgSerValLysAlaLeuIleAsp225230235240AlaIleLeuSerAsnArgGly245<210>85<211>267<212>PRT<213>Kosmotogaarenicorallina<400>85MetLysMetIleGluAlaPheValIleAspIleAspGlyThrPheIle151015AspSerLysGlyAsnIleProGlnGluAsnLeuAspAlaLeuLysGlu202530IleGluGluGluGlyIleMetIlePhePheAlaSerGlyArgMetLeu354045SerSerValIleLysPheGlnSerSerLysLeuAsnLysIleHisPro505560IleIleAlaTyrAsnGlyAlaValValTrpAsnGlyArgAspValIle65707580PheSerLysAsnIleThrThrGluMetAlaLysAspIleValSerTyr859095SerLeuSerLysGlyLeuTyrIleGlnAlaTyrValAsnAspGluLeu100105110ValValProPheAspCysGluLysAlaArgGluTyrAlaLysHisSer115120125AlaValAspPheArgValGluGluLysPheIleGluPheValThrLys130135140AsnThrProThrLysLeuLeuIleIleAspThrProGluLysValGlu145150155160ValLeuSerAlaHisPheAlaAlaArgTyrAsnHisLeuAsnValPhe165170175ArgSerPheAlaThrTyrLeuAspLeuLeuProGluGlyValAsnLys180185190GlyLeuGlyLeuLysTyrLeuC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laLeuAspAlaAlaTyrLeu151015AlaLysGlyIleHisGlnTyrTyrGlnGluLysGlyPheThrLeuSer202530SerLysSerThrProThrAspLeuValThrGlnAlaAspHisGluSer354045GluAlaAlaIleArgGluLeuIleAlaAlaArgHisProAspHisVal505560ValLeuGlyGluGluGlnGlyGlnAspArgGluGlyAlaPheArgTrp65707580IleValAspProLeuAspGlyThrValAsnTyrAlaHisGlyPhePro859095PheTyrAlaValSerIleGlyLeuGluAlaHisGlyGluValValLeu100105110GlyValIleLeuAspThrAlaArgGlyAspLeuPheThrAlaThrLys115120125GlyGlyGlyAlaPheMetAsnGlyArgProIleArgValSerProArg130135140AlaThrLeuValGlySerLeuLeuAlaThrGlyPheProTyrAspVal145150155160SerLysAspThrGluAsnLeuValTyrPheGlnArgAlaLeuThrArg165170175GlyLeuMetValArgArgProGlyAlaAlaAlaLeuAspLeuAlaTyr180185190ValAlaAlaGlyArgLeuAspGlyPheTrpGluValLysLeuAsnPro195200205TrpAspValAlaAlaGlyTrpLeuIleIleArgGluAlaGlyGlyThr210215220ValThrGlyMetGluGlyGluAlaTyrArgLeuGlyAsnArgTyrLeu225230235240ValAlaSerAsnGlyLeuIleHisGlnProLeuLeuAspThrIleHis245250255GlyArg<210>92<211>216<212>PRT<213>Meiothermuscerbereus<400>92MetLysLeuLysAlaLeuLeuPheAspLeuAspGlyThrLeuAlaAsp151015ThrAspArgLeuHisGluGlnAlaTrpLeuGluGlyLeuSerArgTyr202530GlyLeuGlnGlyAspHisThrPheTyrGlnThrGlnIleSerGlyGly354045LeuAsnProGluIleValGlnArgLeuLeuProGlnLeuSerGlnAla505560GluAlaGluAlaPheLeuGluGlnLysGluAlaArgPheArgGluLeu65707580AlaSerGluValGlnProLeuProGlyLeuGlyThrLeuTrpAspTrp859095AlaGlnGluGlnAsnLeuArgArgAlaLeuValSerAsnAlaProArg100105110GluAsnAlaGlnTyrLeuLeuLysArgLeuGlyLeuValPheAspHis115120125IleValLeuSerGluGluLeuProAlaGlyLysProAspProLeuPro130135140TyrArgThrAlaLeuGlnAlaLeuAsnIleGlyProSerGluAlaLeu145150155160AlaPheGluAspSerProSerGlyValArgSerAlaValGlyAlaGly165170175IleArgThrValAlaLeuThrThrGlyHisProAlaHisAlaLeuGlu180185190GlnAlaGlyAlaPheLeuCysIleProAsnPheAlaAspProArgLeu195200205TrpAlaTyrLeuHisLysMetGly210215<210>93<211>258<212>PRT<213>Meiothermuschliarophilus<400>93MetAspLeuArgProPheLeuGluThrAlaLeuAlaAlaAlaTyrVal151015AlaArgGlyIleHisGlnLeuTyrGlnAspLysGlyPheThrGlnSer202530ThrLysSerThrProThrAspValValThrGlnAlaAspLysGluAla354045GluAlaAlaIleArgAlaLeuIleGluGlnArgHisProGlyHisVal505560ValLeuGlyGluGluGluGlyGlnGlnGlyGluGlyGluTyrArgTrp65707580IleValAspProLeuAspGlyThrValAsnTyrAlaHisGlyPhePro859095PheTyrCysValSerIleGlyLeuGluValArgGlyGluValMetVal100105110GlyValValLeuAspThrAlaArgGlyAspLeuPheThrAlaThrArg115120125GlyGlyGlyAlaTyrPheAsnGlyArgProMetArgValSerArgSer130135140ProLysLeuLeuGlySerLeuIleAlaThrGlyPheProTyrAspVal145150155160AlaArgAspArgGluAsnLeuThrTyrLeuGluArgValLeuPheLys165170175GlyIleThrValArgArgProGlyAlaAlaAlaLeuAspLeuAlaTyr180185190ValAlaCysGlyArgLeuAspGlyPheTrpGluValLysLeuAsnPro195200205TrpAspValAlaAlaGlyTrpLeuLeuValGluGluAlaGlyGlyArg210215220IleThrGlyIleHisGlyGluProTyrArgMetGlyAsnArgTyrLeu225230235240MetAlaSerAsnGlyHisIleHisGluGluLeuLeuAlaThrIleHis245250255GlyArg<210>94<211>262<212>PRT<213>Meiothermuschliarophilus<400>94MetAspLeuAspTrpThrLeuValHisProSerGlnGlyValProGlu151015SerAlaTrpArgAlaValGluGluAlaArgAlaAlaGlyValArgLeu202530AlaValAlaThrGlyArgProPheGlyGlyPheGlyLeuGluTyrAla354045LeuArgMetAsnProGluGlyTyrHisAlaPheSerAsnGlySerLeu505560IleAlaArgGlyAlaGluLeuValHisArgValAlaMetProGluAla65707580ThrTyrArgArgMetValLeuHisSerArgGluGlnGlyLeuProPhe859095TyrValSerGlyAlaSerGlyArgLeuTyrSerGluAsnProProArg100105110GluLeuHisArgPheAlaLeuArgMetGlyValGlyTyrGluArgVal115120125AspLeuLeuGluLeuProGluProCysValGlyGlyValPheValIle130135140ThrGlyGlyLeuTrpAspSerLeuArgProSerLeuThrAlaValGlu145150155160GlyLeuAspTrpLeuGluTyrValValGlyGluGlyGluValValAla165170175ValAlaAspProLysGlyValSerLysAlaSerAlaLeuArgTrpMet180185190AlaGluArgTyrGlyLeuAlaMetGluGluValAlaMetIleGlyAsp195200205SerLeuAsnAspLeuGluAlaIleArgGlnValGlyLeuGlyIleAla210215220MetGlyAsnAlaGluProAlaIleLeuGluAlaAlaAspAlaValVal225230235240GlyArgLeuGluGluAspGlyPheAlaGlnAlaValArgLeuCysLeu245250255GluProSerGluValArg260<210>95<211>258<212>PRT<213>Meiothermusruber<400>95MetAspLeuArgAlaTyrLeuGlnThrAlaLeuAspAlaAlaTyrLeu151015AlaLysGlyIleHisGlnTyrTyrGlnGluLysGlyPheThrGlnSer202530ThrLysSerThrProThrAspLeuValThrGlnAlaAspHisGluSer354045GluAlaAlaIleArgGluLeuIleAlaSerArgHisProAspHisVal505560ValLeuGlyGluGluGlnGlyGlnAspLysGluGlyAlaPheArgTrp65707580IleValAspProLeuAspGlyThrValAsnTyrAlaHisGlyPhePro859095PheTyrAlaValSerIleGlyLeuGluAlaHisGlyGluValValLeu100105110GlyValValLeuAspThrAlaArgGlyGluLeuPheThrAlaThrLys115120125GlyGlyGlyAlaTyrLeuAsnGlyArgProIleArgValSerThrArg130135140SerThrLeuValGlySerLeuLeuAlaThrGlyPheProTyrAspVal145150155160SerLysAspThrGluAsnLeuValTyrPheGlnArgAlaLeuThrLys165170175GlyLeuMetValArgArgProGlyAlaAlaAlaLeuAspLeuAlaTyr180185190ValAlaAlaGlyArgLeuAspGlyPheTrpGluValLysLeuAsnPro195200205TrpAspValAlaAlaGlyTrpLeuIleValSerGluAlaGlyGlyArg210215220IleThrGlyLeuGlnGlyGluAspTyrArgLeuGlyAsnArgTyrLeu225230235240ValAlaSerAsnGlyLeuIleHisGlyProLeuLeuAspThrIleHis245250255GlyArg<210>96<211>272<212>PRT<213>Meiothermusruber<400>96MetIleArgLeuValAlaLeuAspLeuAspGlyThrPheTyrAlaGly151015ArgSerLeuGlyValProAlaSerAlaTrpGluAlaValGluLysGly202530ArgArgHisGlyLeuArgPheAlaValCysThrGlyArgProGlnGly354045GlyTyrGlyLeuGluTyrAlaLysArgLeuGluProAsnGlyAlaHis505560ValPheAsnAspGlyAlaSerValCysAspAlaThrGlyArgSerLeu65707580GlnAlaAspProLeuProHisLeuSerGluLeuValGlyLeuAlaArg859095AlaHisAlaLeuProPheAspLeuMetGlyAlaGluGlyGlyArgTyr100105110TyrGluGluGlyLeuMetProProGluLeuLeuSerHisIleGluThr115120125ThrGlyValGluAlaArgSerAlaArgLeuGluGluIleGluGluThr130135140LeuValArgLeuTrpPheValValGlyAspLeuGlyLeuTrpGluSer145150155160ValLysProGluLeuValAlaLeuProSerIleAspLeuAlaGluTyr165170175IleSerProArgGluValIleAlaGlyValIleArgLysGlyValSer180185190LysAlaThrGlyLeuArgTrpLeuAlaGlnTyrTyrGlyLeuSerLeu195200205SerGluIleAlaMetIleGlyAspSerHisAsnAspLeuGluAlaIle210215220ArgGluAlaGlyLeuGlyIleAlaMetGlyAsnAlaValGluAlaIle225230235240ArgAlaAlaAlaArgHisIleThrGlyHisValArgGluAspGlyPhe245250255AlaGluAlaValGluTyrIleLeuAlaTyrAsnArgGlnAsnGlnLeu260265270<210>97<211>278<212>PRT<213>Meiothermusruber<400>97MetLeuGlnLysIleSerArgLeuProIleGlnLeuValLeuIleAsp151015IleAspGlyThrLeuPheGlyProGlnGlyValProGluCysAlaTrp202530GluAlaAlaGlnArgAlaArgAlaAlaGlyLeuHisLeuSerIleCys354045ThrGlyArgProGlyArgGlyPheAlaLeuGluTyrAlaLysArgLeu505560AspProAlaGlyLeuHisIlePheGluSerGlyAlaValValValSer65707580GlyGlnGlyGluValValGlnAlaSerThrLeuProProSerValTyr859095GlnArgLeuLeuAlaLeuSerArgAlaTyrGlyLeuProPheGluVal100105110TyrThrAlaGluGlyGlyPheTyrArgGluSerGlnHisProAspLeu115120125ValPheHisGluSerMetLeuGlyCysProAlaValValCysGlyLeu130135140AspAspValGlyAlaGlnValValArgValGlnPheValTrpArgAla145150155160SerProAlaTrpGlnAlaValArgAlaGlnIleAlaGlnMetThrGlu165170175ValGluLeuHisGluAlaThrSerProGlyMetProGlyValGlyPhe180185190SerSerValThrAlaAlaGlyValSerLysArgAlaAlaAlaGluTrp195200205ValAlaAlaArgLeuAspLeuAspLeuSerArgCysAlaMetValGly210215220AspGlyGluAsnAspLeuGluLeuIleGlnAlaAlaGlyLeuGlyIle225230235240AlaMetGlyAsnAlaProGluArgValLysGlnAlaAlaGlnArgVal245250255ValGlyProValGluAlaCysGlyLeuGluGlnAlaLeuGluIleIle260265270GlnAsnHisAlaArgSer275<210>98<211>216<212>PRT<213>Meiothermusruber<400>98MetLysLeuArgAlaLeuLeuPheAspLeuAspGlyThrLeuAlaAsp151015ThrAspArgLeuHisGluGlnAlaTrpLeuGluGlyLeuSerArgTyr202530GlyIleGlnGlyAspHisHisPheTyrGlnThrGlnIleSerGlyGly354045LeuAsnProGluIleValGlnArgLeuLeuProGlnLeuSerGluAla505560GluGlyGlnArgPheIleGluGlnLysGluAlaArgPheArgGluLeu65707580AlaThrThrValGlnProLeuProGlyLeuArgValLeuTrpAsnTrp859095AlaGlyGluArgGlyLeuGlyArgAlaLeuValSerAsnAlaProArg100105110GlyAsnAlaLeuTyrLeuLeuGluArgLeuGlyLeuValPheAspHis115120125IleValLeuSerGluAspLeuProAlaGlyLysProAspProLeuPro130135140TyrArgMetAlaLeuGlnHisLeuAsnLeuAlaProGlnGluAlaLeu145150155160AlaPheGluAspSerProSerGlyValArgSerAlaValGlyAlaGly165170175LeuProThrValAlaLeuThrThrGlyHisProProGluAlaLeuAla180185190GlnAlaGlyAlaPheLeuCysIleProAspTyrThrAspProArgLeu195200205TrpAspTrpLeuArgAlaLeuGly210215<210>99<211>258<212>PRT<213>Meiothermusruber<400>99MetAspLeuArgProTyrLeuGluAlaAlaLeuAspAlaAlaTyrLeu151015AlaLysGlyIleHisGlnTyrTyrGlnGluLysGlyPheThrHisSer202530ThrLysSerThrProThrAspValValThrGlnAlaAspHisGluAla354045GluAlaAlaIleArgAlaLeuIleAlaGluArgPheProAspHisVal505560ValLeuGlyGluGluGlnGlyGlnGluGlyGluGlyGluPheArgTrp65707580IleValAspProLeuAspGlyThrValAsnTyrAlaHisGlyPhePro859095PheTyrGlyValSerIleGlyLeuGluValArgGlyGluValValLeu100105110GlyValValLeuAspThrAlaArgGlyAspLeuPheThrAlaThrLys115120125GlyGlyGlyAlaTyrLeuAsnGlyArgProLeuArgValSerGlnArg130135140ThrThrLeuValGlySerLeuLeuAlaThrGlyPheProTyrAspVal145150155160AlaArgAspProGluAsnLeuThrTyrPheGlnArgAlaLeuAlaLys165170175GlyLeuThrValArgArgProGlyAlaAlaAlaLeuAspLeuAlaAsn180185190ValAlaAlaGlyArgLeuAspGlyPheTrpGluValLysLeuAsnPro195200205TrpAspValAlaAlaGlyTrpLeuLeuIleThrGluAlaGlyGlyGln210215220ValThrGlyPheGlnGlyGluAlaTyrArgLeuGlyAsnArgTyrLeu225230235240ValAlaSerAsnGlyArgIleHisGlnAlaLeuLeuGluAlaLeuGln245250255GlyArg<210>100<211>258<212>PRT<213>Meiothermussilvanus<400>100MetAspLeuGlnArgTyrLeuGluAlaAlaLeuAspAlaAlaTyrLeu151015AlaArgGlyIleHisLeuTyrTyrArgGluLysGlyPheAlaLeuGlu202530SerLysThrSerProThrAspLeuValThrGlnAlaAspArgGluSer354045GluGluAlaIleArgSerLeuLeuLeuGluArgPheProAspHisVal505560ValLeuGlyGluGluGlyGlyGlnGluGlyHisSerAspPheArgTrp65707580IleValAspProLeuAspGlyThrValAsnTyrAlaHisGlyPhePro859095PheTyrAlaValSerIleAlaLeuGluValGlyGlyGluValValVal100105110GlyAlaValLeuAspThrAlaArgGlyGluLeuPheThrAlaLeuLys115120125GlyGluGlyAlaPheGlnAsnGlyArgProIleArgValSerSerThr130135140AlaThrLeuIleGlySerLeuLeuAlaThrGlyPheProTyrAspVal145150155160AlaLysAspAlaGluAsnLeuThrTyrPheGlnArgAlaLeuAlaLys165170175GlyLeuThrValArgArgProGlyAlaAlaAlaLeuAspLeuAlaTyr180185190ValAlaAlaGlyArgLeuGluGlyPheTrpGluValLysLeuAsnPro195200205TrpAspValAlaAlaGlyTrpLeuLeuIleThrGluAlaGlyGlyThr210215220ValSerGlyIleGlnGlyGluProTyrArgLeuGlyAsnArgTyrLeu225230235240ValAlaSerAsnGlyLysIleHisGluGlnLeuLeuAspThrLeuHis245250255GlyArg<210>101<211>213<212>PRT<213>Meiothermussilvanus<400>101MetArgAlaLeuLeuPheAspLeuAspGlyThrLeuAlaAsnThrAsp151015ArgLeuHisGluGlnAlaTrpLeuGluThrLeuArgPheTyrGlyIle202530GluGlyAspHisHisPheTyrGlnThrGlnIleSerGlyGlyLeuAsn354045ProGluIleValArgArgLeuLeuProGlnLeuSerGluAlaGluGly505560GluAlaPheIleAlaArgLysGluArgArgPheArgGluLeuAlaGln65707580AspLeuArgAlaLeuProGlyLeuAspAlaLeuLeuAlaTrpAlaArg859095ArgLysLysLeuLeuThrGlyLeuValThrAsnAlaProHisGluAsn100105110AlaArgHisValThrGlnAlaLeuGlyLeuSerPheAspValValVal115120125LeuAlaGluGluLeuAlaAlaGlyLysProAspProLeuProTyrArg130135140ValAlaLeuGluArgLeuAspLeuGlyAlaGlnGluAlaLeuAlaPhe145150155160GluAspSerProAlaGlyValLysAlaAlaValGlyAlaGlyIlePro165170175ThrIleGlyLeuThrThrGlyHisProProGluAlaLeuLysAlaAla180185190GlyAlaPheLeuLeuIleAlaAspPheThrAspProGlnLeuTrpLys195200205TyrLeuGluArgSer210<210>102<211>258<212>PRT<213>Meiothermustaiwanensis<400>102MetAspLeuArgAlaTyrLeuGlnThrAlaLeuAspAlaAlaTyrLeu151015AlaLysGlyIleHisGlnTyrTyrGlnGluLysGlyPheThrGlnSer202530SerLysSerThrProThrAspLeuValThrGlnAlaAspHisGluSer354045GluAlaAlaIleArgGluLeuIleAlaAlaArgHisProAspHisVal505560ValLeuGlyGluGluGlnGlyGlnAspLysGluGlyAlaPheArgTrp65707580IleValAspProLeuAspGlyThrValAsnTyrAlaHisGlyPhePro859095PheTyrAlaValSerIleGlyLeuGluAlaHisGlyGluValValLeu100105110GlyValValLeuAspThrAlaArgGlyGluLeuPheThrAlaThrLys115120125GlyGlyGlyAlaTyrLeuAsnGlyArgProIleArgValSerThrArg130135140SerThrLeuValGlySerLeuLeuAlaThrGlyPheProTyrAspVal145150155160SerLysAspThrGluAsnLeuThrTyrPheGlnArgAlaLeuThrLys165170175GlyLeuMetValArgArgProGlyAlaAlaAlaLeuAspLeuAlaTyr180185190ValAlaAlaGlyArgLeuAspGlyPheTrpGluValLysLeuAsnPro195200205TrpAspValAlaAlaGlyTrpLeuIleValSerGluAlaGlyGlyArg210215220ValSerGlyLeuGlnGlyGluAspTyrArgLeuGlyAsnArgTyrLeu225230235240ValAlaSerAsnGlyLeuIleHisGlnProLeuLeuAspThrLeuHis245250255GlyArg<210>103<211>216<212>PRT<213>Meiothermustaiwanensis<400>103MetLysLeuArgAlaLeuLeuPheAspLeuAspGlyThrLeuAlaAsp151015ThrAspArgLeuHisGluGlnAlaTrpLeuGluGlyLeuSerArgTyr202530GlyIleGlnGlyAspHisArgPheTyrGlnAlaGlnIleSerGlyGly354045LeuAsnProGluIleValAlaArgLeuLeuProGlnLeuSerProAsp505560GluGlnValAlaPheIleGluGlnLysGluAlaArgPheArgGluLeu65707580AlaSerGluValGlnProLeuProGlyLeuArgValLeuTrpAspTrp859095AlaGlnSerGlnGlyLeuArgArgAlaLeuValSerAsnAlaProArg100105110GluAsnAlaHisTyrLeuLeuGluArgLeuGlyLeuMetPheAspAla115120125IleValLeuSerGluAspLeuProAlaGlyLysProAspProLeuPro130135140TyrArgThrAlaLeuGlnHisLeuAsnIleGlyProGlnGluAlaLeu145150155160AlaPheGluAspSerProSerGlyValArgSerAlaValGlyAlaGly165170175LeuArgThrValAlaLeuThrThrGlyHisProProHisAlaLeuGlu180185190GlnAlaGlyAlaPheLeuCysIleProAspPheThrAspProArgLeu195200205TrpAspTrpLeuGlnLysMetGly210215<210>104<211>271<212>PRT<213>Meiothermustimidus<400>104MetIleGlyLeuValLeuIleAspValAspGlyThrLeuTyrGlyPro151015AspGlyValProGluCysAlaTrpGluAlaThrGlnLysAlaArgAla202530AlaGlyIleHisLeuAlaLeuCysThrGlyArgProGlyArgGlyPhe354045AlaLeuGluTyrAlaArgArgIleAspProGluGlyLeuHisIlePhe505560GluSerGlyAlaValValValAlaGlyAsnGlyGlnValIleLysSer65707580SerProLeuSerProGluAlaTyrArgGluLeuLeuAlaLeuSerArg859095GluTyrAlaIleProPheGluValTyrThrAlaAspGlyGlyPhePhe100105110IleGluGlnAsnHisProAspLeuGluAlaHisGlnArgMetLeuGly115120125PheMetAlaLysValAlaProLeuAspGlnProProGlyGluValIle130135140ArgValGlnTyrValThrProLeuGluGlyGlnTrpGlnGluValArg145150155160GlyArgValGlnArgIleGluGlyAlaGluLeuHisGluAlaThrSer165170175ProGlyValProGlyIleGlyPheAsnSerValThrAlaAlaGlyVal180185190SerLysLeuSerAlaAlaArgTrpValAlaGluArgTyrGlyLeuGly195200205LeuGluHisCysAlaMetValGlyAspGlyGluAsnAspLeuGluLeu210215220IleLeuAlaAlaGlyValGlyIleAlaMetGlyAsnAlaProGlnSer225230235240ValLysGluArgSerLysArgValValGlyArgValGluGluCysGly245250255LeuAlaGlnAlaLeuGluTrpValAlaAlaAlaGluGlyArgSer260265270<210>105<211>214<212>PRT<213>Meiothermustimidus<400>105MetArgProThrThrLeuLeuPheAspLeuAspGlyThrLeuLeuAsp151015ThrGluProGlyIleLeuAlaCysLeuArgHisThrLeuGluHisPhe202530GlyLeuProThrGlnGlyAspAlaGluLeuArgArgPheIleGlyPro354045ProLeuGluGlnAlaTrpArgGluLeuValGlyAlaAlaHisValGlu505560GluAlaValGluIleTyrArgArgCysTyrAspLeuLysGlyLysPhe65707580LysAlaAlaValPheGluGlyIleGluAlaAlaLeuHisGluLeuAla859095TyrHisHisThrLeuIleValAlaThrSerLysArgGlnValPheAla100105110GluGluMetLeuGluArgPheGlyLeuSerSerTyrPheLysAlaVal115120125TyrGlyValThrProProGlnLeuSerGluProLysAlaHisLeuIle130135140GlyArgIleLeuArgAspHisGlyLeuSerProGlnGlnAlaValMet145150155160ValGlyAspArgGluPheAspValIleGlyAlaLysAlaAsnGlyMet165170175AlaCysValGlyValLeuTrpGlyTyrGlySerArgGluGluLeuAla180185190PheHisGlyAlaSerLeuLeuCysGluSerProArgGluLeuValGlu195200205AlaValAlaGlyLeuAla210<210>106<211>223<212>PRT<213>Meiothermustimidus<400>106MetLysArgAlaValLeuPheAspValGlyAspThrLeuIleLeuGly151015HisProLysLeuTrpLeuTrpProLeuLeuGlnGluArgGlyLeuAla202530GluLysAlaAspGlySerArgLeuMetGluAlaValMetAlaAlaTyr354045ArgValTyrAsnGluArgHisMetGlnAlaThrThrIleGluAlaAla505560LeuProValTrpAlaGluPheHisArgThrLeuLeuSerGlyIleGly65707580LeuGlyGluHisAlaGlyGluIleSerGlyPheLeuCysGluAsnTrp859095ArgAsnProLysValTrpProLeuThrProGlyAlaLysGluValLeu100105110GlyGluLeuLysGlyArgGlyTyrLysLeuGlyValValSerAsnTrp115120125AspAlaLeuLeuProGlyValLeuGluAlaThrGlyLeuAlaProPhe130135140PheAspPheValAlaAlaSerAlaLeuValGlyAlaAlaLysProAsp145150155160ProArgIlePheArgValAlaLeuGluGlyLeuGlyValGluProHis165170175GlnAlaLeuHisValGlyAspSerProAspAspLeuGlyGlyAlaAla180185190AlaAlaGlyValGluAlaLeuLeuPheAspProTyrArgArgAsnPro195200205GluAlaLeuHisGluLeuSerGlyValLeuGluArgLeuGluAla210215220<210>107<211>198<212>PRT<213>Meiothermustimidus<400>107MetSerLysArgIleArgAlaIleCysPheAspPheAspGlyThrLeu151015AlaGlnPheGluGlyAspPheAlaArgLeuThrAlaLeuCysCysAsp202530GlnLeuGlyIleAspProGlyAspArgAlaPheLeuAlaThrTyrGlu354045ArgGluIleArgAlaGluGlyHisValThrPheAlaLeuAlaLeuArg505560ArgAlaLeuGluThrHisGlyTyrProValProAlaGlyLeuGluAla65707580PheAlaAlaGluValThrAlaArgTyrAlaAlaGluValArgLeuLeu859095ProGlyValArgGluLeuLeuAlaTyrPheAlaProLeuProLysAla100105110IleIleThrAsnGlyProAlaAspMetGlnTrpAlaAlaIleArgSer115120125ValGlyLeuGluArgGluPheGluAlaIleValValSerGlyAspPro130135140AspValAlaValArgLysProHisProArgIlePheHisLeuAlaCys145150155160GluArgLeuGlyValProProHisGluThrLeuMetIleGlyAspHis165170175LeuGluAlaAspIleGlnGlyAlaIleAlaAlaGlyLeuGlnGlyLeu180185190HisArgProAsnSerAla195<210>108<211>237<212>PRT<213>Meiothermustimidus<400>108MetIleProGluGluLeuAlaLeuLeuAlaArgGlnProLeuLeuVal151015IleCysAspTyrAspGlyThrLeuAlaProIleAlaIleHisProSer202530GlnAlaPheProGlnArgGlyAlaArgGluAlaLeuArgAlaLeuGln354045HisHisProLysHisGlnIleMetValLeuThrGlyArgArgAlaGln505560GluValLeuAspPheLeuGluLeuProGlyLeuArgValIleGlyLeu65707580HisGlyLeuGluTrpProGlyArgProGluProProArgAspArgGlu859095AlaIleGlnArgIleValSerArgLeuProProLeuGluGlyLeuTrp100105110LeuGluAspLysGlyTrpThrLeuAlaValHisTyrArgAspAlaPro115120125GluSerGlnGlnSerLysAlaAlaGluLeuLeuAlaAlaValProLeu130135140ProGluGlyTrpGluAlaMetProGlyLysLysValCysGluTyrArg145150155160ProIleGlyTyrGlyLysGlyTrpAlaValGluGlnLeuMetLeuAsp165170175HisProSerHisHisProValPheIleGlyAspAspArgThrAspGlu180185190GluGlyPheAlaAlaValLeuArgLeuGlyGlyThrAlaValLysVal195200205GlyGlyGlyGluSerLeuAlaProTyrArgLeuGlyAsnSerLeuGlu210215220ValValGluLeuLeuThrArgTrpAlaArgGlyTyrAsp225230235<210>109<211>249<212>PRT<213>Meiothermustimidus<400>109MetArgAlaLeuIlePheAspValAspGlyValIleAlaGluThrGlu151015GluGlyHisArgLeuAlaPheAsnArgAlaPheAlaGluAlaGlyLeu202530AspIleGluTrpSerGlnGluLeuTyrGluArgLeuLeuTrpValThr354045GlyGlyLysGluArgIleAlaHisTyrLeuTyrHisCysProGluCys505560ProLysLeuLeuAspAlaAspIleAlaArgLeuHisTrpArgLysThr65707580GluLeuTyrAsnGlnIleValAlaAlaGlyGlyValProPheArgPro859095GlyValLeuArgLeuTrpArgGluAlaArgAlaGlnGlyValLysLeu100105110AlaIleAlaThrThrThrSerLeuProAsnValGluValLeuLeuArg115120125GlnAlaGlyGluGluValLeuGlyTrpPheGluThrIleValAlaGly130135140AspMetValProArgLysLysProAlaProAspValTyrLeuLysVal145150155160LeuGluAsnLeuGlyLeuAlaProGluGluAlaLeuAlaIleGluAsp165170175SerGlnAsnGlyLeuIleAlaAlaGlnLysAlaGlyIleProThrLeu180185190IleThrTyrSerHisTyrThrArgGluGlnArgPheGluGlyAlaLeu195200205AlaValLeuGluHisLeuGlyGluProGluLeuProAlaArgValVal210215220AlaGlyProArgSerCysAlaMetValValSerLeuGluValLeuArg225230235240GluTrpTyrGlyArgPheValArgAla245<210>110<211>157<212>PRT<213>Meiothermustimidus<400>110MetLeuArgProLysAlaGlnLeuSerSerValLeuGluIleThrPro151015GlnTrpLeuLysThrArgGlyIleLysGlyLeuLeuLeuAspLeuAsp202530AsnThrLeuValProTyrLysPheLysGlyGluProProGluGluLeu354045ValAlaTrpValLysSerLeuGluAlaAlaGlyIleArgValPheLeu505560ValSerAsnAlaGlnHisLysArgLeuArgAlaTrpSerAlaLysLeu65707580GlyValGluGlyIleGlyLeuAlaGlyLysProTrpPheGlyIleArg859095LysGlyLeuLysArgLeuGlyLeuSerProSerGlnValAlaMetVal100105110GlyAspGlnValPheThrAspValLeuGlyGlyAsnLeuAlaGlyVal115120125TyrThrIleLeuValThrProIleSerGlnLysGluLeuGlyTyrThr130135140LysLeuIleArgArgLeuGluArgLeuIleLeuLysLeu145150155<210>111<211>221<212>PRT<213>Meiothermustimidus<400>111MetLysAlaLeuValPheAspPheAspGlyThrIleLeuAspThrGlu151015GlnAlaGluTyrThrAlaTrpThrGlnAlaTyrAlaArgTyrGlyGly202530GluLeuThrLeuHisAspTyrLeuProLeuIleGlyThrAlaGluPro354045ValPheAspLeuTyrAlaHisLeuGluAlaGlnIleGlyProTyrAsp505560ArgGluAlaPheAspArgGluArgGlnGluValLeuHisSerLeuLeu65707580AlaGlnLeuGluProLeuProGlyValProGluCysLeuGluGlnAla859095ArgLysLeuGlyLeuLysLeuAlaValAlaSerSerSerSerAlaAla100105110TrpValLeuGlyHisLeuGluGlnHisHisLeuLeuGlnAlaPheAsp115120125HisValIleThrArgGluTyrValAlaArgThrLysProAspProAla130135140LeuPheLeuLysAlaAlaGluAlaLeuGlnValAlaProAlaGluCys145150155160ValAlaValGluAspSerLeuAsnGlyValArgAlaAlaArgAlaAla165170175GlyMetPheThrIleAlaValProAsnProLeuThrArgHisGlnAsp180185190LeuArgGlnAlaAspValLeuLeuProSerLeuAlaGlyLeuGluLeu195200205GluProPheLeuSerSerLeuLysProValHisProArg210215220<210>112<211>274<212>PRT<213>Meiothermustimidus<400>112MetGlyGlyArgCysArgGlyProValValIleArgLeuIleGlyVal151015AspLeuAspTrpThrPheValHisProSerGlnGlyValProAlaSer202530AlaTrpGlnAlaValGluGluAlaLysAlaAlaGlyMetArgPheAla354045ValValThrGlyArgProPheGlyGlyTyrGlyLeuGluTyrAlaLeu505560LysMetGluProGlnGlyTyrHisAlaPheSerAsnGlySerLeuIle65707580ThrGlnGlyAlaThrLeuValHisSerValAlaMetSerProSerThr859095TyrArgArgMetValLeuHisSerArgAlaGlnGlyLeuProPheTyr100105110ValSerGlyAlaSerGlyArgLeuTyrSerGluAsnProProArgGlu115120125LeuHisArgPheAlaGluArgMetGlyValSerTyrGlnArgIleAsp130135140LeuLeuGluLeuProGluProCysValGlyGlyValPheValLeuAsp145150155160GlyGlnLeuTrpAsnAspLeuArgGlnAlaIleThrAlaValGluGly165170175LeuAspTrpLeuGluTyrLeuValGlyGluGlyGluValValAlaVal180185190AlaAspProLysGlyThrSerLysAlaSerAlaLeuArgTrpMetAla195200205GluArgTyrGlyLeuAlaMetGluGluValAlaMetIleGlyAspSer210215220LeuAsnAspLeuGluAlaIleLysGlnValGlyLeuGlyIleAlaMet225230235240GlyAsnAlaGluGluValValArgGluAlaAlaSerValValValAla245250255GlyLeuGluGluAspGlyPheAlaGlnAlaValArgLeuCysLeuAla260265270ArgArg<210>113<211>216<212>PRT<213>Meiothermustimidus<400>113MetArgAlaLeuLeuPheAspLeuAspGlyThrLeuAlaAspThrAsp151015ArgLeuHisGluGlnAlaTrpLeuGluValLeuLeuProTyrGlyIle202530ArgGlyAspHisAlaPheTyrGlnGlnHisIleSerGlyHisLeuAsn354045ProGluIleValSerArgLeuLeuProHisLeuProProLeuGluArg505560ThrAlaLeuIleGluValLysGluArgArgPheArgGluLeuAlaGln65707580GlyLeuLysAlaLeuProGlyLeuGluGlyLeuTrpArgTrpAlaArg859095GluArgGlyLeuThrLeuAlaLeuValThrAsnAlaProArgProAsn100105110AlaGluHisValLeuGlnAlaLeuGlyAlaGluPheAspLeuValVal115120125LeuAlaGluGluLeuAlaAlaGlyLysProAspProLeuProTyrArg130135140ThrAlaLeuGlyArgLeuGlyLeuAspProAlaGluAlaLeuAlaPhe145150155160GluAspSerProSerGlyValArgSerAlaValGlyAlaGlyIleArg165170175ThrIleGlyLeuThrThrGlyHisAspProArgGlyLeuLeuGluAla180185190GlyAlaPheLeuLeuIleAspAspPheSerAspGlyArgLeuTrpGlu195200205TyrLeuGluGlyGluSerAspSer210215<210>114<211>223<212>PRT<213>Meiothermusrufus<400>114MetLysLeuLysAlaLeuLeuPheAspLeuAspGlyThrLeuAlaAsp151015ThrAspArgLeuHisGluGlnAlaTrpLeuGluGlyLeuAlaArgTyr202530GlyIleGlnAlaAspHisHisTyrTyrGlnThrGlnIleSerGlyGly354045LeuAsnProGluIleValAlaArgLeuLeuProGlnLeuSerProAla505560GluAlaAlaAlaPheIleGluGlnLysGluAlaArgPheArgGluLeu65707580AlaAlaGlyValThrProLeuProGlyLeuGlnGluLeuTrpAlaTrp859095AlaAsnGlyHisArgLeuArgLeuAlaLeuValSerAsnAlaProArg100105110GlnAsnAlaGluHisMetLeuLysArgLeuGlyLeuHisPheAspCys115120125ThrValLeuSerGluGluLeuProAlaGlyLysProAspProLeuPro130135140TyrArgThrAlaLeuAlaGlnLeuGlyLeuGlyProGluGluAlaLeu145150155160AlaPheGluAspSerProSerGlyValArgSerAlaValGlyAlaGly165170175LeuArgThrValAlaLeuThrThrGlyHisProAlaHisArgLeuGlu180185190GluAlaGlyAlaPheLeuAlaValAlaAspPheThrAspGluArgLeu195200205TrpAlaTrpLeuGluGlnGlnSerProProSerThrProArgGlu210215220<210>115<211>218<212>PRT<213>Mesotogainfera<400>115MetIleLysAlaAlaIlePheAspMetAspGlyValIleIleAspSer151015GluLysIleTyrArgArgAlaCysThrGluLeuValAspGluLeuGly202530GlyLysIleSerValGluLeuPheGluArgGlnMetGlyLeuLysMet354045SerGluThrGlnLysValValValGlnThrAlaGlyLeuGluIleGlu505560ProGluGluPheGlyLysArgTyrMetGluArgTyrLeuGluLeuAla65707580ArgGluThrLeuValProAsnProGlyLeuValAsnLeuLeuAspPhe859095LeuSerGluLysValGluLeuAlaIleAlaSerSerThrGluLysAla100105110AlaValGluGluLeuMetLysArgIleGlyValLeuAspTyrPheGlu115120125IleIleValGlyGlyAspGluValAspGluSerLysProSerProMet130135140IleTyrLeuLysAlaSerGluLeuLeuGlyValAsnProGluGluCys145150155160IleValIleGluAspSerProAsnGlyIleLysSerGlyIleGlyAla165170175GlyMetGluValLeuGlyValArgHisGlyGluAsnAlaHisLeuAsp180185190LeuSerAlaSerSerHisValPheAspAspLeuTyrGlyValLysGlu195200205TyrLeuGluThrValLeuAsnGlyAsnAla210215<210>116<211>222<212>PRT<213>Metallosphaerasedula<400>116MetTrpIleValAlaSerAspPheAspArgThrLeuSerHisGluAsp151015AspSerPheValMetArgLysGluValAlaSerLysIleAsnHisPhe202530SerThrIleHisArgPhePheValValThrGlyArgGluGluArgTyr354045MetArgValLeuAlaProAspLeuArgProThrGlyTrpValLeuGlu505560AsnGlyAlaLeuLeuIleLeuGlyAspArgArgIleLeuAsnValPro65707580GluAspTrpPheGluThrArgLysIleIleGlyGluLysLeuThrLys859095PheGlyIleSerTyrSerLeuGlyAspValIleIleTyrValAsnSer100105110TrpAsnGlyLysLeuAspLeuGlyProGluValArgIleGluArgAsn115120125ArgGluAspAlaMetIleLeuProGlyAsnValAspLysGlyThrGly130135140LeuArgArgAlaIleGlnGluMetHisLeuGluGlyLysIleValAla145150155160ValGlyAspAlaGluAsnAspGluSerLeuPheLysValAlaAspVal165170175LysValAlaValAlaAsnAlaIleProProIleLysArgMetAlaAsp180185190LeuValMetGluLysGluAspGlyGluGlyValValGluLeuLeuAsp195200205MetIleLeuSerGlyArgPheProLysAsnValAspValAsn210215220<210>117<211>224<212>PRT<213>Methanocellaconradii<400>117MetIleLysAlaValValAlaAspIleAspGlyThrLeuThrAspMet151015GlnArgArgIleSerValGluAlaIleValAlaIleArgLysLeuPro202530IleProValValLeuAlaSerGlyAsnValIleCysPheMetArgAla354045AlaSerLysLeuIleGlyAlaSerGluAlaMetIleGlyGluAsnGly505560GlyValValGlnValGlyTyrAspSerProProValValPheAlaAsp65707580IleGluGluCysArgArgAlaAlaAlaLeuLeuGlnArgArgPhePro859095GlyLeuGluGlnLeuAspAlaArgTyrArgLeuSerGluLeuAlaPhe100105110ArgArgThrValAspAlaAsnGluLeuArgAlaAlaLeuAspMetGlu115120125TyrProSerLeuGluValValAspThrGlnPheAlaLeuHisLeuLys130135140HisLysSerValAsnLysGlyThrGlyLeuValAsnValAlaSerMet145150155160MetGlyPheLysProGluGluPheAlaAlaLeuGlyAspSerAlaAsn165170175AspLeuProMetPheGluAlaAlaGlyLeuGlyLeuAlaValGlyAsn180185190AlaValProGluLeuLysAspValAlaAspTyrValAlaValLysPro195200205TyrGlyGluGlyAlaAlaGluAlaLeuArgTrpLeuAlaArgGlnLeu210215220<210>118<211>237<212>PRT<213>Methanocellaconradii<400>118MetAlaGluArgCysSerValLysIlePheLeuTyrArgAlaValLeu151015PheAspMetAspGlyValIleAlaAspThrMetProLeuHisTyrGlu202530AlaTrpArgArgAlaPheGluAlaPheGlyValHisValAspLysMet354045AspValTyrLeuArgGluGlyMetThrThrMetGluMetGlyLysAsp505560IleAlaArgSerLysGlyMetGluLeuSerGluGlyGluLeuAsnSer65707580IleValGluLeuLysThrArgIlePheAsnGluLeuValAspSerSer859095ValArgLeuTyrGluGlyValProGluThrLeuThrMetLeuArgAsn100105110AsnGlyMetLysLeuAlaLeuValThrGlySerArgArgThrSerAla115120125MetAlaValLeuLysLysValGlyLeuGluGlyAlaPheAspAlaIle130135140ValAlaAlaGluAspValArgArgGlyLysProAspAlaGluProTyr145150155160LeuValAlaMetArgAlaValAspValProAlaLeuAsnCysValVal165170175ValGluAsnAlaProLeuGlyIleArgAlaAlaArgAlaAlaLysVal180185190GlyTyrIleIleAlaIleAlaThrThrLeuAspGluAlaHisLeuLys195200205GluAlaAspGluValAlaProSerPheProGluLeuGluGlnCysIle210215220AlaArgArgPheGluAlaArgProGlyArgAlaIleMet225230235<210>119<211>209<212>PRT<213>Methanocellaconradii<400>119MetProArgIleArgGlyLeuIlePheAspLeuAspGlyThrLeuIle151015AspAsnTyrAspGlyTyrMetGluLeuMetLeuArgArgValGlyArg202530AspLeuGlyArgGluPheThrLeuSerHisAlaLysGluLeuTrpTyr354045SerIleAsnSerGluSerArgAspGluValIleMetArgTrpGlyVal505560AsnProAspArgPheTrpThrValPheAsnArgTyrGluAspIleAsn65707580GluLysLeuGlu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lHisSerLeuSerAspIle225230235240GluLysSerAspIleLysProAspLeuValLeuProSerIleLysGlu245250255LeuLeuGluTyrLeuLysIleLeuHisAspGluAspLysGluGlyThr260265270Lys<210>137<211>351<212>PRT<213>Pyrococcusfuriosus<400>137MetSerLysProLeuPheValThrProProTyrValSerThrLeuLeu151015ThrLeuIleGluTyrCysThrTrpLeuIleSerCysProSerLeuLys202530PheProProProCysPheIleMetProThrPheTrpAlaGlyTyrPhe354045CysCysTrpLysSerArgAsnPheLeuPheAsnTyrAsnTyrSerPhe505560IleArgProMetLeuAspPhePheCysSerSerIleCysPheGlyVal65707580LeuArgIleTyrArgPheLeuIleThrThrPheSerThrAspLysAsp859095SerSerProProValGlyTyrHisAsnThrProThrPheGlyAlaGlu100105110PheHisHisLeuSerHisProHisThrSerGlnGlyGlnSerLeuIle115120125ValGlnAsnLysLysPheThrLeuHisHisSerLeuGluGlyArgCys130135140MetLysIleSerLysIleIleGluGlnIleLysSerGluLeuAspLys145150155160LysAspLysLeuArgGluGluAlaLeuGluIleThrArgAspIleIle165170175ArgLeuSerGlyAspAlaIleLysAlaMetHisArgGlyGluLeuGlu180185190LeuAlaHisGluArgLeuGluLysAlaArgGlyLeuValLysGluLeu195200205LysGluLysLeuArgGluHisProAspLeuTyrTyrThrGlyTyrVal210215220GlnAsnAlaAsnGlnGluPheValGluAlaValLeuMetTyrHisTyr225230235240LeuThrAspArgGluPheProSerHisValAspLeuGlyValProSer245250255GlnAspTyrIleLeuGlyValGlyAspPheIleGlyGluLeuArgArg260265270TyrPheLeuIleAsnLeuMetLysGlyAsnLeuAspGluAlaGluSer275280285ThrTyrArgPheMetGluGluValTyrGluGluLeuMetThrLeuGlu290295300TyrProLysGlyLeuValAsnIleArgGlnLysGlnAspGlnAlaArg305310315320TyrThrLeuGluArgThrLeuGluAspLeuThrArgAlaLysValAsn325330335ArgArgValGluGluLysLeuGluAlaPheLeuAsnAspArgSer340345350<210>138<211>207<212>PRT<213>Pyrococcusfuriosus<400>138MetLysIleSerLysIleIleGluGlnIleLysSerGluLeuAspLys151015LysAspLysLeuArgGluGluAlaLeuGluIleThrArgAspIleIle202530ArgLeuSerGlyAspAlaIleLysAlaMetHisArgGlyGluLeuGlu354045LeuAlaHisGluArgLeuGluLysAlaArgGlyLeuValLysGluLeu505560LysGluLysLeuArgGluHisProAspLeuTyrTyrThrGlyTyrVal65707580GlnAsnAlaAsnGlnGluPheValGluAlaValLeuMetTyrHisTyr859095LeuThrAspArgGluPheProSerHisValAspLeuGlyValProSer100105110GlnAspTyrIleLeuGlyValGlyAspPheIleGlyGluLeuArgArg115120125TyrPheLeuIleAsnLeuMetLysGlyAsnLeuAspGluAlaGluSer130135140ThrTyrArgPheMetGluGluValTyrGluGluLeuMetThrLeuGlu145150155160TyrProLysGlyLeuValAsnIleArgGlnLysGlnAspGlnAlaArg165170175TyrThrLeuGluArgThrLeuGluAspLeuThrArgAlaLysValAsn180185190ArgArgValGluGluLysLeuGluAlaPheLeuAsnAspArgSer195200205<210>139<211>204<212>PRT<213>Pyrococcusfuriosus<400>139MetLysGlyLeuIleAlaPheAspLeuGluGlyThrLeuThrAspMet151015IleSerTrpGluIleLeuHisGluLysPheGlyThrCysAspLysAla202530ArgValHisThrSerLeuPheLeuSerGlyLysIleThrTyrHisGlu354045TrpAlaGluMetAspValArgLeuTrpLysGlyArgArgArgGluGlu505560ValGluGluAlaPheSerGlnValThrLeuLysProTyrAlaArgGlu65707580LeuPheGluTrpLeuLysLysAsnAsnPheLysThrAlaIleIleSer859095GlyGlyLeuMetCysLeuAlaArgLysValGlyGluLysLeuGlyVal100105110AspPheIleValAlaAsnGluLeuLysPheAspSerGlnGlyArgIle115120125GluGlyValIleValArgValThrPheAspAsnLysGlyGluIleLeu130135140ArgGlnLeuLysGlnLysValAsnProAsnValThrIleAlaValGly145150155160AspTrpLysAsnAspLysSerMetPheGluGluAlaAspIleSerIle165170175SerLeuGlyAspIleAspGlyAspTyrLysAlaArgAspLeuArgAsp180185190ValLeuGluIleLeuLysSerIleValArgAsnIle195200<210>140<211>235<212>PRT<213>Pyrococcusfuriosus<400>140MetAlaIleThrLeuLysLeuLeuGlnMetCysSerSerLeuMetIle151015IleAlaPheAspPheAspGlyThrLeuValAspSerTyrSerCysIle202530GluGluAlaPheTyrArgAlaLeuLysArgThrTyrProTrpLeuPro354045GlyLysArgTyrIleAlaLysLeuLeuThrLysLeuGluLeuGlnPhe505560GluArgProLysPheGlyLysHisGlyArgLysIleLysProProMet65707580LysIlePheGlnGlyLysPheAlaArgAlaTrpPheGluGluArgAla859095LysLeuThrLysProLeuAspGlyAlaArgGluValLeuGluArgLeu100105110LysGluAspGlyHisIleValIleSerPheSerAlaGluAspPheIle115120125ProGlyIleLysGluTyrArgLeuLysIleSerGlyLeuTyrAspLeu130135140PheAspAspValIleIlePheGlyArgAspIleThrIleCysGluAla145150155160PheSerIleValArgGluLysTyrGlyTyrAspThrPheValTrpVal165170175AspAspLysProTrpArgPheIleGlyArgGlyAspGluAsnThrGlu180185190TyrValTrpMetTyrPheProTyrThrAlaArgPheValSerAspAsp195200205IleLeuAlaGlnIleProHisLeuHisValIleTyrAspLeuTrpSer210215220LeuLeuAspValValLysAsnLeuLysSerArg225230235<210>141<211>211<212>PRT<213>Pyrococcusfuriosus<400>141MetLeuIleIleLeuAspLeuAspAspThrLeuCysAsnThrTrpGlu151015AlaIleArgIleAlaAlaIleArgLeuIleProThrLeuLeuArgLeu202530ArgLysPheArgMetPheAlaTyrIleLeuThrLysArgTyrArgGlu354045LeuGluGluIleArgGluLeuHisIleLeuGlyPheGluGluIleLeu505560AspArgIleIleAsnLysIleTyrLysAsnLeuGluAsnGluGluVal65707580GlnGluIleAlaAsnLeuPheAspArgThrPhePheAlaAsnLeuArg859095LeuTyrProAspValIleProPheLeuGluLysValArgGluMetGly100105110AlaLysIleValLeuValThrAspSerSerSerSerTrpGlnArgArg115120125LysLeuGluValLeuGlyIleLysAspTyrPheAspAlaIleIleVal130135140SerGlyAspThrGlyHisSerLysPheGluProTyrAsnPheHisLeu145150155160AlaArgLysLysPheProArgGluLysLysValTyrValIleGlyAsp165170175ArgAspAspThrAspMetLysGlyGlyLysAlaIleHisAlaThrThr180185190IleLeuValLysArgAlaThrLeuLysGluGlyGlyGlnSerMetGln195200205IleMetLeu210<210>142<211>238<212>PRT<213>Pyrococcusfuriosus<400>142MetTrpIleValPheAspValAspGlyValLeuIleAspThrSerGlu151015SerTyrAspMetAlaThrLysLeuThrValGluTyrPheLeuSerHis202530LeuGlyIleLysLysGluValSerLeuGluIleIleArgAspMetArg354045LysLysGlyValPheSerAspAspPheGluLeuSerGluAlaLeuIle505560LeuMetTyrLeuSerGlyGluAspLeuProLysGlyGluGlyValGlu65707580TyrLeuArgGlnLysLeuGlyIleValLeuAspArgLysAspIleGlu859095ArgValPheAsnThrPheTyrLeuGlyGluIleTyrProAsnSerIle100105110PheLysPheGluGlyLeuTrpArgLysGluLysArgIleValAspIle115120125GluLeuLeuLysLysAlaLysGluThrTyrLysLeuGlyIleValThr130135140GlyArgAspSerLeuGluMetLysLeuAlaGluGluIleIleArgPhe145150155160LysPheAspLysValValThrArgAspMetPheGluLysProAspPro165170175ArgAlaLeuTyrGluValThrGlnGlyGluGluAlaIleTyrIleGly180185190AspSerLysValAspGluGluLeuValGluArgTyrArgArgThrPhe195200205LysGlyAsnValThrTyrLeuMetValGlyArgAspValAsnAspVal210215220AsnGluAlaLeuLysLysIleLeuPheGlnSerTyrIleArg225230235<210>143<211>219<212>PRT<213>Pyrococcusfuriosus<400>143MetValArgValIlePhePheAspIleAspGlyThrLeuLeuThrGlu151015TrpProLeuValLysLeuMetLeuProGlnValTyrGluMetLeuAla202530LysLysLeuGlyValSerLysLysGluAlaArgGluIlePheLeuGly354045GluIleGluLysArgLysGlyThrTyrGluTrpTyrAspTrpAsnPhe505560PhePheSerTyrPheAsnLeuProLeuArgTyrGluAspPheIleArg65707580LysTyrProGluLysIleGluLeuTyrProGlyValArgGluValLeu859095LysGluLeuSerGlyLysTyrLysLeuGlyIleIleThrSerGlyPro100105110HisTyrGlnLeuLeuLysLeuLysValThrAspIleAspLysPhePhe115120125AspValIleIleThrArgAspAspValLysAlaValLysProSerPro130135140LysIlePheLeuAlaGlyLeuGluArgValArgAlaLysProThrGlu145150155160SerLeuMetValGlyAspSerLeuGluAsnAspIleLeuGlyAlaLys165170175AlaLeuGlyPheLysThrValTrpIleAsnArgGlyArgGluLysGly180185190PheAsnLeuProAspPheGluIleTyrGluMetLysGluLeuIleArg195200205ValValGluValAlaGluAsnGluLysAspIle210215<210>144<211>230<212>PRT<213>Pyrococcusfuriosus<400>144MetSerPheLeuAlaIleTyrLysThrAspValLysLeuLeuArgArg151015AspProMetLeuLeuTyrSerValIleMetValPheValLeuLeuLeu202530IleValArgTyrPheLysAspLysValGlyIleTyrTyrTyrProLeu354045AlaLeuLeuThrMetLeuPheIleProMetIlePheGlyMetLeuPro505560GlyPheValMetAlaAspGluLysGluGluLysThrIleGlnAlaLeu65707580GlnValIleProIleSerSerGluAlaPheLeuAlaTyrArgLeuThr859095TrpAlaSerIleValThrAlaValLeuGlyPheAlaSerProTyrIle100105110LeuAspIleGluLeuSerArgLysGlyLeuAlaMetLeuIleValLeu115120125PheValLeuGluValTrpIleTyrGlyLeuLeuIleThrValPheSer130135140GluSerArgMetGlnAlaIleThrValSerLysValLeuGlyTrpPhe145150155160LeuMetLeuProProLeuIleLysLeuValValLeuTrpArgAsnLeu165170175SerArgAspTrpSerGluPheThrAlaPheLeuProThrTyrTrpLeu180185190TyrLysValPheGluGlyIleLeuGlnAsnAspTyrSerAspPheLeu195200205ValAlaPheIleValHisLeuIleTrpLeuIleProLeuIleAlaLeu210215220PheLysArgArgValLeu225230<210>145<211>240<212>PRT<213>Pyrococcusfuriosus<400>145MetArgArgIleLysValIlePhePheAspLeuAspAspThrLeuVal151015AspThrSerLysLeuAlaGluValAlaArgLysAsnAlaIleGluAsn202530MetIleArgHisGlyMetProValAspPheAspThrAlaTyrAsnGlu354045LeuLeuGluLeuIleLysGluTyrGlySerAsnPheProTyrHisPhe505560AspTyrLeuLeuArgArgLeuAspLeuGluTyrAsnProLysTrpVal65707580AlaAlaGlyValIleAlaTyrHisAsnThrLysPheThrTyrLeuArg859095GluValProGlyAlaArgLysThrLeuLeuArgLeuLysLysGluGly100105110TyrMetThrGlyIleIleThrAspGlyAsnProIleLysGlnTrpGlu115120125LysIleLeuArgLeuGluLeuAspAspPhePheGluHisValMetIle130135140SerAspPheGluGlyValLysLysProHisProLysIlePheLysLys145150155160AlaLeuLysAlaPheAsnValLysProGluGluAlaIleMetValGly165170175AspArgLeuTyrSerAspIleTyrGlyAlaLysAsnValGlyMetLys180185190ThrValTrpPheLysTyrGlyLysTyrAlaGluLeuAspLeuGluTyr195200205LysGluTyrAlaAspTyrValIleThrGluLeuProGlnLeuLeuGlu210215220ValLeuGluArgGluAsnGlySerAspLysGluValHisSerSerGly225230235240<210>146<211>218<212>PRT<213>Pyrococcusfuriosus<400>146MetIleLysGlyValIlePheAspValAspGluThrLeuValTyrTyr151015GluGlyTyrSerLeuLysGluTrpTyrGluLysIleAlaLysProThr202530MetLysLysLeuGlyIleLeuIleAspTrpGluThrPheArgLysMet354045AlaLysGlyGluLeuProArgSerTyrValGluGluPheGlyIleAsp505560HisValThrPheTrpLysAlaLeuAspLysAlaAsnArgArgTyrArg65707580GluGluLeuLeuAsnGluGlyLysIleArgThrTyrAspAspValGlu859095AlaIleLysGluLeuLysLysLeuGlyLeuLysLeuAlaAlaValSer100105110AsnAlaSerGlnAspAsnThrGluLeuValLeuLysAlaPheAsnLeu115120125LeuAspTyrPheAspValValTyrGlyLysAspTyrThrTyrLeuAsp130135140GlyValLysProAsnProTyrLeuIleAsnLysAlaLeuAsnThrLeu145150155160GlyLeuArgProAspGluAlaIleLeuValGlyAspSerAspLeuAsp165170175IleIleAlaGlyLysArgAlaGlyValThrValValGlnIleValArg180185190GluLysLysTyrGluGlyAlaAspTyrTyrIleGlnAsnLeuTrpGlu195200205LeuValAspLeuIleLysArgSerArgCys210215<210>147<211>234<212>PRT<213>Pyrococcusfuriosus<400>147MetIleLysAlaValPhePheAspPheIleGlyThrLeuLeuSerGln151015GluGlyGluTyrGluThrHisLeuLysIleMetGluGluValLeuGly202530GluAsnLysLysIleSerProGluGluLeuLeuArgGluTyrAspAla354045LeuThrArgGluAlaPheSerGlnTyrAlaGlyLysProPheLysPro505560IleArgIleIleGluGluGluIleMetLysLysLeuAlaGluLysTyr65707580GlyPheAspTyrProGluAsnPheTrpGluIleHisLeuLysAlaHis859095GlnArgTyrGlyLysLeuTyrProGluValValGluValLeuAsnGlu100105110LeuLysLysArgGluTyrHisValGlyLeuIleThrAspSerAspThr115120125AlaTyrLeuArgAlaHisLeuGluAlaLeuGlyIleAlaGluLeuPhe130135140AspSerIleThrThrSerGluGluAlaGlyPhePheLysProHisPro145150155160ArgIlePheGluValAlaLeuLysLysAlaGlyValLysGlySerGlu165170175AlaValTyrValGlyAspAsnProIleLysAspCysGlyGlyAlaArg180185190GlnLeuAspMetLeuSerIleLeuValAspArgLysGlyGluLysLys195200205GluLeuTrpLysGluCysGluPheValIleSerAspLeuArgGluVal210215220IleGlnIleValGluGluLeuAsnGlyGln225230<210>148<211>254<212>PRT<213>Pyrococcusfuriosus<400>148MetLysLeuLysPheTrpArgGluValAlaIleAspIleIleSerAsp151015PheGluThrThrIleMetProPhePheGlyAsnProAspGlyGlyLys202530LeuValLysIleSerProSerGlyAspGluThrLysLeuValAspLys354045LeuAlaGluAspLeuIleLeuSerArgIleThrGluLeuGlyValAsn505560ValValSerGluGluValGlyValIleAspAsnGluSerGluTyrThr65707580ValIleValAspProLeuAspGlySerTyrAsnPheIleAlaGlyIle859095ProPhePheAlaLeuSerLeuAlaValPheLysLysAspLysProIle100105110TyrAlaIleIleTyrGluProMetThrGluArgPhePheGluGlyIle115120125ProGlyGluGlyAlaPheLeuAsnGlyLysArgIleLysValArgLys130135140ThrProAspGluLysProSerIleSerPheTyrSerArgGlyLysGly145150155160HisGluIleValLysHisValLysArgThrArgThrLeuGlyAlaIle165170175AlaLeuGluLeuAlaTyrLeuAlaMetGlyAlaLeuAspGlyValVal180185190AspValArgLysTyrValArgProThrAspIleAlaAlaGlyThrIle195200205IleAlaLysGluAlaGlyAlaLeuIleLysAspSerAlaGlyLysAsp210215220IleAspIleSerPheAsnAlaThrAspArgLeuAspValIleAlaVal225230235240AsnSerGluGluLeuLeuLysThrIleLeuSerLeuLeuGlu245250<210>149<211>264<212>PRT<213>Pyrodictiumoccultum<400>149MetArgGlyArgGlyArgGlyArgLeuLeuGlyAlaValLeuAlaGly151015ValAlaSerAlaGlyGluLeuAlaArgLeuLeuProGlyArgValCys202530GlyValAlaValAspIleAspGlyThrIleThrGluArgArgValSer354045GlyAspPheArgLeuSerLeuGluAlaValGluAlaLeuArgArgLeu505560GluAspAlaGlyValArgValIleLeuValThrGlyAsnSerValMet65707580ValAlaAlaGlyValAlaArgTyrIleGlyValArgGlyProHisVal859095AlaGluAsnGlyCysLeuValTyrGlnArgGlySerValValHisAla100105110CysArgGlyThrAlaArgAlaAlaAlaGluAlaLeuGluGluGluLeu115120125GlyGlyLeuLeuGluProSerTrpGlnAsnArgCysArgIleHisAsp130135140TyrAlaPheLeuValArgArgValGlyProGluGluAlaTrpArgMet145150155160AlaGluArgValLeuArgGluArgGlyLeuArgAlaLysLeuSerHis165170175SerGlyTyrAlaLeuHisValArgProLeuGluAlaSerLysGlyLeu180185190GlyLeuArgValAlaMetArgMetAlaGlyLeuGluProGlyCysVal195200205ValAlaValGlyAspSerAlaMetAspLeuGluMetArgAspAlaGly210215220ValThrLeuAlaAlaValGlyAsnAlaAspProArgLeuArgGluGly225230235240AlaAspLeuIleLeuProGlyGluSerGlyGlyGlyValAlaLeuLeu245250255AlaArgAlaIleLeuGluSerLeu260<210>150<211>235<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>150MetHisLeuLeuLeuPheAspIleAspGlyThrLeuIleArgThrArg151015GlyPheGlyArgGlnThrMetGluAlaAlaLeuSerGluTrpLeuGly202530ArgProValThrThrGluGlyValAspPheAlaGlyArgThrAspPro354045AlaIleLeuLeuAspIleLeuLysAlaSerGlyLeuProGluHisThr505560AlaArgHisLeuLeuProGluAlaLeuGluValTyrSerArgAlaMet65707580IleArgArgLeuArgProGluHisLeuGluValLeuProGlyValVal859095MetLeuLeuGluGluLeuSerGluTrpProAspValTyrLeuGlyLeu100105110ValThrGlyAsnLeuArgProValAlaPheHisLysLeuAlaMetAla115120125GlyLeuAlaGlyTyrPheGlyGluGlyAlaPheGlyCysAspHisAla130135140AsnArgAsnGluLeuProProLeuAlaIleGluArgIleArgGluAla145150155160ThrGlyTyrProPheThrGlyAlaAspAlaValIleIleGlyAspThr165170175ProHisAspValAlaCysAlaArgHisAlaGlyAlaSerValAlaVal180185190ValCysThrGlyGlyTyrSerArgAspAlaLeuGluAlaCysArgPro195200205AspLeuLeuLeuGluAspLeuSerAspProGluProLeuPheLysLeu210215220LeuThrGlnGlnAlaLeuSerArgLysAlaSer225230235<210>151<211>279<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>151MetPheAspLeuArgArgAlaAspLeuHisLeuHisThrSerArgSer151015AspGlyArgLeuSerProAlaGluLeuValArgArgAlaArgGluAla202530GlyLeuTyrCysValAlaIleThrAspHisAspThrIleAspGlyLeu354045GluGluAlaArgGlnAlaAlaAlaArgTrpAlaMetValValIlePro505560GlyValGluLeuSerValGlnValGluGluGluGluValHisLeuLeu65707580GlyTyrPhePheAspProAspHisProAlaLeuArgGluAlaLeuThr859095AlaTyrArgLysAlaArgGluGluArgLeuAlaAlaMetLeuAlaArg100105110LeuGlnGluValGlyValArgLeuSerGluGluGlnValGlnThrAla115120125ValGlyHisGlyValProGlyArgProHisValAlaArgAlaLeuVal130135140AlaAlaGlyTyrAlaGluSerTyrArgGluAlaPheGlnArgTyrLeu145150155160LeuProGlyGlyProGlyTyrValProLysProAlaTrpThrAlaGlu165170175GluAlaValAlaValLeuHisGluAlaGlyGlyIleAlaValLeuAla180185190HisProGlyGluHisLeuArgAspArgValPheArgAlaLeuLeuGln195200205AlaGlyIleAspGlyIleGluValIleHisProAlaHisSerTyrTyr210215220LeuValGlnHisTyrArgGlnValAlaArgAspPheGlyLeuLeuGlu225230235240ThrGlyGlySerAspTyrHisGlyHisArgProGluAspAspAlaLeu245250255LeuGlyAlaCysThrIleProTyrProArgValGluArgLeuArgAla260265270ThrLeuGlnAlaThrArgAla275<210>152<211>846<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>152MetSerSerArgArgPheAlaThrSerAspLeuValLeuAlaLeuAla151015GlyValIleGlyLeuValLeuGlyLeuTrpLeuLeuProArgGlnHis202530ProAspAlaAlaLeuGlnHisMetLeuSerAlaGluAlaAlaArgHis354045ArgAlaAlaAlaPheLeuAlaGlnArgGlyTyrArgValAspThrThr505560HisAlaLeuValValLeuArgArgAlaProGluLeuLeuArgArgTrp65707580GlnThrArgTrpGlyArgProGluLeuValArgArgLeuGluAspLeu859095ProTrpLeuProValTyrArgTrpValValTyrArgSerSerAspGlu100105110MetAsnGlyGlnArgTrpGlnValValLeuAlaGlyAspGlyThrIle115120125TrpGlyPheArgGlyProGluThrProGlyGluAspProAlaAlaArg130135140AlaAlaValGlyIleAlaAlaGluAlaPheAspProLeuAlaSerTrp145150155160GlyLysLeuSerGlyAlaProAspSerSerGluThrProGluArgPro165170175GlyProAlaAlaAlaValAlaLeuAlaArgTyrHisLeuGlnArgThr180185190IleGlySerValLeuProLeuArgProAspSerValGlyLeuLeuAla195200205ThrSerSerProGlnGlnAlaValValArgPheArgGlyLeuSerPro210215220ThrGlyAspSerValThrValGlnValValValThrAlaSerGlyGln225230235240LeuArgAlaLeuGluAlaThrTrpGlyThrLeuHisLeuProGluPro245250255SerProLeuGluGluGlyProProArgArgGlyGluArgGlyPheThr260265270ValHisGluValGlnAspLeuLeuSerValLeuLeuPheAlaGlyLeu275280285GlyIleTrpLeuLeuThrValPheLeuArgArgLeuHisArgArgLeu290295300LeuAspThrGlnGlyProLeuArgAspAlaValLeuGlyGlyLeuAla305310315320PheThrValAlaThrLeuGlyGlyAlaLeuProGlyIleLeuGlnVal325330335ProAspLeuTrpLeuArgLeuLeuIleLeuLeuMetSerThrLeuVal340345350ValGlyValPheGlyAlaAlaSerValPheLeuLeuAlaGlyThrSer355360365AspAlaLeuAlaArgAspArgTrpProGluLysLeuAlaValLeuThr370375380LeuLeuArgAsnGlyGlnValArgAsnValValValGlyAlaSerLeu385390395400LeuArgGlyLeuAlaLeuGlyGlyLeuLeuLeuGlyMetThrValGly405410415LeuLeuGlyLeuTrpProArgAlaAlaLeuArgLeuAspAlaGlyAla420425430TrpThrValProTrpProGlyMetGlnAlaAlaValTrpLeuGlyPhe435440445AlaValTrpValGlyMetLeuLeuValTyrLeuLeuLeuAlaValMet450455460AlaArgLeuProTrpArgAspGlyGlyArgValValGlyThrLeuThr465470475480LeuLeuLeuLeuLeuAlaGlyValSerAlaIleAspLeuGluGlnVal485490495GlyPheGluLeuLeuValHisAlaLeuTrpGlyValValLeuAlaTrp500505510AlaCysTrpArgTyrGluProAlaCysSerGlyIleGlyAlaMetThr515520525AlaTrpAlaLeuTrpArgSerAlaSerGlyTrpMetAlaProAspGly530535540ProPheGlyProAspGlyTrpIleValLeuAlaMetValGlyThrGly545550555560LeuValIleGlyPheValGlyIleArgSerArgArgSerAlaAlaGlu565570575LeuProArgTyrValProAlaTyrLeuGlnGluLeuAlaArgGlnGlu580585590ArgLeuGluArgGluLeuGluIleAlaArgGlnAlaGlnAlaSerLeu595600605LeuProArgThrLeuProGluValProGlyAlaAlaMetAlaAlaLeu610615620CysArgProAlaTyrGluValGlyGlyAspTyrTyrAspValPheAla625630635640LeuProAspGlyArgLeuAlaValValValGlyAspValSerGlyLys645650655GlyIleGlnAlaAlaPhePheMetThrLeuIleLysGlyHisValArg660665670AlaLeuSerLeuSerThrArgAspProAlaAspValLeuArgHisLeu675680685AsnArgLeuPheArgGluGlnAlaProArgGlyLeuPheValThrMet690695700IleTyrGlyValLeuAspProThrThrArgThrPheThrLeuAlaArg705710715720AlaGlyHisProProValLeuHisTyrArgAlaCysThrGlnGlnVal725730735GlnCysLeuArgProSerGlyMetGlyIleGlyLeuAlaAspAlaGlu740745750LeuPheAspLysAlaLeuGluAsnCysValLeuArgLeuGluAlaGly755760765AspArgValLeuLeuTyrThrAspGlyIleThrGluMetAlaGlyPro770775780ArgGlyGluArgTrpGlyLeuGluArgLeuGlnGlnTrpLeuArgGlu785790795800SerSerArgArgGlyTyrAlaProGluGlnAlaLeuGlyAlaLeuGlu805810815GluGlnLeuArgAlaPheAlaGlyThrThrGluLeuAlaAspAspLeu820825830ThrAlaIleLeuLeuGluAlaLysGlnAspGlyTyrAlaAsn835840845<210>153<211>534<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>153MetProAlaValValAlaGluLeuGluGlnAlaThrArgSerProVal151015ArgMetCysValIleAspLeuGlyThrAsnSerPheHisAlaValIle202530ValAspAlaLeuProGlyThrPheArgValValAspArgPheLysGlu354045MetValArgLeuGlyGluGluGlyLeuGlnGlyGlnArgLeuSerGlu505560AlaAlaMetGlnArgAlaIleArgAlaLeuArgArgIleArgGlnLeu65707580AlaGluGlyTrpGlyAlaThrGluPheLeuAlaCysAlaThrSerAla859095IleArgGluAlaGluAsnGlyGlyGluLeuLeuGlnArgIleArgSer100105110GluValGlyLeuHisValArgValIleAspGlyLeuGlnGluAlaArg115120125LeuIleTyrLysGlyValArgArgAlaValProMetProGluProVal130135140LeuIleMetAspValGlyGlyGlySerValGluPheIleValGlyThr145150155160SerGlnGlnProLeuHisLeuPheSerLeuLysLeuGlyAlaAlaArg165170175MetThrArgArgPheValArgHisAspProAlaThrArgGluGluLeu180185190LysAlaLeuArgAlaPheTyrArgGluGlnLeuHisProValPheGlu195200205AlaAlaArgAlaHisGlyValArgGluValValGlySerSerGlyAla210215220MetGluAsnLeuAlaSerValCysAlaArgMetArgGlyGluValAla225230235240ArgSerIleTyrGluGlnProPheProAlaGluAspPheArgArgVal245250255AlaArgArgIleMetArgLeuSerArgGluGlnArgArgArgLeuArg260265270GlyIleAspProLysArgValAspGlnIleValAlaAlaAlaAlaLeu275280285IleAspValValLeuLysAspLeuAlaIleGluArgValArgIleSer290295300ProHisAlaLeuArgGluGlyLeuValValAspPheValGluArgAsn305310315320AlaProLeuLeuAlaArgLeuThrAlaPheAlaAspValArgArgArg325330335SerIleTyrGluMetGlyTrpArgPheAspTrpGluTyrArgHisAla340345350GlnGlnValAlaAlaLeuAlaLeuGlnLeuPheAspAlaThrGlnSer355360365LeuHisGlyLeuGlyHisValAspArgGluLeuLeuGluTyrAlaAla370375380LeuLeuHisAspIleGlyTyrHisIleSerHisArgSerHisHisLys385390395400HisGlyLeuTyrLeuIleLysHisAlaAspLeuArgGlyPheThrSer405410415GluGluIleAlaValLeuAlaAsnValValArgTyrHisArgGlySer420425430LeuProLysProThrHisAlaAspTyrMetAlaLeuSerGluGluAsn435440445ArgValArgValCysLysLeuAlaAlaLeuLeuArgLeuAlaGluGly450455460LeuAspArgSerHisAsnGlnAsnValArgThrLeuHisValArgLeu465470475480GluSerAspArgLeuValLeuHisLeuGluThrArgGlyAspProGlu485490495LeuGluValTrpGlyValArgArgSerAlaGluLeuPheGluGlnThr500505510PheGlyArgThrValGluValSerAlaThrAlaGluProProLeuLeu515520525GluGluThrAlaGlyAla530<210>154<211>336<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>154MetThrThrAsnHisIleGlnGluLeuLeuSerLysLeuAsnGluLeu151015ArgAlaValPheIleLeuGlyGlnArgAlaIleProPheIleGluGlu202530ValValTyrPheLeuArgGluIleSerProLeuLeuSerGluValAsn354045AspSerLeuAlaGluSerThrArgLysMetProArgAlaSerSerGln505560LeuLysSerValThrGlnAlaThrGluMetAlaThrThrGluIleLeu65707580AspLeuIleAspValValLeuAsnAspLeuAspThrPheArgLysSer859095TrpAlaAlaAlaProGluAlaLeuGlnThrLeuGlnSerGlnGluGlu100105110GlnLeuTrpGlnProLeuProGluSerTrpAsnGluArgLeuGlnThr115120125LeuLeuGluGluArgArgGlnHisTyrAlaThrLeuGlnAlaMetLeu130135140GluSerGlnGlnGlnLeuValAspSerLeuArgAspArgMetAsnArg145150155160IleMetMetAlaLeuGlnValGlnAspIleThrAlaGlnGlnLeuAla165170175AlaValAsnHisLeuIleGluSerValArgTyrArgMetAlaGlnLeu180185190IleArgArgLeuGlySerGluValLeuAspAspLeuGluLeuProArg195200205GlnLeuPheAlaGluGlyThrPheAspProHisAlaArgTyrAspArg210215220AspGlyArgArgGlnGlnGlnValAspAlaLeuValAspAlaLeuGln225230235240HisAsnAlaSerLeuProGluAspGlyGluSerThrGlyProAlaSer245250255GlnAspGluIleAspAlaLeuPheSerGlyAsnSerThrProAlaSer260265270GlnAspGluIleAspSerLeuPheSerSerGlyThrProThrSerGln275280285AspGluIleAspAlaLeuPheGlyGlyGlySerThrProAlaSerGln290295300AspGluIleAspAlaLeuPheGlyGlyGlySerThrProAlaSerGln305310315320AspGluIleAspAlaLeuPheAsnProLysArgAsnAspSerAsnSer325330335<210>155<211>231<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>155MetSerLeuLeuPheThrGluGluGluLeuThrArgValArgGluAla151015ValAlaAlaAlaGluGlnArgThrAlaGlyGluIleValProValIle202530ValProArgSerGlyAlaTyrProGluAlaValTrpLysGlyAlaVal354045LeuLeuMetLeuProValLeuAlaValAlaLeuLeuPheAspTyrIle505560TyrGlnGlyTrpGlyLeuThrLeuLeuHisThrGlyTrpGlyValAla65707580LeuLeuThrAlaLeuAlaGlyLeuValGlyGlyLeuLeuGlyAlaTyr859095ValAlaProValGlnArgTrpLeuValGlyGluAlaArgMetAlaGlu100105110GlnValHisLeuArgAlaLeuGlnAlaPheLeuGluGluGluValPhe115120125AsnThrArgAspArgThrGlyIleLeuIlePheValSerLeuPheGlu130135140HisTrpValGluValIleGlyAspAlaGlyIleAsnGlnArgValGly145150155160ProGluAlaTrpAlaGluValValAspArgIleArgLysGlyIleArg165170175GluGlyArgProValAspGlyLeuValAlaAlaIleGluGlnCysGly180185190GlnLeuLeuAlaThrHisGlyValAlaLeuArgProAspAspThrAsn195200205GluLeuAlaAspThrLeuArgValArgGlyAlaProSerLysLysArg210215220ArgGlyGlyArgArgLysArg225230<210>156<211>221<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>156MetProAlaThrThrLeuTyrPheValArgHisGlyGluThrAspTyr151015AsnArgAsnGlyIleValGlnGlyArgGlyValAspAlaProLeuAsn202530GluArgGlyArgArgGlnAlaGluAlaLeuAlaArgArgPheAlaAla354045ValProLeuAspAlaIleTyrAlaSerProLeuArgArgAlaLeuGlu505560ThrAlaGluAlaValArgArgTyrHisProGluValProPheTyrGln65707580LeuAlaAspLeuGluGluMetAspTrpGlyAspLeuGluGlyLysPro859095TyrAlaProProTyrAspAlaLysIleArgAlaIleTyrGluArgTrp100105110ArgAlaGlyAspTyrAspTyrProValProGlyGlyGluSerIleLeu115120125AspValGlnArgArgAlaLeuArgAlaLeuGluThrIleLeuSerArg130135140HisGluGlyGluThrValLeuIleValAlaHisGlyArgPheLeuArg145150155160IleLeuLeuAlaSerValLeuSerGluTyrGlyLeuAlaArgMetGlu165170175AlaLeuProHisThrAsnThrAlaValAsnHisLeuValTyrGluAsn180185190GlyArgPheArgAlaLeuArgLeuAsnCysThrAlaHisLeuGluGlu195200205AlaAlaValAspAspGlyLeuProThrGluValAlaAla210215220<210>157<211>162<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>157MetArgLeuCysLeuLeuArgHisAlaGluAlaTyrProAlaAlaPro151015GlyArgProAspAlaGluArgSerLeuThrGluValGlyGlnGlnVal202530AlaArgGlnMetGlyGluAlaLeuArgArgLeuArgLeuAlaProGly354045AlaValTyrThrSerProTyrArgArgAlaValGlnThrAlaGlnAla505560ValAlaGluAlaLeuGlyValProValValGluAspArgLeuLeuAla65707580ProGlyCysGlyProAlaGluLeuGluThrLeuIleGlnAlaTyrAla859095ProGlyGluThrValLeuValValGlyHisGlnProAspPheGlyGlu100105110LeuValArgTrpLeuThrGlyAlaThrIleArgLeuProAlaGlyGly115120125LeuAlaValValGluThrProAlaLeuArgGluArgAlaGlyThrLeu130135140HisGlyLeuTyrAspProAlaTrpLeuAlaAlaValMetThrGlyArg145150155160ThrGly<210>158<211>249<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>158MetAsnValGluValPheLeuThrGlySerHisValThrGluGluAsp151015ValGluGlyArgThrValValValIleAspValLeuArgAlaSerSer202530ThrIleIleThrAlaLeuAlaAsnGlyAlaArgAlaValIleProVal354045AlaAspMetAspGlnAlaGlyLysIleAlaMetAsnLeuAspProSer505560ThrTyrLeuLeuGlyGlyGluArgAspGlyAspArgIleAspGlyTyr65707580HisLeuGlyAsnSerProLeuGluTyrThrProAspValValGluGly859095LysThrIleIleLeuAsnThrThrAsnGlyThrArgThrIleTrpAsn100105110AlaArgAsnAlaGluHisLeuIleValGlyGlyPheLeuAsnAlaAsn115120125ArgValValGlnPheValArgGluAlaGlyLeuAspValThrIleIle130135140CysAlaGlyArgAsnAsnArgValAlaLeuAspAspAlaLeuCysAla145150155160GlyLeuLeuLeuHisArgLeuTrpGluGlyArgGluProGluTyrVal165170175SerAspAlaAlaHisIleAlaLeuThrGlnTyrLeuHisAspArgAsp180185190ArgLeuAlaAspAlaLeuArgHisSerAsnHisAlaArgTrpLeuIle195200205GluLysGlyTyrGlyAlaAspValGluTyrCysLeuGlnLeuAspAla210215220LeuProValLeuProTyrTyrArgGluAsnArgLeuValLeuTyrArg225230235240GluArgGlnProSerGluSerValGly245<210>159<211>345<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>159MetGluThrLeuHisThrArgGlyArgLysValValGluGluPheIle151015ThrLeuGluGlnPheIleIleAspGlnGlnGluArgPheProHisSer202530ThrGlyAlaPheSerArgLeuLeuArgAspIleSerValAlaAlaLys354045IleValAsnArgAspIleArgArgAlaGlyLeuValAspIlePheGly505560ThrThrGlyLysValAsnIleHisGlyGluValGlnGlnLysLeuAsp65707580AlaLeuAlaHisGluGluPheValArgAlaLeuArgArgGlyGlyGlu859095CysCysLeuIleGlySerGluGluHisAlaGluAlaIleProLeuSer100105110AlaAsnGlyGluGlyAspGlyArgTyrIleValLeuLeuAspProLeu115120125AspGlySerSerAsnValAspValAsnValSerValGlyThrIlePhe130135140SerIleTyrArgLeuProAspGluTyrGluThrProThrLeuGluAla145150155160AlaLeuGlnProGlySerLysGlnValAlaAlaGlyTyrIleValTyr165170175GlySerSerThrMetLeuValTyrThrThrGlyAsnGlyValAsnGly180185190PheThrLeuAspProSerIleGlyGluPheIleLeuSerHisProAsn195200205IleArgIleProLysThrGlySerIleTyrSerIleAsnGluGlyAsn210215220PheAsnSerPheGluGluGlyLeuLysArgPheIleArgTrpAlaGln225230235240GluGluAspLysAlaThrGlyArgProPheSerThrArgTyrIleGly245250255SerPheValSerAspPheHisArgAsnLeuLeuLysGlyGlyIleTyr260265270MetTyrProAlaThrLysLysAsnProGluGlyLysLeuArgLeuMet275280285TyrGluAlaAsnProMetAlaPheIleValGluGlnAlaGlyGlyArg290295300AlaSerAspGlyHisArgArgIleLeuAspIleValProGluLysLeu305310315320HisGlnArgThrProLeuPheIleGlySerGluGluLeuValArgThr325330335ValGluGluPheLeuGlnGlyLysArg340345<210>160<211>197<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>160MetLysArgArgGlyLeuTrpSerGlyThrValLeuAlaValLeuIle151015ThrAlaSerAlaGlnAlaGlnThrAlaCysAspArgProAlaLeuGlu202530LeuArgLeuLeuCysThrValTyrArgTrpAspGlyProValAlaSer354045AlaTyrPheGluThrIleAspAlaThrAlaTyrProMetPheAlaGly505560LeuThrAlaMetAlaTrpGlyGlyValLeuAlaGlyArgLeuGluArg65707580ProAlaAlaGluArgValThrLeuAlaThrAlaAlaThrThrValLeu859095ValPheGlyLeuLysAlaLeuAlaArgArgAsnArgProPheAspAla100105110TrpAspAspIleAlaProArgGlyAspProProThrSerHisAlaPhe115120125ProSerGlyHisAlaAlaLeuAlaPheThrLeuAlaThrAlaTrpGly130135140LeuGluValProArgIleTyrValIleValProAlaTyrValTrpAla145150155160ThrSerValAlaValGlyArgValTrpLysGlyValHisTyrProThr165170175AspValLeuAlaGlyAlaValLeuGlyAlaGlyValAlaTrpThrVal180185190HisArgIleTrpArg195<210>161<211>269<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>161MetSerTrpTrpGluAlaLeuIleLeuGlyLeuLeuGlnGlyLeuThr151015GluPheLeuProValSerSerSerGlyHisLeuValLeuGlyGlnTyr202530ValLeuGlyLeuAsnProGlyGlyValThrPheGluValPheValHis354045PheGlyThrValLeuSerIleLeuThrValTyrArgLysArgValGly505560AlaIleValGlyGluValTrpThrAlaLeuProArgProAlaGluTrp65707580ProMetArgTyrArgGluArgAspProPheArgLeuAlaValTrpIle859095LeuIleThrMetIleProThrGlyLeuValTyrValLeuLeuGlyAsp100105110TrpIleGluGlnThrPheGluHisProArgPheAlaAlaGlyMetLeu115120125ValValThrGlyValLeuLeuIleLeuThrArgLeuArgArgHisPro130135140AspGlyAspLeuSerProLeuLysAlaPheValValGlyValAlaGln145150155160SerAlaAlaMetLeuProGlyIleSerArgSerGlySerThrIleCys165170175AlaAlaIleTyrGlnAsnValArgProGluArgAlaAlaAspPheSer180185190PheLeuMetLeuLeuProValValLeuGlyAlaThrLeuLeuLysGly195200205IleGluLeuLeuAsnSerProGluSerIleGlyMetGlyProLeuVal210215220LeuGlyThrValAlaAlaTyrValSerGlyValAlaAlaIleArgMet225230235240LeuLeuGlnValValArgArgGlyArgLeuGluTyrPheAlaTyrTyr245250255CysPheLeuValGlyLeuLeuGlyLeuTrpLeuIleArg260265<210>162<211>366<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>162MetSerThrArgArgAlaValLeuAsnCysPheArgArgHisGlnArg151015PheValLeuThrThrHisIleLysProAspGlyAspAlaLeuGlySer202530GlnLeuAlaLeuGlyArgLeuLeuGlnLysMetGlyHisAspValTyr354045LeuIleAsnSerAspProProProSerAsnLeuThrTrpIleProGly505560IleGluGlnValGluValPheAsnGlyAlaLeuAlaGlnArgGluArg65707580IleAspGlnAlaAspValIleCysValLeuAspThrAsnAlaLeuAsp859095ArgLeuGlyAspLeuAlaProAlaValGluAlaSerArgAlaArgLys100105110LeuLeuIleAspHisHisThrSerProGluAspTrpPheAspLeuGln115120125TyrValArgAspThrAlaSerSerThrGlyGluLeuValTyrGluLeu130135140ValCysAlaValAspProAsnLeuIleAspHisGluLeuAlaThrAla145150155160LeuTyrValAlaIleMetThrAspThrGlySerPheArgPheAsnThr165170175ValThrProThrValHisArgIleValAlaAspLeuLeuGluArgGly180185190GlyLeuSerThrGluAlaIleHisSerAlaIlePheAspThrArgThr195200205ProGluSerMetArgLeuLeuGlyLeuAlaLeuArgAsnLeuGlnLeu210215220ArgTyrAspGlyArgValAlaTyrMetValLeuSerArgArgMetPhe225230235240AsnGluThrGlyAlaSerThrGluAspThrGluGlyPheIleAsnHis245250255LeuLeuSerIleArgGlyValArgValAlaLeuLeuPheThrGluIle260265270GluLysGlyValLysIleSerPheArgSerLysGlyAspTyrHisVal275280285AsnGluTrpAlaArgAlaPheGlyGlyGlyGlyHisArgAsnAlaAla290295300GlyAlaPheValGluAsnAlaAlaLeuAspAlaLeuValAspAlaVal305310315320LeuAlaAlaAlaProArgTyrLeuProGlnLeuGluAlaSerSerAla325330335GlyAsnAlaSerGlyThrLeuSerSerGluAspAlaSerTyrLeuSer340345350AlaLeuLeuHisGlnLysSerGlnAlaSerSerThrAlaSer355360365<210>163<211>174<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>163MetSerGlnThrAsnHisGlnProIleArgValLeuPheValCysLeu151015GlyAsnIleCysArgSerProLeuAlaGluGlyValPheArgLysLeu202530ValAspGluAlaGlyLeuThrAlaHisPheGluIleAspSerAlaGly354045ThrGlyProTrpHisValGlyGluProAlaAspArgArgMetGlnArg505560ThrAlaArgArgHisGlyValAspLeuSerGlyHisValAlaArgGln65707580LeuGlyArgGluAspLeuAlaArgTyrAspHisIleTyrValMetAsp859095ArgGluAsnLeuGluAspValLeuArgLeuAspArgAspGlyArgPhe100105110ArgHisLysValGluLeuPheArgThrPheAspProGluProGlyAsp115120125GlyGluValProAspProTyrTyrGlyGlyGluArgGlyPheGluGlu130135140ValTyrGlnIleValGluArgThrAlaArgArgLeuLeuGluHisLeu145150155160ValSerLeuTyrLysLeuLysGluThrAlaAspLeuSerArg165170<210>164<211>272<212>PRT<213>海洋红嗜热盐菌(Rhodothermusmarinus)<400>164MetMetAspAlaTyrThrLeuTyrGluGluAlaArgGluValAlaAla151015ArgLeuAlaArgAspAlaGlyGlnIleAlaArgTyrTyrAlaGlyArg202530ValThrValArgGluLysGlyTyrAsnGluLeuValThrGlnAlaAsp354045GluGluValGlnArgPheLeuIleGluGlnIleHisArgHisPhePro505560GluHisAlaIleLeuAlaGluGluAsnLeuSerAspMetGlnAspGly65707580ArgGluGlyAlaSerPheArgTrpIleIleAspProIleAspGlyThr859095ThrAsnPheThrHisGlyValProProTyrGlyIleSerLeuAlaLeu100105110GlnHisGluGlyArgThrValValGlyValValTyrAspValProHis115120125AspGluLeuPheThrAlaValArgGlyGlyGlyLeuTyrValAsnGly130135140ValArgAlaArgValSerGlnThrGluThrLeuArgGluAlaLeuIle145150155160ThrThrGlyPheProTyrArgGluValValHisLeuGluGluTyrLeu165170175GluAlaLeuGlyArgValIleArgAlaThrArgGlyValArgArgPro180185190GlyAlaAlaSerValAspLeuAlaTrpValAlaCysGlyArgPheAsp195200205GlyPhePheGluThrGlyLeuSerProTrpAspValAlaAlaGlyIle210215220LeuLeuValGluGluGlyGlyGlyArgValThrAspPheHisGlyArg225230235240ProAspProIlePheAlaArgGlnMetLeuAlaThrAsnGlyArgIle245250255HisGluAlaLeuCysGluLeuValAlaProLeuHisHisValTyrAla260265270<210>165<211>219<212>PRT<213>Slackiaheliotrinireducens<400>165MetIleLeuLysAlaAlaLeuPheAspAsnAspGlyThrLeuValAsp151015SerGluGluLeuIleLeuSerSerPheArgTyrAlaThrLysSerVal202530LeuGlyGluAlaLeuProAspGluValLeuArgArgLysValGlyGln354045ProLeuArgThrGlnMetAlaAspPheThrProAspValAspLysArg505560GluGluLeuPheArgValTyrGlnGluPheAsnAlaArgGluHisAsp65707580ArgMetIleArgLeuPheProAspValAlaAsnThrLeuGlyThrMet859095LeuGlnArgGlyLeuArgLeuGlyValValThrSerLysLeuSerGlu100105110AsnCysLeuGlnAsnLeuSerHisLeuGlyIleAspGlyTyrPheGlu115120125CysIleValAlaProAspAsnCysProLeuHisLysProAspProGly130135140ProValLeuGluGlyAlaLysLeuLeuGlyAlaArgProGluGlnCys145150155160ValTyrValGlyAspSerProTyrAspIleAlaAlaGlyArgAspAla165170175GlyCysThrThrIleAlaValThrTyrGlyValPheSerArgGluAsp180185190LeuLysProGluArgProAspTyrPheCysAspSerPheAlaGluLeu195200205LeuSerValLeuAspGlyIleValArgArgGly210215<210>166<211>264<212>PRT<213>Slackiaheliotrinireducens<400>166MetLeuAlaHisGlnAsnGluThrMetThrLysTyrLeuPhePheAsp151015IleAspGlyThrLeuAlaAlaGlyProIleGlyAsnArgValValPro202530GluGlyThrArgAspAlaLeuLeuArgLeuArgLysAsnGlyHisPhe354045ThrAlaIleCysThrGlyArgSerHisAlaMetAlaArgSerTyrMet505560GluGluGlnGlyPheThrAsnMetValAlaAspGlyGlyAsnSerThr65707580ValIleAspGlyAsnLeuLeuGlyIleGluProLeuAspArgLysArg859095CysIleGlnValLeuGluGluCysAspArgLeuLysIleProTrpAla100105110ValSerAlaAspAspSerAsnThrArgLeuSerIleAspGlyArgPhe115120125AlaAspLeuValGluLysGlyTyrMetValThrLysThrValProAsn130135140LeuAspTyrHisAlaIleProArgPheTyrLysLeuTyrIleAlaVal145150155160AsnAlaGluAspGluLysArgIleThrThrLeuAspLeuValProHis165170175ValHisPheSerAspThrCysLeuPheValGluProIleAspLysSer180185190ArgGlyIleMetArgIleMetGluHisLeuGlyAlaProIleGluAsp195200205ValValValPheGlyAspGlyMetAsnAspLeuSerMetPheAspPro210215220ArgTrpLeuSerIleAlaMetGlyAsnAlaValGlnProLeuLysAsp225230235240AlaAlaAspTyrIleThrAlaAsnAlaAspAspAspGlyIleArgLys245250255AlaCysGluHisPheGlyTrpIle260<210>167<211>238<212>PRT<213>海葡萄嗜热菌(Staphylothermusmarinus)<400>167MetIleArgLeuAlaAlaPheAspIleAspGlyThrLeuThrIleAsn151015ArgSerSerThrValLeuCysLeuGluAlaIleAspAlaLeuArgLys202530LeuGluLysAsnGlyValIleValValLeuValSerSerAsnAlaLeu354045ProValValValGlyLeuLysLysTyrIleGlyLeuSerGlyProAla505560IleGlyGluThrGlyAlaLeuIleTyrTyrGlyGluGluGluIleVal65707580AlaThrThrLysTyrSerAlaLysGlnAlaTyrLeuAspValLeuGlu859095LysTyrAsnGluTyrValTyrGlySerTrpGlnAsnMetPheArgLeu100105110HisAspTyrAlaLeuLysIleArgLysGlnTyrLeuSerLysAspAsn115120125GluIleTyrSerLeuIleLysGluTyrValGluAsnLysTyrProTyr130135140IleLysValGlyTyrSerGlyTyrAlaIleHisLeuThrProLysAsp145150155160ThrGlyLysGlyLysAlaLeuLysGlnIleMetGluLysHisGlyIle165170175ArgArgGluGluThrMetGlyValGlyAspSerIleMetAspTrpGlu180185190PheIleLysGluThrLysIleLysValAlaValAlaAsnAlaAspPro195200205GluLeuArgArgLysAlaAspIleValThrThrLysProSerGlyTyr210215220GlyValValGluIleValGluLysIleLeuAspLysProPro225230235<210>168<211>227<212>PRT<213>海葡萄嗜热菌(Staphylothermusmarinus)<400>168MetAlaArgIleLysAlaValLeuLeuAspTyrAspLeuThrLeuMet151015AsnAsnLeuIleAspPheTyrAspAlaTyrAsnGluAlaLeuLysLys202530PheValGlyLysThrLeuGlyPheAsnGluPhePheHisLeuLeuIle354045AsnTyrSerLeuGlnAlaTyrIleProProAspValAspArgPheSer505560PheTrpArgTyrPheArgGlnValTyrArgThrArgTyrGlyTyrPro65707580MetGluGlyAlaTyrTyrPheLeuTyrTrpValLysAlaLeuGlyLeu859095LysThrIleIleValSerGlyArgGluCysHisGluSerSerIleTrp100105110GluGluLeuLysArgPheGlyLeuAsnGluTyrIleAspLysValTyr115120125ThrMetPheAspThrLeuIleLeuGlyGlyValGluGluGluLeuPhe130135140AspLysThrTrpLeuIleSerTyrAlaLeuSerSerTyrGlyLeuAsp145150155160ArgAspGluValValTyrIleGlyAspTyrArgGlnAspLeuLeuSer165170175SerGlnArgThrGlyIleLysPheIleGlyIleAlaPheSerGluLys180185190ArgLysGluCysLeuArgArgLeuGlyAlaGluTyrValGlySerAsn195200205LeuTyrAspValThrTyrTyrLeuTrpGlnIleIleArgGluValGlu210215220PheLysLys225<210>169<211>222<212>PRT<213>海葡萄嗜热菌(Staphylothermusmarinus)<400>169MetThrSerThrGluValValLysAlaValLeuPheAspMetAspGly151015ThrIleIleAsnSerValGluLeuIleAlaGluCysTrpSerLysAla202530PheLysLysHisGlyIleArgIleGluProGlnAspIleTyrArgVal354045ValGlyLeuProAlaAspThrIleLeuGluLysTyrThrGlyThrLys505560AsnProArgLeuHisAsnSerIleLeuGluGlnAlaArgLysCysPhe65707580GluGluLysMetAsnProAsnThrLeuLeuTyrAsnAspValLeuGlu859095ThrIleLysGlnLeuArgGluAsnAsnLysLeuCysGlyIleValThr100105110SerSerSerCysLysArgThrIleGluLeuLeuGluLysLeuAspIle115120125IleGluTyrPheAspThrIleGlnCysTyrGlnGlyLysLeuArgGly130135140LysProTyrProAspLeuLeuLeuSerAlaLeuAsnLysLeuGlyIle145150155160LysProSerGlnAlaIleTyrValGlyAspSerTyrIleAspTyrLeu165170175ThrAlaArgAsnThrGlyValLeuPheValLeuValLysArgSerTrp180185190AsnThrHisIleValAspLysCysSerGluLysCysIleValIleSer195200205AspLeuArgGluIleSerAsnLeuIleThrAsnLysIleLys210215220<210>170<211>225<212>PRT<213>嗜酸热硫化叶菌(Sulfolobusacidocaldarius)<400>170MetAsnLysAspIleIlePheValSerAspTyrAspArgThrLeuSer151015SerGluLysAsnAsnPheArgIleAspLysGluValAlaArgIleVal202530AsnAspPheSerLysThrTyrProPhePheValValThrGlyArgGlu354045LysLysPheIleAspIleLeuAlaProGluLeuLysProThrGlyTrp505560IleLeuGluAsnGlyAlaLeuMetTyrValAsnGlyGluLeuIleTyr65707580AsnIleGlnProSerTrpPheAspThrArgLysAsnIleIleLysIle859095LeuAspAsnTyrAsnIleSerTyrSerLeuGlyAsnValIleValTyr100105110ValAspLysAlaHisGluTyrLysGlyValLeuAsnSerIleLysAsp115120125AlaThrValGluTrpAsnArgAsnAspAlaMetIleLeuProLysGly130135140ValAspLysGlySerAlaValIleGlnLeuArgGluArgLeuGlyTyr145150155160ArgGlyLysIleValAlaIleGlyAspSerGluAsnAspIleSerMet165170175PheArgValAlaAspIleArgValSerValAlaAsnAlaLeuProMet180185190IleLysGluIleSerGluLeuIleLeuGluLysGluAspGlyGluGly195200205ValLysGluPheLeuLeuLysValLeuLysGlyGluIleIleLeuIle210215220Lys225<210>171<211>224<212>PRT<213>嗜酸热硫化叶菌(Sulfolobusacidocaldarius)<400>171MetIleTyrAsnIlePheIlePheValIleLysTyrAsnPheProVal151015PheLysValLysGlyValIlePheAspLeuAspGlyThrLeuAlaAsn202530ThrAlaLeuIleHisLysGluAlaTrpGluLysAlaLeuAspArgLeu354045GlyIleLysSerAspValLysIleAspAsnLeuLeuGlyArgLysSer505560SerAspIleAlaLysLeuLeuAlaProAsnAsnTrpSerGluLeuIle65707580AsnIleLysAsnGlnIleTyrValGluLeuValLysGluLysAlaSer859095ProThrProCysAlaLeuAspLeuLeuSerTyrLeuArgLysLysGlu100105110IleLysThrAlaIleValThrSerSerAsnLysLeuSerSerGlySer115120125ValLeuLysLysLeuGlyIleThrSerGluValValLeuThrGlyAsp130135140AspValIleAsnSerLysProAsnProGluGlyIleAsnLysAlaLeu145150155160SerLeuLeuTyrLeuAspGlyArgAspValIleGlyValGlyAspThr165170175GluValAspValGluAlaTyrTyrLysAlaGlyLeuGlyGlyIleTyr180185190LeuValLysSerGlyValProPheArgGluGluValValLysMetTyr195200205GlyGlyIleValIleSerSerLeuCysGluLeuLeuGluLeuIleSer210215220<210>172<211>267<212>PRT<213>嗜酸热硫化叶菌(Sulfolobusacidocaldarius)<400>172MetLysArgGluAspValGluLysValAlaSerGluAlaSerLysTyr151015IleTyrGluGluArgGluAsnLysAspValAspArgValIleAsnVal202530HisGlyAsnAspValThrArgIleIleAspLysArgSerGluAspPhe354045IleValAspArgLeuLysSerLeuGlyTyrAsnIleLeuIleValThr505560GluGluSerGlyValIleAspSerTyrGlyLysAsnTyrAspTyrIle65707580AlaIleValAspProLeuAspGlySerThrAsnPheValSerGlyIle859095ProTrpSerSerValSerIleAlaIleTyrAsnArgAspGluGluAsp100105110IleLeuSerSerAsnValGlyAlaValSerSerIlePheThrProTyr115120125ThrPheSerTyrAspGluGlySerAlaTyrValAsnGlyValLysIle130135140AlaGluIleLysLysProGluLysIleLeuLeuLeuAlaTyrPheSer145150155160ArgSerLysLeuProAsnLeuLysLeuPhePheGluLysIleGlyGln165170175GlyTyrLysIleArgSerLeuGlySerAlaSerLeuAspMetIleLeu180185190ValCysThrGlyArgAlaThrMetPhePheAspIleArgGlyLysLeu195200205ArgAsnValAspIleAlaAlaSerSerAsnPheCysSerArgLeuGly210215220ValIleProTyrAspIleGlyLeuArgLysIleLysSerSerLeuThr225230235240GluValSerValValLysAspLeuValIleSerLeuAspGluSerLeu245250255LeuArgSerPheSerLeuAlaLeuGlnThrVal260265<210>173<211>272<212>PRT<213>Thermanaerothrixdaxensis<400>173MetThrThrThrProIleValLeuSerIleAspLeuAspGlyThrLeu151015LeuIleGluGluArgGluValHisProGluAspValHisLeuLeuHis202530HisProLeuProValThrLeuIleLeuAlaThrGlyArgSerLeuGlu354045SerAlaArgArgValLeuGluArgAlaGlyLeuTyrArgHisGluPro505560LeuProPheProLeuValLeuGlnAsnGlyAlaAlaIleTyrArgPro65707580GlnGluValLeuGluAspTyrPheProIleProProGluAlaGluThr859095GlnLeuHisAlaLeuAlaProGlnPheSerGluAlaAlaLeuLeuTrp100105110GlnGlyLeuAspGlyAlaTyrLeuGluGluIleThrProPheGlyLeu115120125GlnAlaAlaGlyArgPheGlyPheLeuProArgProLeuSerGluAla130135140ProAlaGlyGlnGlyPheGlyLysLeuMetCysLeuSerAspArgPro145150155160GluValLeuLysAlaPheAlaAlaAlaThrArgHisLeuProValGlu165170175GlySerTyrSerLeuAspValIleTyrGluValThrArgGlnGlyVal180185190HisLysGlyPheGlyLeuArgIleLeuLeuGlnArgLeuGlyLeuThr195200205HisAlaArgLeuValAlaValGlyAspGlyAspAsnAspValProMet210215220PheSerCysAlaAspLeuAlaIleAlaProLeuAsnAlaSerProLeu225230235240AlaArgSerHisAlaHisArgLeuIleAspArgArgProHisGlyLeu245250255LeuThrProValLeuAspLeuLeuArgGlnGluGlyTrpLeuAspPro260265270<210>174<211>215<212>PRT<213>嗜热厌氧菌(Thermoanaerobactersp.)<400>174MetAsnValLysAlaValValPheAspLeuAspGlyThrLeuIleAsp151015SerLysLysAspIleAlaMetAlaAlaAsnLysThrLeuSerGluLeu202530GlyLeuHisThrLeuProGluAspThrIleAlaSerPheIleGlyTyr354045GlyGlyGluLeuPheIleLysArgCysIleGlyGluLysAsnIleAsp505560LysPheGluGluAlaPheLysLysPheLysGluAsnTyrSerGluLeu65707580CysIleIleHisThrSerLeuPheProGlyValGluGluValLeuGlu859095PheLeuLysLysArgLysIleAsnIleAlaLeuAlaThrAsnLysMet100105110IleSerLeuSerLysLysIleLeuLysHisLeuGluValGluLysTyr115120125PheSerIleMetLeuGlyProGluAspValThrAsnArgLysProHis130135140ProGluIleIleGluIleLeuLeuGlnAsnLeuAsnValArgProGlu145150155160GluThrLeuTyrValGlyAspSerGluIleAspValPheCysGlyLys165170175SerAlaGlyValTyrThrCysAlaValThrTyrGlyIleGlyAspIle180185190LysSerIleIleAlaAlaAspProAspPheIleIleThrAspLeuThr195200205LysLeuIleIleLeuLeuSer210215<210>175<211>273<212>PRT<213>热硫化氢嗜热厌氧杆菌(Thermoanaerobacterthermohydrosulfuricus)<400>175MetTyrLysLeuPheValThrAspAlaAspGlySerLeuLeuAsnSer151015AsnSerGluIleSerAspLysAsnLysGluAlaIleLysGluValIle202530SerArgGlyValIlePheThrIleAlaThrGlyArgMetPheSerSer354045IleLeuProTyrAlaPheGluLeuAsnValAsnAlaProValIleSer505560TyrAsnGlyAlaLeuIleLysAspIleTyrThrLysLysValTyrTyr65707580TyrAsnProIleGlnThrGluAspAlaIlePheAlaIleArgLeuLeu859095LysGluSerGlyTyrHisIleAsnLeuTyrIleAspAspGluLeuTyr100105110ValGluGluIleThrAspArgValGluTrpTyrLeuSerPheAsnAsn115120125ValThrValAsnAlaValGlyAsnLeuGluGluPheLeuLysArgThr130135140GlyGlyValThrAlaLysIleTyrAlaIleAsnAspMetLysAsnPro145150155160IleSerIleAspAlaGluIleTyrAspGluIleSerLysArgLeuThr165170175IleSerThrSerGlyGlyGlyHisLeuGluIleAsnAlaLysGlyVal180185190SerLysGlyAsnAlaLeuLysThrLeuAlaAsnMetTyrSerIleLys195200205ArgGluGlnValValAlaIleGlyAspAsnLeuAsnAspLeuSerMet210215220IleGluTyrAlaGlyLeuGlyValAlaMetGlyAsnAlaProAspIle225230235240ValLysIleLysAlaAspTyrThrThrLeuSerAsnAspGluAspGly245250255ValAlaHisValIleAspLysPhePheLeuAsnLysLysThrValAla260265270Val<210>176<211>273<212>PRT<213>热硫化氢嗜热厌氧杆菌(Thermoanaerobacterthermohydrosulfuricus)<400>176MetTyrLysLeuPheValThrAspAlaAspGlySerLeuLeuAsnSer151015AsnSerGluIleSerAspLysAsnLysGluGluIleLysGluValIle202530SerArgGlyValIlePheThrIleAlaThrGlyArgMetPheSerSer354045IleLeuProTyrAlaPheGluLeuAsnValAsnAlaProValIleSer505560TyrAsnGlyAlaLeuIleLysAspIleTyrThrLysLysValTyrTyr65707580TyrAsnProIleGlnThrGluAspAlaIlePheAlaIleArgLeuLeu859095LysGluSerGlyTyrHisIleAsnLeuTyrIleAspAspGluLeuTyr100105110ValGluGluIleThrAspArgValGluTrpTyrLeuSerPheAsnAsn115120125ValThrValAsnAlaValGlyAsnLeuGluGluPheLeuLysArgThr130135140GlyGlyValThrAlaLysIleTyrAlaIleAsnAspMetLysAsnPro145150155160IleSerIleAspAlaGluIleTyrAspGluIleSerLysArgLeuThr165170175IleSerThrSerGlyGlyGlyHisLeuGluIleAsnAlaLysGlyVal180185190SerLysGlyAsnAlaLeuLysThrLeuAlaAsnMetTyrSerIleLys195200205ArgGluGlnValValAlaIleGlyAspAsnLeuAsnAspLeuSerMet210215220IleGluTyrAlaGlyLeuGlyValAlaMetGlyAsnAlaProAspIle225230235240ValLysIleLysAlaAspTyrThrThrLeuSerAsnAspGluAspGly245250255ValAlaHisValIleAspLysPhePheLeuAsnLysLysThrValAla260265270Val<210>177<211>257<212>PRT<213>威吉利热厌氧杆菌(Thermoanaerobacterwiegelii)<400>177MetLysAspGluLysGlyIleValValAspIleIleLysLysAlaGly151015GluIleLeuGlnAspGlyTrpAsnLysLysAsnPheLysIleTyrArg202530LysGlyThrIleAsnLeuValThrGluIleAspLysLysIleGluPhe354045LeuIleIleGlnLeuLeuLysGlnTyrPheProAspTyrGlyIleLeu505560ThrGluGluSerLysGluIleAsnSerLysAlaAsnValArgTrpIle65707580IleAspProLeuAspGlyThrThrAsnTyrIleLysGlnTyrProPhe859095ValAlaIleSerIleAlaLeuGluLysGluGlyGluLeuIleLeuGly100105110ValValTyrAsnProIleLeuAsnGluMetPheIleAlaGlnLysGly115120125CysGlyAlaThrTyrAsnGlyLysSerIleHisValSerLysIleLys130135140GluLeuGlySerAlaValLeuAlaSerGlyPheProTyrAspAlaTrp145150155160GluAsnProAspAsnAsnAlaLysGlnTrpArgGlnPheLeuThrArg165170175SerLeuSerLeuArgCysAspGlySerAlaAlaLeuAspLeuCysArg180185190ValAlaCysGlyGlnLeuAspGlyTyrTrpGluLysGlyIleSerPro195200205TrpAspValAlaAlaGlyIleValIleLeuArgGluAlaGlyGlyIle210215220IleThrAspTyrLeuGlyGluGluAsnPhePheLysArgGlyGluVal225230235240ValAlaAlaAsnProValLeuHisAlaGlnMetLeuLysValLeuAsn245250255Asn<210>178<211>215<212>PRT<213>威吉利热厌氧杆菌(Thermoanaerobacterwiegelii)<400>178MetAsnValLysThrIleIlePheAspLeuAspGlyThrLeuIleAsp151015SerLysLysAspIleValMetAlaIleAsnLysThrLeuArgAspLeu202530AspIleProThrLeuProGluAspAspIleIleProPheMetSerTyr354045GlyProGluValPheIleLysGlnCysIleGlyGluLysAsnAlaAsp505560LysPheGluArgAlaPheGluLysPheLysGluAsnTyrSerGluArg65707580CysIleValTyrThrSerLeuPheProGlyValArgGluValLeuGlu859095PheLeuLysGluArgLysIleAsnIleAlaLeuAlaThrAsnLysMet100105110MetSerLeuSerLysLysIleLeuGlnHisPheGlyLeuGluLysTyr115120125PheSerIleMetLeuGlyProGluAspValThrAsnLysLysProHis130135140ProGluIleIleGluIleIleLeuGlnLysLeuAsnValLysArgGlu145150155160GluAlaLeuTyrValGlyAspSerGluIleAspValLeuCysGlyLys165170175SerAlaGlyValTyrThrCysAlaValThrTyrGlyIleGlyAspIle180185190LysSerIleIleAlaAlaAsnProAspPheIleIleThrAspLeuThr195200205LysLeuIleLeuLeuValSer210215<210>179<211>205<212>PRT<213>木聚糖热厌氧杆菌(Thermoanaerobacteriumxylanolyticum)<400>179MetSerThrArgLeuPheIleValArgHisGlyGluThrLeuTrpAsn151015ArgGlnLysLysIleGlnGlyAlaSerAspThrGlnLeuSerAspGlu202530GlyMetLysGlnAlaTyrLeuLeuSerGlnArgLeuLysAsnGluIle354045IleAspValIlePheSerSerAspLeuAspArgAlaTyrLysThrAla505560ThrPheIleAlaLysAsnPheAsnLeuAspValIleLysLeuProGlu65707580LeuArgGluIleSerPheGlyValTrpGluGlyLeuThrValAspGlu859095IleGluLysSerTyrLysGluLeuTyrHisThrTrpLysThrAsnPro100105110ProGluAlaThrIleGluGlyAlaGluThrLeuLysAlaValGlnAsp115120125ArgIleLeuAsnAlaThrAsnLysIleIleGluGlnTyrLysAsnLys130135140AsnIleLeuIleValSerHisGlyThrThrIleLysAlaLeuIleLeu145150155160GlyMetLeuAsnLeuAspLeuSerPheTyrProLysIleArgGlnAsp165170175AsnThrAlaLeuAsnIleIleAspValLysAspAspGlyAsnCysVal180185190LeuValLeuLeuAsnAspThrCysHisLeuArgGluArg195200205<210>180<211>595<212>PRT<213>耐盐嗜热双岐菌(Thermobifidahalotolerans)<400>180MetArgTyrHisValLeuAlaCysAspTyrAspGluThrIleAlaHis151015GlyGlyArgValAspAspAlaThrValThrAlaLeuGluHisLeuSer202530ArgSerGlyArgArgLeuValMetValThrGlyArgArgLeuAspAsp354045LeuPheGlyValPheAspHisValAspLeuPheAspMetValValAla505560GluAsnGlyGlyValLeuTyrAspProGlyArgArgAspArgArgPro65707580LeuAlaGluArgProProAlaGluPheValGluArgLeuArgArgHis859095GlyValSerProLeuAlaValGlyGluValValValAlaThrArgGlu100105110ProHisAspArgThrValLeuAspAlaIleArgGluLeuGlyLeuGlu115120125LeuGlnValIleTyrAsnLysGlyAlaValMetValLeuProProGly130135140ValAsnLysAlaSerGlyLeuAlaAlaAlaLeuArgArgLeuGluPhe145150155160SerProHisSerThrValAlaValGlyAspAlaGluAsnAspHisAla165170175MetLeuHisMetCysGluCysAlaValAlaValAlaGlyAlaLeuGlu180185190AlaValLysArgThrSerAspLeuValLeuGluArgProAspGlyGlu195200205GlyValAlaGluLeuValAspArgLeuLeuAlaThrAspLeuAlaGlu210215220ValProValSerArgHisAspLeuHisPheGlyThrAlaGlyAlaAla225230235240ProThrAlaIleAlaProHisGlyArgGlyGlyAlaValAlaGlyPro245250255SerGlySerGlyLysSerThrAlaAlaThrAlaLeuLeuGluArgLeu260265270GlyGluSerGlyTyrGlnTyrCysValIleAspProGluGlyAspTyr275280285AlaAspPheGluGlyValThrAlaPheGlyAspAlaThrArgAlaPro290295300SerIleAspGluValLeuThrAlaLeuArgAspProAspHisAsnVal305310315320AlaValAsnMetLeuAlaValProLeuArgAspArgProAlaPhePhe325330335AlaGlyLeuLeuProArgLeuValGlyLeuArgAlaGluLeuGlyHis340345350ProAlaTrpLeuValValAspGluAlaHisHisLeuMetProValGlu355360365LeuAlaGlnLeuProLeuArgValProAlaAspValGlyGlyLeuMet370375380LeuValThrValHisProGluThrLeuSerProLeuValLeuArgLeu385390395400ValAspThrValValAlaValGlyAspAspProGlyGlnThrLeuAsp405410415AlaPheAlaAlaAlaThrGlyHisArgProProAlaLeuGluProAla420425430ProAlaGluProAspArgGlnGlnGluSerValValSerAspIleAla435440445ValTrpArgValAlaGluThrAlaAlaGlnArgValLysLeuAlaPro450455460GlyGluThrHisArgThrArgHisArgArgLysTyrAlaAlaGlyThr465470475480LeuAlaProAspLysSerPheTyrPheThrGlyProGlnGlyArgLeu485490495ArgLeuArgAlaArgAsnLeuHisSerPheValGluLeuAlaGluGly500505510ValAspAspAspThrTrpThrHisHisLeuArgArgHisAspTyrSer515520525ArgTrpLeuArgGlySerValArgAspGlyGluLeuAlaAspLeuVal530535540ArgArgValGluGlnAspThrGlyArgAlaProSerAspSerArgArg545550555560GluValAlaArgLeuIleAspGluArgTyrThrLeuProAlaGluPro565570575ThrSerTyrAspProAspArgGlyArgGluThrGlyProSerGlyArg580585590ArgArgPro595<210>181<211>237<212>PRT<213>耐盐嗜热双岐菌(Thermobifidahalotolerans)<400>181MetGlyLeuArgGluThrAspThrGluPheValProProArgAlaAla151015LeuPheAspLeuAspGlyThrLeuIleAsnSerGluProArgSerAla202530AlaValTrpAlaArgLeuLeuArgAlaArgGlyIleThrProAspGlu354045AlaLeuLeuArgArgPheMetGlyArgArgGlyGlyAspValValAla505560GluLeuProHisLeuPheProGlyGluSerLeuGluGlnIlePheAsp65707580GluLeuTrpArgHisGlyGlnAspProAspLeuProGlnValAlaPro859095LeuProGluSerValAlaPheLeuHisHisLeuHisSerGlnGlyVal100105110ProPheAlaLeuValThrSerAlaGlyArgGluTrpAlaGluSerAla115120125LeuGluTrpLeuGlyValArgGlyMetPheArgGlyLeuValThrAla130135140GlyAspValThrValGlyLysProHisProGlnGlyTyrLeuGlyGly145150155160AlaGluIleLeuGlyHisGluProGluHisIleValValPheGluAsp165170175ThrProAlaGlyIleMetAlaGlyArgGlyAlaGlyMetArgValVal180185190GlyIleThrThrThrHisProProGluAlaLeuThrGlnAlaAspLeu195200205ValValGluHisLeuThrGlnValAlaTrpProArgLeuSerLeuArg210215220AspProGluProProArgSerSerPheAlaGlyArgGly225230235<210>182<211>236<212>PRT<213>速生热球菌(Thermococcusceler)<400>182MetGlyArgValLysAlaIleSerLeuAspIleAspGlyThrIleThr151015TyrProAspArgArgLeuHisGluGluAlaMetArgAlaIleArgLeu202530AlaGluSerLeuGlyValProValMetLeuValThrGlyAsnSerVal354045ProPheAlaGluAlaMetAlaIleMetIleGlyThrSerGlyProVal505560ValAlaGluAspGlyGlyAlaLeuSerIleLysAspGlyArgLeuArg65707580LysArgValTyrLeuThrThrMetAspGluGluTrpIleLeuTrpSer859095GluValLysLysArgTyrProGlyAlaIleLeuSerPheSerMetPro100105110GluArgLysAlaGlyLeuValIleLeuArgThrValProValGluAla115120125ValArgGluLeuIleLysGluLeuGlyLeuAsnLeuIleAlaValAsp130135140SerGlyPheAlaIleHisValLysLysProTrpIleAsnLysGlyThr145150155160GlyIleGluLysAlaCysGluValLeuGlyIleLysProArgGluVal165170175AlaHisValGlyAspGlyGluAsnAspLeuAspAlaPheArgValVal180185190GlyTyrArgValAlaValGlyGlnAlaProGluSerLeuLysGlyGlu195200205AlaAspTyrValThrGluLysThrTyrGlyAlaGlyGlyAlaGluGly210215220IleLeuHisValLeuArgGluPheGlyTyrMetGlu225230235<210>183<211>236<212>PRT<213>速生热球菌(Thermococcusceler)<400>183MetGlyArgValLysAlaIleSerLeuAspIleAspGlyThrIleThr151015TyrProAspArgArgLeuHisGluGluAlaMetArgAlaIleArgLeu202530AlaGluSerLeuGlyValProValMetLeuValThrGlyAsnSerVal354045ProPheAlaGluAlaMetAlaIleMetIleGlyThrSerGlyProVal505560ValAlaGluAspGlyGlyAlaLeuSerIleLysAspGlyArgLeuArg65707580LysArgValTyrLeuThrThrMetAspGluGluTrpIleLeuTrpSer859095GluValLysLysArgTyrProGlyAlaIleLeuSerPheSerMetPro100105110GluArgLysAlaGlyLeuValIleLeuArgThrValProValGluAla115120125ValArgGluLeuIleLysGluLeuGlyLeuAsnLeuIleAlaValAsp130135140SerGlyPheAlaIleHisValLysLysProTrpIleAsnLysGlyThr145150155160GlyIleGluLysAlaCysGluValLeuGlyIleLysProArgGluVal165170175AlaHisValGlyAspGlyGluAsnAspLeuAspAlaPheArgValVal180185190GlyTyrArgValAlaValGlyGlnAlaProGluSerLeuLysGlyGlu195200205AlaAspTyrValThrGluLysThrTyrGlyAlaGlyGlyAlaGluGly210215220IleLeuHisValLeuArgGluPheGlyTyrMetGlu225230235<210>184<211>251<212>PRT<213>Thermococcuslitoralis<400>184MetTyrGluTrpAsnGluIleAlaLeuAsnLeuAlaLysAspIleGlu151015ArgGluValMetProLeuPheGlyThrLysLysAlaGlyGluPheIle202530GlyPheSerProSerGlyAspLysThrLysLeuValAspLysValAla354045GluAspValValLeuGluTyrLeuArgProLeuGlyValAsnValVal505560SerGluGluIleGlyAsnIleGluAlaGlySerGluTyrThrIleVal65707580ValAspProIleAspGlySerPheAsnPheIleGlnGlyIleProIle859095PheGlyPheSerPheAlaValPheLysAsnGluLysProValTyrAla100105110MetIleTyrGluPheIleThrLysAsnValTyrGluGlyIleProGly115120125GluGlyAlaTyrLeuAsnGlyGluArgIleArgValArgHisLeuAsn130135140GluLysSerIleSerIleSerPheTyrThrArgGlyArgGlyAlaArg145150155160LeuValGluLysValLysArgThrArgValLeuGlyAlaIleAlaVal165170175GluLeuAlaTyrLeuAlaArgGlySerLeuAspGlyValIleAspIle180185190ArgAsnTyrValArgProThrAspIleAlaAlaGlyTyrIleIleAla195200205LysGluAlaGlyAlaIleIleThrAspAspSerGlyGluGluIleLys210215220PheArgLeuAspAlaArgGluLysLeuAsnIleIleAlaValAsnAsp225230235240LysArgLeuLeuLysLeuIleLeuGluValIle245250<210>185<211>237<212>PRT<213>深嗜热球菌(Thermococcusprofundus)<400>185MetLysValArgAlaIleSerLeuAspIleAspGlyThrIleThrTyr151015ArgAspArgArgLeuSerIleGluAlaLeuLysAlaIleArgLeuAla202530GluSerLeuGlyValProValMetLeuValThrGlyAsnSerValPro354045PheAlaGluAlaAlaAlaIlePheIleGlyThrSerGlyProValIle505560AlaGluAspGlyGlyAlaLeuSerLeuLysGlyLysGlyThrMetArg65707580LysArgValPheLeuThrAspMetAspGluGluTrpIleLeuTrpSer859095GluLeuLysLysArgTyrProLysAlaGluLeuSerPheSerThrMet100105110GluArgLysAlaGlyLeuValIleArgArgThrIleProValGluAla115120125ValArgGluIleIleArgGluLeuGlyLeuAsnLeuValAlaValAsp130135140SerGlyPheAlaIleHisValLysLysProTrpIleAsnLysGlyThr145150155160GlyIleGluLysAlaCysGluTyrLeuGlyIleSerProLysGluVal165170175AlaHisValGlyAspGlyGluAsnAspLeuAspAlaPheArgValVal180185190GlyTyrArgValAlaIleAlaGlnAlaProGluSerValLysGluLys195200205AlaAspTyrValThrGlnLysProTyrGlyAspGlyGlyAlaGluAla210215220ValMetHisIleLeuArgLysPheGlyTyrLeuGluGlu225230235<210>186<211>215<212>PRT<213>密涅瓦热发状菌(Thermocrinisminerva)<400>186MetPheLysLeuValIlePheAspLeuAspGlyThrLeuIleAspSer151015CysGlnAspIleThrLeuCysLeuAsnMetThrLeuArgAspPheGly202530LysProGlnValGluSerGlnThrValArgArgTyrIleGlySerGly354045AlaArgAlaLeuLeuGluLysPhePheProProGluAspValAspLys505560AlaLeuGluValPheArgGlyTyrTyrArgGlnHisProValValTyr65707580ThrArgProTyrGluGlyIleProGluAlaLeuGluPheLeuArgSer859095LysGlyValIleMetThrValValThrAsnLysMetGluGluIleSer100105110TyrLysIleLeuAspThrLeuLysLeuLeuAspTyrPheSerLeuVal115120125ValGlyGlyAspThrTyrHisGluLysLysProSerAlaLeuProIle130135140IleLysThrLeuGluLysPheSerValLysProLysGluSerLeuIle145150155160ValGlyAspThrGluAlaAspIleLeuSerGlyLysArgAlaSerVal165170175TyrThrAlaLeuAlaLeuTrpGlyTyrThrLysAspMetGlnGluGlu180185190ProAspPheIleLeuAsnThrProThrGluIleIleGluLeuTrpAsn195200205SerArgSerThrSerArgVal210215<210>187<211>404<212>PRT<213>Thermocrinisruber<400>187MetGluGlyGlySerPheLeuAsnLeuIleGluSerLeuAspLysPhe151015AlaValGlyIlePheArgGlnPheLeuIleArgGluAspThrAspLys202530IleLeuIleLeuThrProAsnGluGluTyrLysArgTrpAlaIleAla354045TyrLeuSerThrArgLeuLysGlyPheGlyLysLysValValValGlu505560CysGluGlyGluArgLysGluGlnLysGluIleGluSerProLysAsp65707580AsnLeuLeuAspLysTyrThrPheGluAsnPheValValGlyProSer859095AsnGluLeuAlaTyrLysValCysLeuGluValThrLysAsnProGly100105110LysLeuPheAsnProLeuPheIleTyrGlyArgSerGlyLeuGlyLys115120125ThrHisLeuLeuHisAlaIleGlyAsnGlnLeuLysAlaArgTyrArg130135140ValLeuTyrIleProLeuMetAspPheSerAspSerMetValLysAla145150155160PheLysGluAsnArgValGluGluPheArgGluArgPhePheAsnLeu165170175AspValLeuLeuLeuAspAspValGlnPheLeuValGlyLysGluArg180185190ThrGlnIleGluLeuPheArgIleTyrGluLysLeuGlnAlaGluGlu195200205LysGlnIleValLeuValSerAspArgHisProArgAspLeuLysAsp210215220ValSerGluArgLeuValSerArgPheGluSerGlyLeuIleIleGlu225230235240IleGlyLeuAspGluGluThrLysArgArgIleIleLysGlnLysLeu245250255IleLeuTyrGlyLeuProLeuAspGlnGluThrIleAspTyrValPhe260265270GluAsnThrGlyTyrAsnValArgGluIleGluGlyPheValLysThr275280285LeuLysValSerGlyIleLysArgLeuProLysSerGluGluLeuAsp290295300LysGluLysLysValGlnLeuIleIleAsnThrValAlaLysGlyPhe305310315320LysLeuAsnProGluLeuLeuLysLysAspThrLysGluArgArgMet325330335IleAsnAlaArgHisIleAlaMetPheLeuCysLysThrIleLeuAsn340345350LeuProTyrSerGlnIleGlyGluPhePheGlyLysLysAspHisThr355360365AlaValMetTyrAlaValLysLysValGluGlnArgCysArgGluAsp370375380ArgLysPheMetTyrMetValSerPhePheGluLysSerIleArgLys385390395400SerLeuGlyLeu<210>188<211>222<212>PRT<213>Thermoflexushugenholtzii<400>188MetSerArgGlyProAlaPheLeuPheAspLeuAspGlyThrLeuVal151015AspSerValTyrGlnHisValLeuAlaTrpArgGluAlaLeuGluAla202530ValGlyIleSerLeuSerValTrpArgIleHisArgArgIleGlyMet354045SerGlyGlyLeuLeuLeuArgAlaLeuLeuArgGluThrGlyArgGlu505560ValThrSerGluGluValGlnArgIleGlnGlnGlnHisAlaGluAla65707580PheAlaArgLeuLeuProGlnValArgProLeuProGlyAlaArgGlu859095LeuLeuGluAlaLeuThrArgAlaGlyIleProTrpAlaIleAlaThr100105110SerGlyArgArgAspValAlaArgProLeuLeuGluLeuLeuGlyVal115120125AspGluGlyIleProValIleThrArgGluAspValProTyrAlaLys130135140ProAspProAspLeuPheLeuAlaAlaAlaAlaArgLeuGlyIlePro145150155160IleAlaGluCysIleValValGlyAspSerValTrpAspLeuLeuAla165170175AlaArgArgAlaGlyAlaLeuSerValGlyLeuLeuSerGlyGlyTyr180185190GlyArgGluGluLeuGluArgAlaGlyAlaTyrArgValTyrGluAsp195200205ProAlaAspLeuLeuArgHisLeuAspGluValGlyValArg210215220<210>189<211>255<212>PRT<213>莱廷格热袍菌(Thermotogalettingae)<400>189MetAspArgMetAspPheSerIleLysIleAlaArgLysValGlyLeu151015TyrLeuMetGluHisTrpGlyAsnAlaGluAsnValArgGlnLysSer202530SerPheGlnAspLeuValSerAspCysAspLysGlnAlaGlnLysMet354045IleValGlnLysIleLysAspHisPheProAspAspAlaIleLeuAla505560GluGluGlyLeuPheGluLysGlyAspArgMetTrpIleIleAspPro65707580IleAspGlyThrMetAsnTyrValHisGlyLeuProSerPheAlaIle859095GlyIleAlaTyrValGluLysGluGlnValIleLeuGlyValAlaHis100105110AspProValLeuAsnGluThrTyrTyrAlaIleLysGlyGlnGlyAla115120125TyrLysAsnGlyGluArgIleAsnValSerGluAsnSerLeuLeuLys130135140AspSerIleGlyAsnThrGlyPheTyrThrAspPheThrGlyIlePhe145150155160IleSerAlaIleGluLysLysValArgArgValArgMetThrGlySer165170175AlaIleLeuAlaGlyAlaTyrValAlaCysGlyArgPheAspPhePhe180185190IleAlaLysArgAlaAsnSerTrpAspValAlaProLeuPheValLeu195200205ValProGluAlaGlyGlyIleValThrAspLeuSerGlyAsnGlnAla210215220HisLeuAsnThrGlyAsnPheLeuPheSerAsnGlyLeuLeuHisAsp225230235240GlnValLeuGluValIleArgGluValAsnLysLysValArgLys245250255<210>190<211>201<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>190MetArgLeuTyrLeuIleArgHisGlyGluThrIleTrpAsnGluLys151015GlyLeuTrpGlnGlyIleAlaAspValProLeuAsnGluLysGlyLys202530AspGlnAlaLysLysLeuAlaGluArgLeuLysArgValAspAlaIle354045TyrSerSerProLeuLysArgCysLeuGluThrAlaArgGluIleAla505560GluArgPheLysLysGluValIleValGluGluAspLeuArgGluCys65707580GluIleSerLeuTrpAsnGlyLeuThrValGluGluAlaMetArgGlu859095TyrProGluGluPheLysArgTrpSerThrAspProAsnPheGlyThr100105110LysGlyLeuGluSerMetLysSerValGlnAspArgMetValLysVal115120125MetMetLysIleValSerGlnGluArgLeuAsnGlySerGluAspVal130135140ValIleValSerHisSerLeuSerLeuArgSerPheIleCysTrpVal145150155160LeuGlyLeuProLeuArgLeuHisArgAsnPheLysLeuAspAsnAla165170175SerLeuSerIleValAspValGluSerArgProArgLeuValLeuLeu180185190AsnAspThrCysHisLeuGluAspThr195200<210>191<211>268<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>191MetTyrArgValPheValPheAspLeuAspGlyThrLeuLeuAsnAsp151015SerLeuGluIleSerGluLysAspArgLysValIleGluArgLeuSer202530ArgAsnCysHisValValPheAlaSerGlyArgMetLeuValSerThr354045LeuAsnValGluLysArgTyrPheLysArgThrPheProThrIleAla505560TyrAsnGlyAlaMetValTyrLeuProGluGluGlyValValLeuAsn65707580GluLysIleProProGluValAlaLysAspIleIleGluTyrIleLys859095ProLeuAsnValHisTrpGlnAlaTyrIleAspAspValLeuTyrSer100105110GluLysAspAsnGluGluIleLysSerTyrAlaArgHisSerAsnVal115120125AspTyrArgValGluProAsnLeuSerGluLeuValSerLysMetGly130135140ThrThrLysLeuLeuLeuIleAspThrProGluArgLeuAspGluLeu145150155160LysGluIleLeuSerGluArgPheLysAspValValLysValPheLys165170175SerPheProThrTyrLeuGluIleValProLysAsnValAspLysGly180185190LysAlaLeuLysPheLeuArgGlyArgMetAsnTrpLysLysGluGlu195200205IleValValPheGlyAspAsnGluAsnAspLeuPheMetPheGluGlu210215220AlaGlyLeuArgValAlaMetGlyAsnAlaIleAspLysValLysGlu225230235240AlaAlaAspValValThrLeuThrAsnAsnAspSerGlyValSerTyr245250255ValLeuGluArgIleSerThrAspCysLeuAspGlu260265<210>192<211>201<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>192MetIleArgAsnIleValPheAspLeuGlyGlyValLeuIleAspTrp151015ArgProCysGluTyrLeuValGluSerPheProGluAspValAlaLys202530ValLeuGluArgGluIlePheLysHisGluAspTrpLysLysMetAsp354045ArgGlyThrLeuProGluAsnAspLeuTrpGluLysLysLysLysGlu505560LeuSerGluTyrArgGluTyrValGluLysLeuGluArgGluValPro65707580LysLeuLeuLysProIleGluGluAsnValLysLeuLeuSerIleLeu859095LysGluLysAsnPheLysLeuTyrValLeuSerAsnTyrGlyLysIle100105110TyrPheGluMetValArgArgArgTyrArgPhePheAspPhePheAsp115120125GlyMetValIleSerSerHisValGlyPheIleLysProGluLysGlu130135140IleTyrLeuGluLeuIleArgLysTyrLysIleThrProLysGluSer145150155160LeuPheIleAspAspMetGluGluAsnValLysAlaAlaGluGluLeu165170175GlyPheAsnThrIleHisLeuProGluProSerArgLeuLysGluLeu180185190LeuPheGluThrLeuLysIleGlyArg195200<210>193<211>564<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>193MetLeuLeuArgIleIleProSerAspArgIleSerSerThrGluVal151015LysThrLeuGluArgValTyrValValGlyHisArgAsnProAspThr202530AspSerValCysSerAlaIleGlyTyrAlaHisTyrLysAlaGluVal354045GluLysLysLysGlnPheIleProAlaArgCysGlyGluLeuThrAsn505560GluAlaLeuPheValLeuAspTyrPheLysValArgProProIlePhe65707580LeuGluThrLeuGluProThrValGluAspLeuGluLeuLysAspPro859095IlePheValSerProAspThrProValTyrAspValAlaMetLeuMet100105110GluGlyLysGlyIleLysAsnValProValValSerArgGlyLysMet115120125IleGlyValValThrGluSerAsnLeuAlaArgValTyrValArgArg130135140LeuLysIleGluProLeuValIleHisProValProLeuGluGlnLeu145150155160ValArgValLeuLysAlaGluValValCysAspHisLeuLysGluLys165170175ThrIleSerGlyLysValHisIleAlaValAspAlaLeuHisValLeu180185190LeuGlyLysIleGluIleGlyAspValValIleValGlyAspAsnGlu195200205ProSerGlnIleAlaLeuLeuGluLysGlyAlaLysLeuMetIleIle210215220ValAsnAsnAlaProValSerSerArgValLeuGluIleAlaLysGlu225230235240LysGlyAlaAlaValLeuArgValLysPheAspAlaPheGlyAlaAla245250255LysLeuIleAsnLeuAlaLeuProValThrLeuValMetSerLysLys260265270PheProThrValThrLysArgAspThrLeuGluAspValLysAsnIle275280285ValPheThrSerLysLeuArgAlaAlaPheValGluAspGluLysGly290295300LysLeuLeuGlyValIleThrArgThrAspLeuMetLysAspValArg305310315320LysLysValIleLeuValAspHisAsnGluIleThrGlnAlaProGlu325330335GlyValGluLysAlaGluIleLeuGluIleIleAspHisHisArgLeu340345350GlyGlyLeuSerThrLeuAsnProValPhePheTyrAsnGluProVal355360365GlySerThrSerThrIleValThrGluPhePheLeuArgAspGlyVal370375380LysMetGluArgGluIleAlaGlyValLeuLeuAlaGlyIleValSer385390395400AspThrLeuPhePheLysLeuSerThrThrThrGluLysAspArgAla405410415MetAlaGlyPheLeuAlaArgIleThrLysLeuAspLeuGluLysPhe420425430AlaLysLysValLeuLysGluGlyMetLysIleProGluAsnValAsp435440445ProLeuAspLeuLeuLysArgAspValLysValTyrGluMetGlyGlu450455460GluSerPheAlaValSerGlnIleMetThrSerAspPheSerValLeu465470475480LeuLysGluLysGluLysPheAlaThrAlaLeuLysSerLeuLysGly485490495GluLeuGlyValThrHisCysPheValLeuPheThrAsnProIleGlu500505510GluAlaSerLeuValMetValGluGlyAspGlnArgIleLeuGluLys515520525AlaPheAspGlyAlaGluLysLysAspGlyLeuPheLeuLeuLysGly530535540ValMetSerArgLysLysAspPheValProArgIleGlyAspIleLeu545550555560ArgArgGluArg<210>194<211>136<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>194MetAspThrPheLeuTyrHisIleLeuAlaIleAspValLysGlyTyr151015ValAspProSerLeuProValGluGluIleValLysArgLeuLysGlu202530GlnAsnAlaLeuValIleAlaAlaHisProAspArgLysLysGlnAsp354045GluGluHisLeuSerTrpPheLeuTrpValAsnMetGluArgPheLys505560AspMetPheAspAlaTrpGluValAlaAsnArgAspAspLeuPheAsn65707580SerIleGlyValLysLysTyrArgTyrValAlaAsnSerAspPheHis859095GluValTrpHisValTyrSerTrpLysThrLeuIleArgSerGluArg100105110AsnValGluAlaIleLysGluAlaIleArgLysAsnThrAspValAla115120125IleTyrLeuMetArgGluLysThr130135<210>195<211>443<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>195MetAsnGluArgAspIleCysArgArgIleSerGluIleLeuGlyIle151015AspTyrAspCysThrArgGlyIleAspGluLeuLeuLysIleLeuGlu202530TyrGluIleAsnGluTyrLysLysSerLeuGluGluGlnThrIlePhe354045MetGluAlaGlnLeuGluGluLeuSerArgSerTyrGluGluIleSer505560ThrLeuLeuGluIleSerGluIlePheGlyGlyPheGluPheProMet65707580AsnLeuArgGluLysLeuGluLysValIleProLeuLeuLysLysVal859095ValLysPheLysAspTyrIleValArgValGluAspLeuSerIleGly100105110AsnMetSerHisGluGluValGluArgGluIleGluGluArgGluLys115120125ThrValLeuIleGluProGlyGluSerLysLysPheSerAsnLeuLeu130135140PheValProIleIleGlyAsnArgTyrTyrGlyTyrMetCysPheSer145150155160GlyLysGluGluGlyIleValPheThrAlaSerAspArgArgIleThr165170175GluValThrSerArgTyrIleAlaAsnAlaLeuAspArgIleAspPhe180185190LeuGlnLysGluIleGluArgGlnArgLeuGluGluGlnMetGluIle195200205AlaArgArgIleGlnSerGluLeuLeuProArgGluLeuProArgThr210215220GlyPheIleAspIleThrAlaCysSerArgProAlaValGlnValGly225230235240GlyAspTyrTyrAspValPheAlaSerAspGlyLysValLeuAlaVal245250255MetGlyAspValAlaGlyLysSerValProAlaAlaLeuLeuMetSer260265270AlaValArgSerTyrLeuLysValLeuSerThrSerHisSerAspLeu275280285GluAspLeuValAsnHisLeuAsnSerIleLeuCysGluAspLeuSer290295300AsnAspArgPheValThrMetValPheLeuGluIlePheGlnAsnGly305310315320ThrLeuAsnLeuValAsnAlaGlyHisAsnProValTyrPheLeuHis325330335AspGlyGluIleValLysLeuGluAlaSerAlaIleProIleGlyIle340345350AlaGluTrpAsnTyrArgArgHisThrIleGlnLeuLysProAsnThr355360365PheIleValValTyrThrAspGlyIleThrGluAlaArgAsnThrLeu370375380GlyGluGluTyrGlyTyrGluArgLeuGluGluThrLeuArgGluTyr385390395400AsnGlyAspSerAlaGluAspLeuLeuSerLysLeuLysLysSerVal405410415GluGluPheSerLysAspValProGlnHisAspAspMetThrIleMet420425430ValLeuLysTyrLysGlyGlnGlnGluValGlu435440<210>196<211>266<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>196MetIleAlaLeuLeuAspMetGlySerAsnSerPheIleLeuLeuIle151015ValSerGluGluGlyGluValIleLeuGluGluValHisGluValGly202530IleAlaSerGlyAsnLeuGlnArgAlaLysGluValPheArgGluCys354045ValArgLysSerGluGluLeuGlyAlaAspLeuHisIlePheGlyThr505560AlaPhePheArgLysAsnProAspValPheTyrGluIleThrGlyGly65707580ArgGlyLysIleLeuSerGluGluGluGluAlaArgTyrSerTyrIle859095SerValValArgAspPheGlyLysGluAspIleLeuValAlaAspLeu100105110GlyGlyGlySerLeuGluLeuAlaTrpLysAspGlyTyrThrSerLeu115120125GluLeuGlyThrHisIleLeuAsnArgIlePheSerLeuThrLeuPro130135140PheArgLysSerValAspAspValValGluTyrValMetAspLysLeu145150155160ProAspLeuAlaLysSerAspLeuPheGlyValGlyGlySerPheVal165170175AlaLeuAlaAlaLeuMetLysGlyLysTrpAspLeuLysSerIleHis180185190GlySerThrLeuGluIleGluArgValGlnLysIleValAspGlnIle195200205ArgLysMetSerPheGluAspIleArgLysLeuLysIleLeuProGlu210215220GlyArgGluLysThrIleLeuAlaGlyGlyIleValThrIleAlaLeu225230235240LeuLysLysTyrSerProLysMetThrValSerThrLysGlyTyrArg245250255TyrGlyIleAlaTrpGluIleMetValGln260265<210>197<211>208<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>197MetGluLeuIleLysLeuLysLysGlyGlyValMetValGlyThrPhe151015ArgIleTyrValAlaThrThrAsnProHisLysValGluGluIleLys202530GluIleAlaProGluTrpAlaGluIleLeuProSerProGluLysIle354045GluValIleGluAspGlyGluThrPheLeuGluAsnSerValLysLys505560AlaIleValTyrGlyLysLysLeuLysSerProValIleAlaAspAsp65707580SerGlyLeuValIleTyrSerLeuGlyGlyPheProGlyValMetSer859095AlaArgPheMetGluGluTyrThrTyrGluGluLysMetLysThrIle100105110LeuLysMetLeuGluGlyLysAspArgLysAlaAlaPheValCysSer115120125AlaThrPhePheAspPheGlnArgAsnLeuLeuValSerValGluAsp130135140ArgValGluGlyTyrIleAlaGluGluIleArgGlyThrGlyGlyPhe145150155160GlyTyrAspProPhePheValProGluGlyTyrAspArgThrPheGly165170175GluMetProGluLeuLysLysLysLeuSerHisArgSerArgAlaPhe180185190ArgLysLeuPheSerIleLeuSerLysIleSerGluSerGluSerLeu195200205<210>198<211>436<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>198MetIleGlnValArgArgLeuIleValLeuValLeuAlaLeuLeuLeu151015ValAlaPheSerPheSerGlnValLysAsnIleIleTyrPheIleGly202530AspGlyMetGlyLeuSerGlnAlaTyrLeuThrSerLeuIleGluGly354045ArgProLeuSerPheMetLysThrProTyrThrGlyLeuValLysThr505560HisSerValAspSerTrpValThrAspSerAlaAlaAlaGlyThrAla65707580LeuAlaSerGlyPheLysThrLysAsnGlyMetIleAsnValLeuPro859095AspGlyThrArgValProThrIlePheGluIleAlaLysAlaTyrGly100105110AlaLysThrGlyIleValValThrCysArgValThrHisAlaThrPro115120125AlaAlaPheTyrAlaHisValLysSerArgSerGluGluAsnGluIle130135140AlaArgGlnLeuValGluSerGluThrValAspLeuValMetGlyGly145150155160GlyTrpAlaAsnPheLeuProGluSerLeuGlyGlyLysArgSerAsp165170175GlyLeuAsnLeuIleGluMetAlaLysLysLysGlyTyrValTyrVal180185190ThrLysLysGluAspLeuMetLysLeuProGluAsnThrGluLysVal195200205LeuAlaLeuPheAlaProSerHisLeuAspProAlaSerSerArgGlu210215220GluGlnProMetLeuTyrGluMetValLysLysAlaLeuGluIleLeu225230235240SerAsnAspGluGluProPhePheLeuMetValGluGlySerGlnIle245250255AspTrpGluAlaHisAspAsnAspIleTyrGlyValTrpLysGluVal260265270ValGluPheAspArgAlaValGlnValAlaLeuAspPheAlaLeuLys275280285ArgGlyAspThrLeuValIleValThrAlaAspHisGluThrGlyGly290295300LeuAlaLeuSerSerGlyAspTyrArgValAspValAspArgIleArg305310315320LysPheSerLysThrThrAspTrpIleLeuAlaAsnTyrSerLeuLys325330335AspArgGluSerPheLysLysAlaIleGluLysTyrPheGlyLeuThr340345350LeuSerAspSerAspLeuAspArgIleThrSerSerAsnAsnSerLys355360365ValGluLeuGlyArgValLeuSerGluLysValAsnValGlyTrpThr370375380ThrThrSerHisSerGlyValProValProIleTyrAlaPheGlyPro385390395400GlyAlaGluAsnPheThrGlyPheLeuAspAsnThrAspIleProArg405410415IleMetMetLysLeuAlaGlyTyrSerLeuGlnTyrProLeuValLys420425430GluProValLys435<210>199<211>174<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>199MetLysIleLeuIleThrGlyArgProGlyValGlyLysThrThrLeu151015IleLysLysIleSerSerLeuLeuGlnAsnAlaGlyGlyPheTyrThr202530GluGluMetArgGluLysGlyLysArgValGlyPheLysIleValThr354045LeuAspGlyArgGluGlyIleLeuAlaLysValGlyPheProSerGln505560HisArgValGlyLysTyrGlyValAsnLeuLysAspLeuGluGluLeu65707580GlyValAspSerValGluArgAlaLeuGluGluLysSerValValIle859095IleAspGluIleGlyLysMetGluLeuLeuSerGluArgPheLysArg100105110ValValGluLysAlaPheAsnSerGluLysAspLeuIleAlaThrIle115120125LysLysSerSerAspProPheValGluLysIleLysAsnLysLysAsp130135140ValIleIlePheGluLeuAsnGluArgAsnArgAspLeuLeuLeuLys145150155160ArgIleLeuAspMetLeuLysSerAsnArgGlyValGlyGlu165170<210>200<211>508<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>200MetArgArgTyrLeuValLeuPheLeuValLeuLeuLeuSerValPhe151015LeuLeuGlyValArgLeuThrIleLeuHisValAsnAspThrHisGly202530HisAlaTrpThrPheAspGluTyrGlnAsnProGlyIleGlyGlyLeu354045AlaAlaIleAlaThrIleValGluGluValLysArgGluValGluGlu505560GlnGlyGlyTyrValIlePheLeuHisAlaGlyAspLeuAsnThrGly65707580ValProGluSerAspLeuGlnAspAlaIleProAspIleValGlyTyr859095AsnMetIleGlyLeuSerAlaMetAlaValGlyAsnHisGluPheAsp100105110AsnProArgAspValLeuLeuLysGlnMetLysPheAlaAspPhePro115120125PheLeuSerAlaAsnIleLeuLysGluAsnGlyGluProPhePheThr130135140ProTyrIleLeuLysAspPheGlyLysLeuLysValAlaIleIleGly145150155160PheThrThrGluGluThrThrIleLeuGluProLeuTyrLeuGluAsp165170175LeuLysPheGluAsnAlaLeuLysValAlaGlnLysIleAlaProGlu180185190LeuLysAsnGlnAlaAspValValIleAlaLeuAlaHisLeuAspTrp195200205GlyGluProLysLysGluAsnIleThrThrThrHisGlnLeuAlaLys210215220ValAspGlyIleAspValValValAlaGlyHisSerHisValLeuGly225230235240SerGluValValAspGluLysIleIleValSerAlaGlyGluTyrGly245250255LysTyrValGlyArgLeuAspLeuAspIleGluAspGlyLysIleVal260265270AlaTrpHisTrpGlnAlaIleProValAsnLeuLysAlaTyrArgAsp275280285GlyLysTyrGluTyrValGlyLysProTyrLeuGluAsnArgTyrVal290295300AlaLysAlaLeuGluTyrPheArgLysValGlyAsnGluLysLeuAsp305310315320ThrValIleGlyGluThrLysIlePheLeuAspGlyGluArgGluHis325330335ValArgSerArgAsnThrAsnLeuThrAsnLeuIleThrAspAlaMet340345350ArgTrpLysValLysAlaAspIleAlaLeuThrAsnGlyGluGlyIle355360365ArgAlaSerIleLysProGlyLysIleThrValArgAspIleLeuThr370375380ValLeuProPheGlyAsnThrLeuTyrValLeuGluLeuThrGlyGlu385390395400GlnIleMetLysValLeuGluTyrSerAlaThrIleProGluGlyLys405410415GlyAlaPheLeuGlnValSerGlyLeuThrTrpLysSerLysAspGly420425430LysValValGluValLeuValAsnGlyGluProLeuAspProLysLys435440445ThrTyrArgValValThrAsnAsnTyrMetAlaGlyGlyGlyAspGly450455460TyrThrMetLeuLysGluTrpGlyGlyTyrAspThrGlyPheLeuMet465470475480SerAspAlaValIleGluTyrIleGlnAsnValLeuAsnGlyValIle485490495GluGluTyrAspAsnSerGlnArgTyrValArgGlu500505<210>201<211>154<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>201MetAspAlaLysIleValAsnAlaLeuIleGlySerValTyrGluThr151015IleLysSerValLeuArgValGluProLysLeuGlyLysProSerAla202530ValSerHisIleGluIleProHisSerValValThrValIleGlyVal354045ThrGlySerIleGluGlySerLeuIleTyrSerPheSerSerGluThr505560AlaLeuLysValValSerAlaMetMetGlyMetGluTyrGlyGlnLeu65707580AspGluLeuAlaMetSerAlaIleGlyGluLeuGlyAsnMetThrAla859095GlyLysLeuAlaMetLysLeuGluThrLeuGlyLysHisValAspIle100105110ThrProProThrValValSerGlyLysAspLeuLysIleLysSerPhe115120125GlyThrValLeuLysLeuProIleSerValPheSerAsnGluAspPhe130135140AspLeuHisLeuSerValLysSerGlyGly145150<210>202<211>333<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>202MetLysGluIleValAspAlaIleSerGlnTyrAsnArgIleLeuVal151015ValGlyHisIleMetProAspGlyAspCysValSerSerValLeuSer202530LeuThrLeuGlyLeuGluArgIleGlyLysGluValArgAlaAlaIle354045AspTyrArgValProTyrValPheGluAsnPheProHisIleGluArg505560IleGluGlnAsnValAspPheGluProGluLeuValIleValValAsp65707580AlaSerSerProAspArgIleGlyArgPheGlnAspLeuLeuLysGlu859095ValProSerAlaValIleAspHisHisSerThrAsnValLeuPheGly100105110LysTrpAsnTrpValAspProSerPheAlaAlaThrAlaGlnMetVal115120125PheArgLeuAsnArgLysLeuGlyValGluTyrAspProValLeuAla130135140ThrLeuAsnTyrLeuGlyIleAlaThrAspThrGlyPhePheArgHis145150155160SerAsnThrAspAlaArgValPheGluAspAlaTyrGluLeuValLys165170175IleGlyAlaAspAlaHisPheValAlaLysGluIleLeuGluAsnLys180185190ArgPheGluGlnPheLysLeuPheAlaGluValLeuGluArgLeuGln195200205LeuLeuGluAsnGlyArgIleAlaTyrSerTyrIleAspTyrAspThr210215220TyrLeuArgHisAsnCysThrAspGluAspSerAlaGlyPheValGly225230235240GluLeuArgSerIleArgGlyValGluValAlaValLeuPheMetGlu245250255PheProLysGlyLysIleHisValSerMetArgSerLysAspTrpPhe260265270AsnValAsnGluValAlaPheGluLeuGlyGlyGlyGlyHisProArg275280285AlaAlaGlyValThrPheGluGlyGluLysLeuGluAsnValIleSer290295300ArgValIleAspHisLeuLeuGlyArgPheLysGluGlyValSerArg305310315320GluGlyGluLysThrSerGlnArgSerValLeuGlyArg325330<210>203<211>295<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>203MetIleLeuArgArgArgGlyIleValLeuSerPheHisSerAsnMet151015ValThrValGluAspGluGluThrGlyGluArgLeuLeuCysLysLeu202530ArgGlyLysPheArgLeuGlnAsnLeuLysIleTyrValGlyAspArg354045ValGluTyrThrProAspGlyThrGlySerGlyValIleGluAsnVal505560LeuHisArgLysAsnLeuLeuValLysProHisValAlaAsnValAsp65707580GlnAlaIleLeuValValThrValLysMetProGluThrSerThrTyr859095IleIleAspLysPheLeuValLeuThrGluLysAsnGluLeuGluThr100105110ValLeuValIleAsnLysMetAspIleTyrAspGluGluAspLeuGlu115120125LysValLysGluLeuGluArgIleTyrSerLysLeuTyrProIleVal130135140LysThrSerAlaLysThrGlyMetGlyIleGluGluLeuLysLysTyr145150155160LeuLysGlyLysIleSerThrMetAlaGlyLeuSerGlyValGlyLys165170175SerSerLeuLeuAsnAlaIleAsnProGlyLeuLysLeuArgValSer180185190GluValSerGlnLysLeuGlnArgGlyArgHisThrThrThrSerAla195200205GlnLeuLeuArgPheAspPheGlyGlyTyrValValAspThrProGly210215220PheAlaAsnLeuGluIleGlyAspIleGluProGluGluLeuLysTyr225230235240TyrPheLysGluPheAsnGluLysGlnCysPhePheSerAspCysAsn245250255HisIleAspGluProGluCysGlyValLysGluAlaValGluAsnGly260265270GluIleAlaGluSerArgTyrGluAsnTyrValLysMetPheHisGlu275280285LeuLeuGlyArgGlyLysSer290295<210>204<211>528<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>204MetLysLysIleThrGlyArgPheLeuLysThrIleIleLysLysAla151015ThrGluAsnLeuLeuLysHisLysAspGluIleAsnAlaLeuAsnVal202530PheProValProAspGlyAspThrGlySerAsnMetCysSerThrMet354045LeuGluAlaCysLysTyrIleAspAsnLeuLysSerAspAspLeuSer505560GluValTrpLysAlaIleLysGluGlyAlaLeuMetGlyAlaArgGly65707580AsnSerGlyValIleLeuSerGlnIleLeuArgGlyLeuAlaAspAla859095SerProGluAsnTyrIleThrProGlyAspPheIleLysMetIleSer100105110AsnAlaArgLysValAlaTyrSerAlaValMetArgProValGluGly115120125ThrMetLeuThrValValArgGluLeuAspGluLysLeuLysGlyArg130135140SerPheGluThrPheGluGluLeuPheAspGlnIleValGluMetIle145150155160LysAspThrValAsnArgThrProSerMetLeuSerLysLeuArgGlu165170175AlaGlyValValAspAlaGlyAlaLysGlyLeuTyrTyrLeuPheGlu180185190GlyMetArgAspAlaIleLysGlyAspIleGluValAsnLeuGluGln195200205ValGluGlnAlaSerValGluAspLeuLysArgMetAlaLeuGluGlu210215220IleThrAsnGlnTyrCysThrGluValAlaValArgArgLysArgVal225230235240PheGluLysSerGluLeuGluSerPheLeuAsnGluIleGlyAspSer245250255ValValLeuValGluGlnAspAspIlePheArgLeuHisValHisThr260265270AsnHisProGlyGlnValLeuGluLysValLeuAspPheGlyGluIle275280285IleLysValLysValAspAsnMetLysLeuGlnHisGluHisIleIle290295300SerAlaGlnValGluLysGluIleGlyValValAlaValSerProGly305310315320LysGlyIleSerGluIleLeuLysSerLeuGlyValAspGluIleVal325330335ProGlyGlyGlnThrMetAsnProSerPheAlaAspLeuLysAlaAla340345350ValAspLysThrHisAlaLysValValPheLeuPheProAsnAsnAla355360365AsnValLeuLeuThrAlaLysGlnValAlaGluSerValAspAspLys370375380ArgValIleValValGlnThrSerHisValGlnGluCysValAlaAla385390395400MetValGluTyrAspProAspGluAspProGluGluLeuLysArgArg405410415PheGluGluAlaIleAsnGlnCysValProIleSerIleThrArgAla420425430ValArgAspSerArgTyrGlyLysArgArgIleArgLysGlyGluTyr435440445LeuValPheValArgLysGluLeuLeuAlaHisGlyPheAsnLeuVal450455460LysAlaLeuLysAspValLeuGluLysGluAsnAlaArgGluLysGlu465470475480IleLeuThrValPheLeuGlyAspAsnTyrArgLysAlaGluLeuGlu485490495LysIleGlnAsnLeuIleGlyGluGluPheProAsnLeuAspLeuGlu500505510IleHisGluGlyGlyGlnProHisTyrProPheLeuMetLeuLeuGln515520525<210>205<211>263<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>205MetGluGlyGlyIleGluLeuAspArgLeuAspPheSerIleLysLeu151015LeuArgArgValGlyHisPheLeuMetLeuHisTrpGlyLysValAsp202530SerValGluLysLysThrGlyPheLysAspIleValThrGluIleAsp354045LysLysAlaGlnGluMetIleValGluGluIleArgLysValPhePro505560AspGluAsnIleIleAlaGluGluGlyIleSerGluAsnGlyLysLys65707580LeuTrpIleIleAspProIleAspGlyThrIleAsnPheValHisGly859095LeuProAsnPheSerIleSerIleAlaTyrValGluAsnGlyGluVal100105110LysMetGlyValValHisAlaProAlaLeuAsnGluThrLeuTyrAla115120125GluGluAsnGlyGlyAlaPheLeuAsnGlyGluArgIleArgValSer130135140GlyAsnThrSerLeuGluGluCysValGlySerThrGlySerTyrVal145150155160AspPheThrGlyLysPheIleGluLysMetGluLysLysThrArgArg165170175ValArgIleLeuGlySerAlaAlaLeuAsnAlaCysTyrValGlyAla180185190GlyArgValAspPhePheValThrTrpArgIleAsnProTrpAspIle195200205AlaAlaGlyLeuIleValValLysGluAlaGlyGlyThrValThrAsp210215220PheAlaGlyLysGluAlaAsnValPheSerLysAsnPheValPheSer225230235240AsnGlyLeuValHisGluGluValLeuGluValValAsnGluValLeu245250255LysGluIleGlyGluGlyLys260<210>206<211>90<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>206MetLysAlaLeuLysIleArgValGluGlyIleValGlnGlyValGly151015PheArgTyrPheThrArgArgIleAlaArgThrLeuGlyIleLysGly202530TyrValMetAsnMetAspAspGlySerValPheIleHisAlaGluGly354045GluGluGluLysLeuArgArgPheLeuAsnGluValSerLysGlyPro505560ProAlaAlaValValThrAsnIleSerValGluGluThrSerProGlu65707580GlyTyrGluAspPheAlaIleArgTyrTyr8590<210>207<211>401<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>207MetLeuSerPhePheCysGluThrProPheGlnIleLeuProTyrGly151015LysGluGluLeuAspSerMetGlnLeuGluArgLeuAspGluGlyLeu202530PheTyrProHisTyrAsnGlyTyrSerIleValAsnPheSerAsnThr354045IleLeuSerIlePheGlyGluLysThrLeuHisArgProPheProLeu505560ProGluArgPheLeuGluAsnValArgLysValValValLeuValVal65707580AspAlaLeuSerTyrAspValPheSerAsnThrIleGlnAspAspAsp859095ValGlnValPheProCysSerSerValPheProThrThrThrAlaAla100105110AlaLeuProSerLeuTyrSerGlyLeuThrProLeuGluHisGlyPhe115120125LeuGlyTyrIleLeuTyrLeuArgGluValGlySerProValAsnMet130135140IleGluMetAlaProProGlyPheProArgGluSerValLeuArgIle145150155160HisGluPheArgPheThrThrIlePheGlnArgLeuLysValArgSer165170175PhePheLeuValProArgTyrLeuLeuGlyThrGlyPheSerArgLeu180185190MetSerGlnGlyAlaGluGlnIleGlyPheSerSerPheGlyAspMet195200205IleGluLysThrLeuGluIleLeuAlaAsnGlnGluThrValLeuVal210215220PheAlaTyrTrpProSerLeuAspSerIleAlaHisLysSerGlyVal225230235240GlyArgAlaTyrPheArgGluLeuLysTrpLeuTyrArgIleLeuLys245250255GluGluLeuIleArgArgLeuLysSerAspThrLeuPhePheMetVal260265270SerAspHisGlyGlnIleSerThrProProGluArgGluValTrpTrp275280285AspSerPheSerGluValMetArgPheLeuAspArgProProAlaGly290295300GluGlnArgMetMetTyrLeuTyrThrLysArgLysLysAlaLeuVal305310315320GluTyrLeuValGluLysTyrAlaAspPheAlaIlePheIleAspAla325330335ArgArgAlaThrArgLeuPheGlyIleGlyLysSerHisProGluPhe340345350PheHisArgIleGlyAspLeuIleLeuIleThrLysGluAsnPheSer355360365PheAsnTyrArgTyrThrGlyLysGluGluSerLeuSerGlyArgHis370375380GlySerLeuThrHisGlnGluLeuValValProLeuValValTyrArg385390395400Arg<210>208<211>229<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>208MetLysAlaAspLeuHisValHisThrCysLeuSerProCysAlaAsp151015LeuLeuMetIleProProValIleGluLysAlaGluValAspIleLeu202530GlyIleValAspHisAsnSerAlaArgAsnAlaLeuAlaPheSerLys354045MetLysLysLeuValIleProGlyValGluIleGlnThrValGluAsp505560ValHisLeuLeuGlyPhePheValAsnPheGluAspAlaLeuLysLeu65707580ThrGluValLeuTyrGluHisLeuProArgLeuLysHisAspHisGlu859095LysMetGlyTyrGlnLeuLeuValAsnGluAspGlyAspTyrIleGly100105110TyrGluAsnMetProLeuGlyPheProAlaAsnLeuThrIleSerGln115120125AlaValAspLeuValLeuSerPheArgGlyValProAlaTyrAlaHis130135140ValGluArgArgPheGlyValLeuTyrGlnLeuGlyPhePheProAsn145150155160LeuAspValProValAlaGluValValSerGluGluGlyArgSerLys165170175AlaGluGlyArgPheArgValIleValSerSerAspAlaHisPhePro180185190AspGluValGlyArgArgTyrIleGluLeuLysGlyLysProAspSer195200205ProLysLysValLeuAspGlnIleLeuAlaGlyGluTyrThrLeuGly210215220GlyValLeuAspTrp225<210>209<211>122<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>209MetLeuAspValTrpLysIlePheArgSerThrProPheThrThrAla151015ValValSerPheLeuValAlaGlnPheIleLysPheLeuIleLysArg202530AspValLysMetLeuLysSerTyrGlyGlyMetProSerGlyHisVal354045AlaThrValSerGlyLeuAlaTrpSerLeuAlaArgSerThrGlyPhe505560AspSerProTyrThrSerIleAlaSerIlePheLeuValIleIlePhe65707580MetAspAlaIleValLeuArgProAlaValLysLysAspLeuGlyHis859095SerPheLeuGluAlaLeuAlaGlyPheGlyLeuGlyMetLeuIleAla100105110HisLeuPheProAlaArgLeuHisLeuTrp115120<210>210<211>181<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>210MetThrValLysPheTyrGluGlnLysIleGluSerLysArgValPhe151015GluGlyLysMetIleSerValArgValAspArgValArgLeuProAsn202530GlyGluGluSerThrArgGluValValAspHisProGlyAlaValVal354045IleValProValLeuGlyGluGluIleIlePheValGluGlnTyrArg505560TyrProIleGluGlnMetLeuLeuGluLeuProAlaGlyLysMetAsp65707580ProGlyGluSerProGluGluCysAlaLysArgGluLeuGluGluGlu859095ThrGlyTyrArgAlaLysArgPheSerTyrLeuGlyLysIlePheThr100105110ThrProGlyPheThrThrGluValIleHisIlePheAlaAlaGluGlu115120125LeuGluLysThrThrGlnAsnThrAspProAspGluPheIleGluVal130135140LysLysIleProValGluLysValLeuSerLeuLeuArgAsnAlaGlu145150155160IleGluAspSerLysThrIleCysAlaLeuThrArgTyrPheLeuSer165170175LysGlyValIleGly180<210>211<211>224<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>211MetLysLysAlaValLeuPheAspLeuAspGlyThrIleLeuAspPhe151015GluLysSerGluGluThrAlaLeuLysLysThrPheLeuArgHisGly202530IleProLeuThrGluGluGlnValLeuLeuTyrLysSerIleAsnArg354045LysTrpTrpLysMetLeuAlaGluLysLysValSerLysGluGluVal505560ValValAlaArgPheGluGluPheLeuGlyGluIleGlySerLeuLeu65707580AspProGluGluValAlaLysGluTyrLeuGluPheLeuSerGluGlu859095AlaTyrPheLeuProGlyAlaGluAspPheLeuLysGluLeuLysArg100105110AsnGlyPheArgMetAlaAlaValThrAsnGlyValArgPheValGln115120125GluArgArgSerLysLysLeuGlyLeuGluArgPhePheGluPheVal130135140LeuThrSerGluGluValGlyValGluLysProAspProArgIlePhe145150155160TrpIleAlaLeuGluArgMetGlyLeuLysLysGluAspAlaLeuTyr165170175ValGlyAspAspProAlaSerAspLeuGluGlyAlaArgAsnAlaGly180185190IleAspPheValLeuPheSerProSerGlyAspValSerArgGluPhe195200205ProValValArgAsnPheGluGluLeuArgGluIleLeuArgIleLeu210215220<210>212<211>204<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>212MetAsnIleSerGluArgGlnLysAspLeuLeuLysGluIleGlyAsn151015IleGlyAlaGlyAsnAlaAlaThrAlaIleSerTyrMetValAsnLys202530LysValGluIleSerValProThrValGluIleIleProLeuSerAsp354045ValIlePheValAlaLysAspProGluGluIleValValGlyValLys505560MetProValThrGlyGluIleGluGlyAsnValLeuLeuIleMetGly65707580ThrGlnValValLysLysIleLeuGluIleLeuIleGlyHisAlaPro859095GluAsnLeuLeuGlnLeuAspGluPheAlaAlaSerAlaLeuGlnGlu100105110ValGlyAsnIleMetCysGlyThrTyrValSerAlaLeuAlaAspPhe115120125LeuGlyPheLysIleAspThrLeuProProGlnLeuValValAspMet130135140IleSerAlaIlePheAlaGluValSerIleGluGluLeuGlyGluSer145150155160ValAspAspGlnValValPheValGluThrSerLeuThrValGluGlu165170175GluGluProLeuThrSerTyrLeuMetLeuValProLysProGlyTyr180185190LeuLysLysIlePheGluArgMetGlyValGlnGlu195200<210>213<211>236<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>213MetGluMetLeuLeuGlyIleValGlnGlyLeuThrGluPheLeuPro151015ValSerSerSerGlyHisLeuValLeuPheSerHisValPheLysMet202530AspLeuAsnAlaTyrGlnThrAlaIleLeuHisLeuGlyThrLeuAla354045AlaValValValPheAlaLeuGluGlyIleArgArgSerLeuArgAsn505560TrpArgValIleLeuAsnLeuValIleSerThrIleProAlaGlyVal65707580PheGlyValLeuPheGluSerArgValAspGluAlaPheSerSerPro859095ValValLeuProLeuPhePheSerPheThrAlaLeuValLeuLeuPhe100105110ThrSerSerPheSerGlyGluLysArgMetGluGluMetThrIleLeu115120125AspAlaThrLeuValGlyLeuAlaGlnValLeuAlaLeuPheProGly130135140ValSerArgSerGlyIleThrIleAlaThrLeuLeuPheLeuLysTyr145150155160ArgArgGluAspAlaLeuGlnTyrSerPheLeuMetSerIleProVal165170175ValLeuGlyAlaGlyIleLeuGlyMetGluLysGlyAsnIleSerLeu180185190ThrAlaProPhePheAlaPheLeuSerGlyIleLeuAlaLeuTyrIle195200205LeuSerArgSerValArgSerGlyLysMetTrpGlnPheAlaTyrTyr210215220CysLeuPheValAlaIleValSerTyrPheValGly225230235<210>214<211>233<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>214MetIleAspMetHisLeuHisSerThrPheSerTyrAspGlyLysAla151015GluValGluAspIleIleAlaGlnValGlnLysLeuGlyIleAsnHis202530PheCysIleThrAspHisTyrGluTyrGluAsnGlyLysLeuValHis354045AspPheAsnValGluGluTyrPheLeuThrMetAspLysPheAspLeu505560ProArgGlyAlaGluIleSerTrpAspGlyValLysGluAlaIlePhe65707580ProGluGlyPheAspTyrLeuLeuLeuGlyValHisArgTyrAspGlu859095AsnLeuProProAspGluLeuAlaArgAspTyrLeuGluArgThrLeu100105110TyrValMetGluArgValGluPheHisThrLeuAlaHisLeuAspTyr115120125ProAlaArgTyrAlaAsnAlaAspPheLysAlaAsnArgAspLeuIle130135140GluLysIleLeuArgPheLeuValAlaArgGluLysIleLeuGluVal145150155160AsnThrAlaGlyLeuPheLysHisGlyLysProAsnProAspTyrTrp165170175IleLeuGluMetTyrTrpAspLeuGlyGlyArgPheIleThrIleGly180185190SerAspAlaHisGluValGlnHisIleGlyArgGlyIleGluGluVal195200205MetSerLysLeuArgArgPheAsnPheGluTyrValValValGluGly210215220LysLysLeuPheThrLysLysLeuArg225230<210>215<211>227<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>215MetValAsnValValMetValProCysSerValValLysAspGluThr151015ValValValValAspValLeuArgAlaThrSerThrIleValThrAla202530LeuAlaAsnGlyAlaLysGluValIleProValLysThrValGluGlu354045AlaLeuLysArgLysGluArgAspValLeuValCysGlyGluArgAsn505560AlaLysLysIleGluGlyPheAspLeuGlyAsnSerProLeuGluTyr65707580LysSerGluLeuValLeuGlyLysThrIleValLeuThrThrThrAsn859095GlyThrGlnValIleGluArgValGluGlyAspSerValIleAlaAla100105110SerPheLeuAsnValSerAlaValValGluTyrLeuLysGluLysGlu115120125AsnIleThrIleValCysAlaGlyThrSerGlyAspPheSerLeuGlu130135140AspPheLeuLeuAlaGlyMetIleValLysArgMetLysArgGluAsp145150155160LeuLeuAspGlyAlaLeuValValMetArgTyrPheGluSerValGln165170175AsnIleArgGluGluIleLysArgPheSerSerHisAlaLysArgLeu180185190IleSerLeuGlyPheGluLysAspValAspPheCysThrThrGluAsp195200205LeuPheHisGluValProValLeuValAsnGlyAlaPheThrLeuLys210215220GluAlaArg225<210>216<211>209<212>PRT<213>那不勒斯栖热袍菌(Thermotoganeapolitana)<400>216MetThrTyrAlaValGlyAspIleHisGlyCysPhePheAlaLeuLys151015AlaLeuLeuGluLysLeuProLeuGlyLysGluAspGluLeuValPhe202530LeuGlyAspTyrValAspArgGlyProAsnSerLysGluValValGlu354045PheLeuMetGluLeuSerLysHisHisArgCysIlePheLeuArgGly505560AsnHisGluGluMetLeuLeuAsnCysValLysAsnHisSerGlyCys65707580AspLeuTrpTyrPheAsnGlyAlaArgSerThrValGluSerPheGly859095GlyIleAspGluIleArgLysTyrLeuAspPhePheGluSerThrVal100105110TyrTyrTyrGluLysGlyAsnPheValPheValHisGlyGlyValLys115120125ProGlyIleProLeuGluGluGlnAspProPheAspLeuValTrpIle130135140ArgGluGluPheIleTyrSerGluAsnProLeuProGlyLysThrVal145150155160ValPheGlyHisThrProLeuGluLysProTyrValSerSerAspLys165170175IleGlyIleAspThrGlyCysValTyrGlyGlyLysLeuThrAlaPhe180185190ArgValGluAspArgThrPhePheGlnValGluCysIleGlyArgArg195200205Trp<210>217<211>264<212>PRT<213>嗜石油热袍菌(Thermotogapetrophilia)<400>217MetPheValPheAspLeuAspGlyThrLeuLeuAsnAspSerLeuGlu151015IleSerGluLysAspArgArgAlaIleGluArgLeuSerArgLysCys202530ArgValValPheAlaSerGlyArgMetLeuValSerThrLeuAsnVal354045GluLysLysTyrPheGlyArgThrPheProThrIleAlaTyrAsnGly505560AlaMetValTyrIleProGluGluGlyValIleLeuAsnGluLysIle65707580ProProGluValAlaLysAspIleValGluTyrValLysGlnPheAsn859095ValHisTrpGlnAlaTyrIleAspAspValLeuTyrSerGluLysAsp100105110AsnGluGluIleArgGlyTyrAlaLysHisSerSerValGluTyrArg115120125ValGluProLysLeuLeuGluLeuValSerLysValGlyThrThrLys130135140IleLeuLeuIleAspThrProGluLysLeuAspGluLeuLysLysIle145150155160LeuLeuGluLysPheAsnAsnValValLysValPheLysSerPhePro165170175ThrTyrLeuGluIleValProGluAsnValAspLysGlyLysAlaLeu180185190ArgPheLeuArgGluArgMetGlyTrpLysLysGluGluIleValVal195200205PheGlyAspAsnGluAsnAspLeuPheMetPheGluGluAlaGlyLeu210215220ArgValAlaMetGlyAsnAlaIleAspLysValLysGluAlaAlaAsp225230235240ValValThrLeuThrAsnAsnAspSerGlyValSerHisValLeuGlu245250255LeuIleSerThrAspCysLeuAsp260<210>218<211>208<212>PRT<213>Thermusamyloliquefaciens<400>218MetLeuLysAlaLeuLeuPheAspLeuAspGlyThrLeuAlaAspThr151015AspProLeuHisLeuGlnAlaTrpArgGluAlaLeuAlaProTrpGly202530LeuGlnValAspGluAlaPheTyrArgLysArgIleSerGlyArgLeu354045AsnProAspIleValGlnAspLeuLeuGlyLeuGluGlyGluAlaAla505560GlnArgLeuIleGluAlaLysGluAlaArgPheArgGluLeuAlaGln65707580AspLeuLysProThrProGlyLeuHisGlyLeuLeuAlaLysAlaGlu859095AlaGlnGlyLeuThrTrpGlyValValThrAsnAlaProArgGluAsn100105110AlaLeuHisValLeuArgAlaLeuGlyLeuAsnProProLeuLeuVal115120125LeuAlaGluGluValGlyArgGlyLysProAspProLeuProTyrArg130135140ValAlaLeuGluArgLeuGlyIleGluProGlyGluAlaLeuAlaPhe145150155160GluAspSerProSerGlyValArgSerAlaValGlyAlaGlyIlePro165170175ThrTyrAlaLeuLeuThrGlyHisGlyLysGluAlaLeuLeuGluAla180185190GlyAlaLeuGluAlaLeuArgAspPheGlnGlnIleValAspLeuLeu195200205<210>219<211>260<212>PRT<213>丝状栖热菌(Thermusfiliformis)<400>219MetLysValSerGlnAlaHisLeuGluAlaAlaIleAspAlaAlaPhe151015LeuAlaGlnGlyIleHisThrTyrTyrLeuGluArgGlyPheThrGln202530LysThrLysSerGlyProThrAspLeuValThrGlnAlaAspLysGlu354045SerGluGluAlaIleLysAspLeuLeuLeuSerArgPheProAspHis505560GlyPheLeuGlyGluGluGlyGlyGlyAspAlaGlyGlyGlyValArg65707580TrpValValAspProLeuAspGlyThrValAsnTyrAlaHisGlyPhe859095ProPheTyrGlyValSerIleAlaLeuGluValGluGlyArgValGlu100105110LeuGlyValValLeuAspThrAlaArgGlyGluLeuPheThrAlaLeu115120125ArgGlyGlnGlyAlaTyrLeuAsnGlyArgProIleArgValThrGlu130135140ArgThrGluLeuLeuGlySerLeuLeuAlaThrGlyPheProTyrAsp145150155160ValAlaArgAspProGluAsnLeuThrTyrPheGluArgAlaLeuArg165170175ArgGlyLeuLeuValArgArgProGlyAlaAlaAlaLeuAspLeuAla180185190TyrValAlaAlaGlyArgLeuAspGlyPheTrpGluValLysLeuAsn195200205ProTrpAspValAlaAlaGlyTrpLeuLeuValGluGluAlaGlyGly210215220ArgValThrAspLeuGluGlyGluAlaTyrArgLeuGlyHisArgTyr225230235240IleValAlaThrAsnGlyArgIleHisGlnAlaLeuLeuAlaValLeu245250255ArgGlyThrMet260<210>220<211>208<212>PRT<213>嗜热栖热菌(Thermusthermophilus)<400>220MetLeuLysAlaValLeuPheAspLeuAspGlyThrLeuAlaAspThr151015AspProLeuHisLeuLeuAlaTrpArgGluAlaLeuAlaProTyrGly202530LeuGluValAspProIlePheTyrArgLysArgIleSerGlyArgLeu354045AsnProGluIleValArgAspLeuLeuGlyLeuGluGlyGluGluAla505560GluArgLeuIleAlaAlaLysGluAlaArgPheArgAlaLeuAlaGln65707580GlyLeuArgProThrProGlyLeuAlaGluPheLeuGluHisIleArg859095GluLysGlyLeuLeuTrpGlyValValThrAsnAlaProLysGluAsn100105110AlaArgHisValLeuGluAlaLeuGlyLeuArgProProLeuLeuVal115120125LeuAlaGluGluValGlyArgGlyLysProAspProLeuProTyrArg130135140ValAlaLeuArgArgLeuGlyValAlaProGluGluAlaLeuAlaPhe145150155160GluAspSerProSerGlyValArgSerAlaValGlyAlaGlyIlePro165170175ThrTyrGlyLeuLeuThrGlyHisGluAlaGluAlaLeuArgGluAla180185190GlyAlaArgGlyValPheArgAspPheArgGluAlaLeuGlyLeuLeu195200205<210>221<211>264<212>PRT<213>嗜热栖热菌(Thermusthermophilus)<400>221MetIleGlyArgArgHisProTyrTyrProTyrLeuGluAlaAlaLeu151015GluAlaAlaSerLeuAlaArgGlyIleHisLeuTyrTyrLeuGluLys202530GlyPheThrGluGlyThrLysSerGlyProThrAspLeuValThrGln354045AlaAspArgGluAlaGluGluAlaValLysGlyLeuLeuLeuSerArg505560PheProGluAlaGlyPheLeuGlyGluGluGlyGlySerGluGlyGly65707580LysAlaLeuArgPheIleValAspProLeuAspGlyThrValAsnTyr859095AlaHisGlyPheProPhePheAlaValSerIleAlaLeuGluAlaGlu100105110GlyAlaIleGlnMetGlyValValMetAspThrAlaArgGlyGluVal115120125PheTyrAlaLeuArgGlyGluGlyAlaTyrLeuAsnGlyArgProIle130135140ArgValThrGlyArgGluSerLeuValGlySerLeuLeuAlaThrGly145150155160PheProTyrAspValAlaLysAspProGluAsnLeuThrTyrPheGlu165170175ArgAlaLeuGlyLysGlyLeuLeuValArgArgProGlyAlaAlaAla180185190LeuAspLeuAlaTyrValAlaAlaGlyArgLeuGluGlyPheTrpGlu195200205ValLysLeuAsnProTrpAspValAlaAlaGlyTrpLeuLeuValGlu210215220GluAlaGlyGlyArgValThrAspLeuGluGlyAsnProTyrArgLeu225230235240GlySerArgTyrIleLeuAlaThrAsnGlyArgValHisGluAlaLeu245250255ArgArgThrLeuLeuGlyLeuAsp260<210>222<211>229<212>PRT<213>Trueperaradiovictrix<400>222MetGlyHisProLysAlaValLeuLeuAspValAspGlyThrLeuVal151015AspSerAsnAspAlaHisAlaArgAlaTyrValAspAlaLeuLysAla202530TyrGlyTyrAspValProPheGluLysValArgProLeuIleGlyMet354045GlyGlyAspLysLeuLeuProGlnValIleAspValGluSerAspAla505560ProLeuArgGluGluIleAspAlaArgArgGlyGluIlePheLysSer65707580ArgTyrLeuProAspIleLysProPheProGlyValArgGluLeuLeu859095GluArgLeuArgArgAspGlyLeuThrLeuValAlaAlaSerSerAla100105110LysGluAspGluAlaAspAlaLeuLeuGluArgAlaGlyValAlaAsp115120125LeuIleAspAlaArgThrSerSerAspAspAlaGluAsnSerLysPro130135140AspProAspIleLeuGlnAlaAlaLeuGluArgAlaGlyValSerAla145150155160GlnGluAlaLeuLeuLeuGlyAspThrProTyrAspLeuGlnAlaAla165170175AlaProLeuGlyLeuGlyValValAlaLeuArgCysGlyGlyTrpArg180185190AspGluAspLeuLeuGlyAlaLeuAlaValTyrAspAspProGlnAla195200205LeuLeuAlaGlyTyrAspAlaSerProLeuAlaProGluProAlaSer210215220AlaProGlyAspVal225当前第1页12